We identified eight bands by staining the native gels for NADPH-nitroblue tetrazolium oxidoreductase activity after electrophoresis of n-dodecyl-ß-maltoside-treated membranes of Synechocystis sp. strain PCC 6803. Among them, bands A, C, D and E were attributed to the activity of NADPH dehydrogenase (NDH-1). Band A is a highly active super-complex of NDH-1 (about 1,000 kDa) that was absent in ?ndhD1/D2 mutant and was suppressed under low CO2. Band C was induced under low CO2 or in ?ndhD1/D2 mutant and was converted to bands D and E. Bands A and C appear to be NDH-1L dimer and NDH-1M, respectively, with subunits essential for the activity.
Revised: April 7, 2011 |
Published: October 1, 2006
Citation
Ma W., Y. Deng, T. Ogawa, and H. Mi. 2006.Active NDH-1 complexes from the cyanobacterium Synechocystis sp. strain PCC 6803.Plant & Cell Physiology 47, no. 10:1432-1346.PNNL-SA-53863.doi:10.1093/pcp/pcl008