PNNL

Nick Reichart earned his PhD in biochemistry from Montana State University - Bozeman where he developed a workflow for bioorthogonal non-canonical amino acid tagging to determine the activity of uncultured microbes in the hot springs of Yellowstone National Park. Afterward, he started as a postdoctoral research associate in the Chemical Biology team at Pacific Northwest National Laboratory in 2021.

As of 2024, Reichart serves as a biologist on the Cell Signaling and Communications team. His current research utilizes activity-based approaches with fluorescence-activated cell sorting coupled with downstream multi-omics to describe the environmental cues and determinants of various microbial phenotypes. In addition to this, Nick is working to adapt single-cell transcriptomics to various bacteria taxa to decipher cell-to-cell heterogeneities in bioprocessing applications.

• Activity-based protein profiling
• Amplicon sequencing
• Bioinformatics
• Bioorthogonal non-canonical amino acid tagging
• Fluorescence-activated cell sorting
• Microbial physiology
• Multi-omic data analysis

• PhD in biochemistry, Montana State University - Bozeman, 2021
• BS in biology/biological sciences, York College of Pennsylvania, 2014

2023

• Pavlopoulos G.A., F.A. Baltoumas, S. Liu, O. Selvitopi, A. Camargo, S. Nayfach, and A. Azad, et al. 2023. "Unraveling the functional dark matter through global metagenomics." Nature 622, no. 7983:594-602. PNNL-SA-194384. doi:10.1038/s41586-023-06583-7

2022

• Reichart NJ, Bowers RM, Woyke T, Hatzenpichler R. “Metagenomes and metagenome-assembled genomes from substrate-amended hot spring sediment incubations from Yellowstone National Park.” 2022. Microbiology Resource Announcements 11(4), 10-12. doi: 10.1128/mra.01065-21 .

2021

• Krukenberg V, Reichart NJ, Spietz RL, Hatzenpichler R. “Microbial community response to polysaccharide amendment in anoxic hydrothermal sediments of the Guaymas Basin.” 2021. Front. Microbiol. 12:763971. doi: 10.3389/fmicb.2021.763971.
   
• Reichart NJ, Bowers RM, Woyke T, and Hatzenpichler R. “High potential for biomass-degrading enzymes revealed by hot spring metagenomics.” 2021. Front. Microbiol. 12:668238. doi: 10.3389/fmicb.2021.668238.

2020

• Reichart, N. J., Jay, Z. J., Krukenberg, V., Parker, A. E., Spietz, R. L., Hatzenpichler, R. “Activity-based cell sorting reveals responses of uncultured archaea and bacteria to substrate amendment.” 2020. ISMEJ 14, 2851-2861. doi.org/10.1038/s41396-020-00749-1