Chemist
Chemist

Biography

Heather Olson is a chemist with the Biomolecular Pathways team in PNNL’s Environmental Molecular Sciences Division and the Environmental Molecular Sciences Laboratory (EMSL) user program. She performs sample preparation and sample management for a wide variety of sample types, including bacteria (biosafety levels 1 and 2), viruses, soils, plants, tissues (mammalian), algae, and fungi. She specializes in performing multi-omics preparations from challenging sample types, such as working with extremely small masses of protein, extracting protein from highly complex matrices, and overcoming sample contamination issues. Olson’s analytical chemistry background allows her to work with large numbers of samples, as well as extremely complex preparation techniques. She also has experience with the use and maintenance of high-performance liquid chromatography (HPLC), gas chromatography (GC), GC-mass spectrometry (MS), and HPLC-MS instrumentation which is often used to make sure the analytical HPLCs used in the preparation laboratory are working properly. 

Before joining EMSL, Olson worked with the Toxicokinetic and Bioanalytical Chemistry group at PNNL and a geochemistry and analytical chemistry laboratory in Spokane, Washington. This background enables her to provide high-quality and innovative sample preparation methods to further the scientific goals of the principal investigators and collaborators she works with daily. 

Research Interest

  • Pathogens of all types 

  • Soil/rhizosphere microbiomes 

  • New and challenging sample preparations 

Education

  • BS in Biotechnology, Eastern Washington University 

Affiliations and Professional Service

  • Association of Biomolecular Resource Facilities

Publications

2020 

  • Brewer, H., J. Wang, M. Gaffrey, T. Zhang, W. J. Qian, and C. Ansong. 2020. “E-Cigarette Vapor - Understanding Pulmonary Response on the Molecular Level.” Journal of Biomolecular Techniques : JBT 31: S24-S25. http://jbt.abrf.org/jbt-static/index.cfm/page/archives/jbt_archive.htm. 

  • Daly, P., M. Peng, H. D. Mitchell, Y. M. Kim, C. Ansong, H. Brewer, P. de Gijsel, M. S. Lipton, L. M. Markillie, C. D. Nicora, G. Orr, A. Wiebenga, K. S. Hildén, M. A. Kabel, S. E. Baker, M. R. Mäkelä, and R. P. de Vries. 2020. “Colonies of the Fungus Aspergillus niger Are Highly Differentiated to Adapt to Local Carbon Source Variation.” Environmental Microbiology 22 (3): 1154-1166. doi.org/10.1111/1462-2920.14907. 

  • Deatherage Kaiser, B. L., J. M. Jacobs, A. A. Schepmoes, H. M. Brewer, B. J. M. Webb-Robertson, S. Valtier, V. S. Bebarta, and J. N. Adkins. 2020. “Assessment of the Utility of the Oral Fluid and Plasma Proteomes for Hydrocodone Exposure.” Journal of Medical Toxicology 16 (1): 49-60. doi.org/10.1007/s13181-019-00731-0. 

  • Hagen, L. H., C. G. Brooke, C. A. Shaw, A. D. Norbeck, H. Piao, M. Ø. Arntzen, H. M. Olson, A. Copeland, N. Isern, A. Shukla, S. Roux, V. Lombard, B. Henrissat, M. A. O’Malley, I. V. Grigoriev, S. G. Tringe, R. I. Mackie, L. Pasa-Tolic, P. B. Pope, and M. Hess. 2020. “Proteome Specialization of Anaerobic Fungi During Ruminal Degradation of Recalcitrant Plant Fiber.” ISME Journal. doi.org/10.1038/s41396-020-00769-x. 

  • Howard-Varona, C., M. M. Lindback, G. E. Bastien, N. Solonenko, A. A. Zayed, H. B. Jang, B. Andreopoulos, H. M. Brewer, T. Glavina del Rio, J. N. Adkins, S. Paul, M. B. Sullivan, and M. B. Duhaime. 2020. “Phage-Specific Metabolic Reprogramming of Virocells.” ISME Journal 14 (4): 881-895. doi.org/10.1038/s41396-019-0580-z. 

  • Ilhan, Z. E., J. K. DiBaise, S. E. Dautel, N. G. Isern, Y. M. Kim, D. W. Hoyt, A. A. Schepmoes, H. M. Brewer, K. K. Weitz, T. O. Metz, M. D. Crowell, D. W. Kang, B. E. Rittmann, and R. Krajmalnik-Brown. 2020. “Temporospatial Shifts in the Human Gut Microbiome and Metabolome after Gastric Bypass Surgery.” npj Biofilms and Microbiomes 6 (1). doi.org/10.1038/s41522-020-0122-5. 

  • Ogden, A. J., J. J. Bhatt, H. M. Brewer, J. Kintigh, S. M. Kariuki, S. Rudrabhatla, J. N. Adkins, and W. R. Curtis. 2020. “Phloem Exudate Protein Profiles During Drought and Recovery Reveal Abiotic Stress Responses in Tomato Vasculature.” International Journal of Molecular Sciences 21 (12): 1-20. doi.org/10.3390/ijms21124461. 

