Several approaches are presently being developed for global proteome characterization that are based upon the analysis of polypeptide mixtures resulting from digestion of (often complex) mixtures of proteins. Improved methods for peptide analysis are needed that provide for sample concentration, higher resolution separations, and direct compatibility with mass spectrometry. In this work methods for the high-efficiency capillary isoelectric focusing (CIEF) separation of peptides have been developed. CIEF of peptides provide simultaneous sample concentration and separation according to peptide isoelectric points. Under typical non-denaturing CIEF conditions, peptides are concentrated -500-fold, and peptides present at
Revised: March 16, 2001 |
Published: May 15, 2000
Citation
Shen Y., S.J. Berger, G.A. Anderson, and R.D. Smith. 2000.High-Efficiency Capillary Isoelectric Focusing of Peptides.Analytical Chemistry 72, no. 9:2154-2159.PNNL-SA-32454.