December 1, 2003
Journal Article

Assessment of Bioavailability Limitations During Slurry Biodegradation of Petroleum Hydrocarbons in Aged Soils

Abstract

In an effort to determine whether bioavailability limitations are responsible for the slow or incomplete hydrocarbon biodegradation in aged soils, both the rate of desorption (rdes) and biodegradation (rbio) was measured for n-alkanes and polynuclear aromatic hydrocarbons (PAHs) at different times during the slurry biotreatment of six different soils. While all n-alkanes were biodegraded to various degrees depending on their respective carbon number and the soil organic matter content, none of them were desorbed to a significant extent indicating that these saturated hydrocarbons do not need to be transferred from the soil particles into the aqueous phase in order to be metabolized by microorganisms. Most 2 and 3 ring PAHs biodegraded as fast as they were desorbed (rbio=rdes), i.e., desorption rates controlled biodegradation rates. By contrast, the biodegradation kinetics of 4, 5, and 6 ring PAHs was limited by microbial factors during the initial phase (rbio>0) but was more likely caused by microbial factors such as the absence of specific PAH degraders or cometabolic substrates. Consequently, PAHs that are found to be microbially recalcitrant in aged soils may not be so because of limited bioavailability and thus could pose a greater risk to the environment than previously thought.

Revised: April 6, 2004 | Published: December 1, 2003

Citation

Huesemann M.H., T.S. Hausmann, and T.J. Fortman. 2003. Assessment of Bioavailability Limitations During Slurry Biodegradation of Petroleum Hydrocarbons in Aged Soils. Environmental Toxicology and Chemistry 22, no. 12:2853-2860. PNNL-SA-40844.