March 17, 2004
Journal Article

Dissociation Behavior of Doubly-Charged Tryptic Peptides: Correlation of Gas-Phase Cleavage Abundance with Ramachandran Plots

Abstract

Large numbers of gas-phase dissociation spectra of protonated peptides are obtained daily and used in protein identification studies. Yet fundamental knowledge of the factors that influence their unimolecular dissociation branching ratios is relatively poor. It is still not possible to predict dissociation branching ratios from peptide sequence. Clearly, several chemicals factors must influence dissociation patterns, includes y, f angles determined by the residues involved in an amide bond, the propensities for certain side chains to interact each other or with the backbone, the tendency for added protons to be intramolecularly solvated, and the stability of the fragment ions once formed.

Revised: October 25, 2005 | Published: March 17, 2004

Citation

Huang Y., J.M. Triscari, L. Pasa-Tolic, G.A. Anderson, M.S. Lipton, R.D. Smith, and V.H. Wysocki. 2004. Dissociation Behavior of Doubly-Charged Tryptic Peptides: Correlation of Gas-Phase Cleavage Abundance with Ramachandran Plots. Journal of the American Chemical Society 126, no. 10:3034-3035. PNNL-SA-43138.