Online control of living cell signal propagation experiments is being facilitated with field programmable gate array (FPGA) processing. The technology approach captures confocal fluorescence microscopy imagery in a manner that real-time, live-cell chemical signaling can be monitored during an experiment for extended time periods. The experiments obtain quantitative, spatial characteristics of the cell chemistry from the imagery through analysis, following a localized perturbation stimulus. The construction of a high-speed, confocal microscope provides simultaneous, two color image acquisition, at speeds of up to 30 fps, allowing near-real-time fluorescent resonance energy transfer and subsequent ratiometric, chemical sigaling analysis. FPGA provides real-time image processing hardware for obsering adjustments of the microscope settings as the images are received, using real-time image registration and fusion algorithms displayed through a user interface.
Revised: October 7, 2005 |
Published: May 24, 2004
Citation
Resat M.S., J.C. Solinsky, H.S. Wiley, K.A. Perrine, T.A. Seim, and S.E. Budge. 2004.3-D Multispectral Monitoring of Living-Cell Signaling Using Confocal-Imaging and FPGA Processing. In International Symposium on Biomedical Imaging, 2004 Proceedings, IEEE, 1, 680-683. Piscataway, New Jersey:IEEE.PNNL-SA-41577.