As an innovative way of enzyme stabilization, we recently developed a new enzyme composite of nano-meter scale that we call "single-enzyme nanoparticles (SENs)" (9). Each enzyme molecule is surrounded with a porous composite organic/inorganic network of less than a few nanometers think. This approach represents a new type of enzyme-containing nanostructure. In experiments with perotease (chymotrypsin, CT), the activity of single enzyme nanoparticle form of the enzyme was greatly stabilized compared to the free form, without imposing a serious mass transfer limitation of substrates. In this chapter we will describe the synthesis, characterization and catalytic activity of the new SENs.
Revised: July 13, 2011 |
Published: March 31, 2004
Citation
Grate J.W., and J. Kim. 2004.Biocatalytic Single Enzyme Nanoparticles. In Dekker Encyclopedia of Nanoscience and Nanotechnology, edited by James A. Schwarz, Cristian I. Contescu, and Karol Putyera. 235-239. New York, New York:Marcel Dekker.PNNL-SA-39690.