Senior Research Scientist, Global Security Technology & Policy
Senior Research Scientist, Global Security Technology & Policy


Dr. Rachel Bartholomew is a Senior Research Scientist and Technical Team Lead in the Global Security, Technology, and Policy Group at Pacific Northwest National Laboratory. She has 20 years of experience applying molecular biology to address challenges related to national security, policy and non-proliferation. She has led projects for a variety of sponsors, including the Departments of Homeland Security, Energy, State, and Defense, on topics ranging from the development and testing biological agent characterization methods and testing to serving as a biological subject matter expert supporting training on the awareness, interdiction, and mitigation of low-technology threats on public transportation for international partners. Rachel is responsible for compiling the annual Biological Weapons Convention Confidence Building Measures for PNNL. She has recent publications in synthetic biology supply chain, a novel tool for U.S biodefense policy analysis and visualization (Biodefense Policy Landscape Analysis Tool), and the evaluation of current off-the-shelf agent detection kits. She received her Bachelor of Arts in biology from Case Western Reserve University and PhD in animal physiology from Cornell University, where she was a National Science Foundation Pre-Doctoral Fellow. Her post-doctoral training was completed at the FBI Laboratory Counterterrorism and Forensic Science Research Unit. 

Disciplines and Skills

Biosecurity, Biodefense, Molecular Biology, Microbiology, Testing and Evaluation


PhD, Animal Physiology, Cornell University

Bachelor of Arts, Biology, Case Western Reserve University

Affiliations and Professional Service

American Society of Microbiology

American Association for the Advance of Science

Awards and Recognitions

2018-2020 - Management Skills Development Program, PNNL

2017 - Outstanding Performance Award, PNNL

2017 - Nominated for Of the Year (OTY) Award:  PNNL NSD OTY Core Values Award

2014 - Outstanding Performance Award, PNNL

2014 - OTY Award:  PNNL NSD Project Team OTY for DHS Project; Role:  Project Manager, PNNL

2012 - Nominated and selected to attend “Battelle Women in Science and Engineering Symposium” and part of the delegation from PNNL

2010 - OTY Award:  PNNL NSD Project Team OTY Award for DHS Project; Role:  Project Manager, PNNL

2006-2008 - Scientist and Engineer Development Program, PNNL

2000-2001 - State University of New York Fellowship, Cornell University

1997-2000 - National Science Foundation Pre-Doctoral Fellowship, Cornell University



  1. RA Bartholomew and KM Omberg.  “Making Sense of the 2018 National Biodefense Strategy.” Bulletin of the Atomic Scientists.  January 2019. 
  2. KM Omberg, LR Franklin, DR Jackson, KL Taylor, KL Wahl, A Lesperance,  EM Wynkoop, JAS Gray, OP Leiser , SL Frazar, RM Ozanich , and RA Bartholomew. “A Publicly Available Landscape Analysis Tool for Biodefense.” Health Security. February 16(1): 2018.  DOI:  10.1089/hs.2017.0088
  3. Frazar S,  Hund G, Bonheyo GT, Diggans J, Bartholomew RA,  Gehrig L, Greaves M.  2017; Analyzing the Synthetic Biology Supply Chain Reveals Opportunities to Enable Beneficial Research and Deter Unwanted Consequences.” Heath Security. August 15(4).  Online pre-release:  DOI: 10.1089/hs.2016.0083
  4. Bartholomew RA, RM Ozanich, JS Arce, A Heredia-Langer, KH Jarman, HA Colburn, BA Hofstad, JR Hutchison, AM Melville, CJ Bruckner-Lea. 2017 “Evaluation of Immunoassays and General Biological Indicator Tests for Bacillus anthracis and Ricin Detection.” Health Security. Feb; 15(1): 81-96.
  5. RM Ozanich, Colburn HA, KV Victry, RA Bartholomew,  JS Arce, A Heredia-Langer, KH Jarman, HK Kreuzer, CJ Bruckner-Lea. 2017. “Evaluation of PCR instruments For Field Detection of Bacillus anthracis.” Health Security. Feb; 15(1): 70-80.
  6. RA Bartholomew, J Hutchison, T Straub, D Call. 2016. PCR, Real-Time PCR, Digital PCR, and Isothermal Amplification, p 2.3.2-1-2.3.2-13. In Yates M, Nakatsu C, Miller R, Pillai S (ed), Manual of Environmental Microbiology, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818821.ch2.3.2
  7. T Straub T, D Call, CJ Bruckner-Lea, H Colburn, C Baird, RA Bartholomew, RM Ozanich, K Jarman. 2016. Field Application of Pathogen Detection Technologies, p 2.3.4-1-2.3.4-17. In Yates M, Nakatsu C, Miller R, Pillai S (ed), Manual of Environmental Microbiology, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818821.ch2.3.4
  8. Bartholomew RA, Ozanich RM, Bruckner-Lea CJ.  2015 “Technology Development and Science Based Solutions” DomPrep.
  9. Ozanich RM, CJ Bruckner-Lea, RA Bartholomew. 2015.  "National Laboratory Support for First Responders’ Biodetection Needs" DomPrep.