PNNL

Abstract

Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) is playing an increasing role in the characterization of cellular systems due to its capabilities for providing higher confidence of identification, increased dynamic range, and sensitivity unmatched by other MS platforms. Particularly in Proteomics, where global and quantitative approaches are essential, the attributes of FTICR-MS are poised to make significant contributions. This review covers the recent advances in the field with an emphasis on proteomic applications. It includes high performance microcolumn separation techniques coupled to FTICR, as well as methods that improve protein identification, sensitivity, dynamic range, and throughput.