Fusing Quantitative-Phase Imaging with Airy Light- Sheet Microscopy
Abstract
We report the integration of quantitative-phase imaging (QPI) with light-sheet (LS) fluorescent microscopy on to a standard inverted microscope that retains compatibility with microfluidics. QPI enables label-free imaging and number-density quantification of single cells and their organelles. Conversely, LS yields considerable speed and phototoxicity gains in quantifying the 4D dynamics of gene-encoded fluorescent biomarkers. We will detail the system design that relied on spatial light interferometry for QPI and an accelerating Airy- beam light-sheet for fluorescence, its performance, as well as results of a representative multivariate imaging analysis of single-cell metabolism.
Published: May 1, 2021
Citation
Subedi N.R., P.S. Jung, E.L. Bredeweg, S.H. Nemati, S.E. Baker, D.N. Christodoulides, and A.E. Vasdekis. 2021."Fusing Quantitative-Phase Imaging with Airy Light- Sheet Microscopy." In Quantitative Phase Imaging VII, March 6-12, 2021. Online. Proceedings of the SPIE, edited by Y. Liu, G. Popescu and Y.K. Park, 11653, Paper No. 116530I. Bellingham, Washington:SPIE.PNNL-SA-160791.doi:10.1117/12.2583926