2019 

  • Christensen G.A., C.M. Gionfriddo, A.J. King, J.G. Moberly, C. Miller, A.C. Somenahally, and S.J. Callister, et al. 2019. "Determining the Reliability of Measuring Mercury Cycling Gene Abundance with Correlations with Mercury and Methylmercury Concentrations." Environmental Science & Technology 53, no. 15:8649-8663. PNNL-SA-145153. doi:10.1021/acs.est.8b06389 

  • Johnston G.P., B. Bradel-Tretheway, P.D. Piehowski, H.M. Brewer, B. Lee, N.T. Usher, and J.L. Zamora, et al. 2019. "Nipah virus-like particle egress is modulated by both cytoskeletal and vesicular trafficking pathways: a validated particle proteomics analysis." mSystems 4, no. 5:Article Number e00194-19. PNNL-SA-142825. doi:10.1128/mSystems.00194-19 

  • Tfaily M., R.M. Wilson, H.M. Brewer, R.K. Chu, H.M. Heyman, D.W. Hoyt, and J.E. Kyle, et al. 2019. "Single-throughput complementary high-resolution analytical techniques for characterizing complex natural organic matter mixtures." Journal of Visualized Experiments. PNNL-SA-138197. doi:10.3791/59035 

2018 

  • Howard-Varona C., K.R. Hargreaves, N.E. Solonenko, L. Markillie, R.A. White, H.M. Brewer, and C.K. Ansong, et al. 2018. "Multiple mechanisms drive phage infection efficiency in nearly-identical hosts." The ISME Journal 12, no. 2018:1605-1618. PNNL-SA-132683. doi:10.1038/s41396-018-0099-8 

  • Naas A., L. Solden, A.D. Norbeck, H.M. Brewer, L. Hagen, I. Heggenes, and A. Mchardy, et al. 2018. “’Candidatus Paraporphyromonas polyenzymogenes’ encodes multi-modular cellulases linked to the type IX secretion system." Microbiome 6. PNNL-SA-129531. doi:10.1186/s40168-018-0421-8 

2017 

  • Dhondt I., V.A. Petyuk, S. Bauer, H.M. Brewer, R.D. Smith, G.G. Depuydt, and B.P. Braeckman. 2017. "Changes of Protein Turnover in Aging Caenorhabditis elegans." Molecular & Cellular Proteomics. MCP 16, no. 9:1621-1633. PNNL-SA-129634. doi:10.1074/mcp.RA117.000049 

  • Haitjema C., S.P. Gilmore, J.K. Henske, K.V. Solomon, R.D. Groot, A. Kuo, and S.J. Mondo, et al. 2017. "A Parts List for Fungal Cellulosomes Revealed by Comparative Genomics." Nature Microbiology 2. PNNL-SA-120227. doi:10.1038/nmicrobiol.2017.87 

  • Hawely A.K., M. Torres Beltran, E. Zaikova, D.A. Walsh, A. Mueller, M. Scofield, and S. Kheirandish, et al. 2017. "A compendium of multi-omic sequence information from the Saanich Inlet water column." Scientific Data 4. PNNL-SA-121885. doi:10.1038/sdata.2017.160 

  • Ledee D.R., M.A. Kang, M. Kajimoto, S.O. Purvine, H.M. Brewer, L. Pasa Tolic, and M.A. Portman. 2017. "Quantitative cardiac phosphoproteomics profiling during ischemia-reperfusion in an immature swine model." American Journal of Physiology. Heart and Circulatory Physiology 313, no. 1:H125-H137. PNNL-SA-128530. doi:10.1152/ajpheart.00842.2016 

  • Nie S., T. Shi, T.L. Fillmore, A.A. Schepmoes, H.M. Brewer, Y. Gao, and E. Song, et al. 2017. "Deep-dive Targeted Quantification for Ultrasensitive Analysis of Proteins in Nondepleted Human Blood Plasma/Serum and Tissues." Analytical Chemistry 89, no. 17:9139-9146. PNNL-SA-126297. doi:10.1021/acs.analchem.7b01878 

  • Staley C., A.P. Ferrieri, M.M. Tfaily, Y. Cui, R.K. Chu, P. Wang, and J.B. Shaw, et al. 2017. "Diurnal Cycling of Rhizosphere Bacterial Communities is Associated with Shifts in Carbon Metabolism." Microbiome 5. PNNL-SA-121718. doi:10.1186/s40168-017-0287-1 

2016 

  • Solomon K.V., C. Haitjema, J.K. Henske, S.P. Gilmore, D. Borges-Rivera, A. Lipzen, and H.M. Brewer, et al. 2016. "Early-branching Gut Fungi Possess A Large, And Comprehensive Array Of Biomass-Degrading Enzymes." Science 351, no. 6278:1192-1195. PNNL-SA-115073. doi:10.1126/science.aad1431 

2015 

  • Elfenbein J.R., L.A. Knodler, E.S. Nakayasu, C. Ansong, H.M. Brewer, L. Bogomolnaya, and L.G. Adams, et al. 2015. "Multicopy single-stranded DNA directs intestinal colonization of enteric pathogens." PLoS Genetics 11, no. 9:e1005472. PNNL-SA-112635. doi:10.1371/journal.pgen.1005472 

