June 9, 2005
Journal Article

High-Dose Estrogen and Clinical Selective Estrogen Receptor Modulators Induce Growth Arrest, p21, and p53 in Primate Ovarian Surface Epithelial Cells

Abstract

Ovarian cancer is the most lethal gynecological cancer affecting women. Hormone-based therapies are variably successful in treating ovarian cancer, but the reasoning behind these therapies is paradoxical. Clinical reagents such as tamoxifen are considered to inhibit or reverse tumor growth by competitive inhibition of the estrogen receptor (ER); howerver high dose estrogin is as clinicaly effective as tamoxifen, and it is unlikely that estrogen is acting by blocking ER activity; however, it may be activating a unique funciton of the ER that is nonmitogenic. For poorly defined reasons, 90% of varian cancers derive from the ovarian surface epithelium (OSE. In vivo the ER-positive OSE is exposed to hight estrogen levels, reaching micromoloar concentrations in dominant ovarian follicles. Using cuItured OSE cells in vitro, we sho that these levels of estradiol (1 ug/ml; ~3um) block the actions of serum growth factors, activate the G1 phase retinoblastoma AQ:A checkpoint, and induce p21, an inhibitor of kinases that normally inactivate the retinoblastoma checkpoint. We also show that estradiol increases p53 levels, which may contribute to p21 induction. Supporting the hypothesis that clinical selective ER modulators activate this novel ER function, we find that micromolar doses of tamoxifen and the "pure antiestrogen" ICI 182,780 elicit the same effects as estradiol. We propose that, in the context of proliferation, these data clarify some paradoxical aspects of hormone-based therapy and suggest that fuller understanding of normal ER function is necessary to improve therapeutic strategies that target the ER. (J Clin Endocrinol Metab 90: 0000-0000, 2005)

Revised: October 25, 2005 | Published: June 9, 2005

Citation

Wright J.W., R.L. Stouffer, and K.D. Rodland. 2005. High-Dose Estrogen and Clinical Selective Estrogen Receptor Modulators Induce Growth Arrest, p21, and p53 in Primate Ovarian Surface Epithelial Cells. Journal of Clinical Endocrinology and Metabolism 90, no. 6:3688-3695. PNNL-SA-45549.