Pacific Northwest Cryo-EM Center

Training the next generation of cryo-electron microscopy specialists and providing free access to state-of-the-art structure determination

compilation of structures of macromolecular complexes

Researchers from Pacific Northwest National Laboratory and Oregon Health & Science University are providing specialized training in cryo-electron microscopy through the Pacific Northwest Cryo-EM Center. 

(Composite image by James Evans | Pacific Northwest National Laboratory)

The Pacific Northwest Center for Cryo-EM (PNCC) is a national user facility for cryo-electron microscopy that is jointly operated by Oregon Health & Sciences University (OHSU) and Pacific Northwest National Laboratory (PNNL). Funding is provided by the National Institutes of Health Common Fund (NIH).

PNCC provides new and experienced cryo-EM researchers with free access to state-of-the-art workflows for single particle analysis and electron tomography. The center also offers individual and group training to users related to:

  • sample analysis and optimization
  • grid preparation and screening
  • (semi-)automated data collection
  • image analysis and 3D reconstruction

Since 2019, PNCC has served over 800 unique users from 110 institutions, has collected over 12,000,000 cryo-EM movies, and has helped determine the structure of over 400 macromolecular complexes reported in peer-reviewed publications.

PNCC is one of the collaborative projects under the umbrella of the Pacific northwest bioMedical Innovation Co-laboratory (PMedlC).

PMedlC's mission is to use multidimensional omics and imaging data to improve understanding around disease and to inform customized treatment options for patients.