2014 

  • Brown J.N., H.M. Brewer, C.D. Nicora, K.K. Weitz, M.J. Morris, A.J. Skabelund, and J.N. Adkins, et al. 2014. "Protein and microRNA biomarkers from lavage, urine, and serum in military personnel evaluated for dyspnea." BMC Medical Genomics 7. PNWD-SA-10347. doi:10.1186/1755-8794-7-58 

  • Depuydt G.G., F. Xie, V.A. Petyuk, A. Smolders, H.M. Brewer, D.G. Camp, and R.D. Smith, et al. 2014. "LC-MS Proteomics Analysis of the Insulin/IGF-1-Deficient Caenorhabditis elegans daf-2(e1370) Mutant Reveals Extensive Restructuring of Intermediary Metabolism." Journal of Proteome Research 13, no. 4:1938-1956. PNNL-SA-86677. doi:10.1021/pr401081b 

  • Hawley A.K., H.M. Brewer, A.D. Norbeck, L. Pasa-Tolic, and S.J. Hallam. 2014. "Metaproteomics reveals differential modes of metabolic coupling among ubiquitous oxygen minimum zone microbes." Proceedings of the National Academy of Sciences of the United States of America 111, no. 31:11395-11400. PNNL-SA-100391. doi:10.1073/pnas.1322132111 

  • Hyung S., P.D. Piehowski, R.J. Moore, D.J. Orton, A.A. Schepmoes, T.R. Clauss, and R.K. Chu, et al. 2014. "Microscale Depletion of High Abundance Proteins in Human Biofluids using IgY14 Immunoaffinity Resin: Analysis of Human Plasma and Cerebrospinal Fluid." Analytical and Bioanalytical Chemistry 406, no. 28:7117-7125. PNNL-SA-92758. doi:10.1007/s00216-014-8058-3 

  • Mohimani H., R. Kersten, W. Liu, M. Wang, S.O. Purvine, S. Wu, and H.M. Brewer, et al. 2014. "Automated genome mining of ribosomal peptide natural products." ACS Chemical Biology 9, no. 7:1545-1551. PNNL-SA-99212. doi:10.1021/cb500199h 

2013 

  • Ansong C., A.C. Rutledge, H.D. Mitchell, S. Chauhan, M.B. Jones, Y. Kim, and K. Mcateer, et al. 2013. "A multi-omic systems approach to elucidating Yersinia virulence mechanisms." Molecular Biosystems 9, no. 1:44-54. PNNL-SA-89164. doi:10.1039/C2MB25287B 

  • Ansong C., S. Wu, D. Meng, X. Liu, H.M. Brewer, B. Kaiser, and E.S. Nakayasu, et al. 2013. "Top-down proteomics reveals a unique protein S-thiolation switch in Salmonella Typimurium in response to infection-like conditions." Proceedings of the National Academy of Sciences of the United States of America 110, no. 25:10153-10158. PNNL-SA-92306. doi:10.1073/pnas.1221210110 

  • Depuydt G.G., F. Xie, V.A. Petyuk, N. Shanmugam, A. Smolders, I. Dhondt, and H.M. Brewer, et al. 2013. "Reduced insulin/Insulin-like Growth Factor-1signaling and dietary restriction inhibit translation but preserve muscle mass in Caenorhabditis elegans." Molecular and Cellular Proteomics 12, no. 12:3624-3639. PNNL-SA-98548. doi:10.1074/mcp.M113.027383 

  • Halim M.F., F. Pfeiffer, J. Zou, A. Frisch, D.H. Haft, S. Wu, and N. Tolic, et al. 2013. "Haloferax volcanii archaeosortase is required for motility, mating, and C-terminal processing of the S-layer glycoprotein." Molecular Microbiology 88, no. 6:1164-1175. PNNL-SA-93808. doi:10.1111/mmi.12248 

  • Hawley A.K., S. Kheirandish, A. Mueller, H.C. Leung, A.D. Norbeck, H.M. Brewer, and L. Pasa-Tolic, et al. 2013. "Molecular Tools for Investigating Microbial Community Structure and Function in Oxygen-Deficient Marine Waters." In Methods in Enzymology: Microbial Metagenomics, Metatranscriptomics, and Metaproteomics, edited by EF DeLong. 305-329. San Diego, California:Academic Press. PNNL-SA-100374. 

  • Kim Y., B. Schmidt, A.S. Kidwai, M.B. Jones, B.L. Deatherage, H.M. Brewer, and H.D. Mitchell, et al. 2013. "Salmonella Modulates Metabolism During Growth under Conditions that Induce Expression of Virulence Genes." Molecular Biosystems 9, no. 6:1522-1534. PNNL-SA-89810. doi:10.1039/C3MB25598K 

2012 

  • Andreev V.P., V.A. Petyuk, H.M. Brewer, Y. Karpievitch, F. Xie, J. Clarke, and D.G. Camp, et al. 2012. "Label-Free Quantitative LC-MS Proteomics of Alzheimer's Disease and Normally Aged Human Brains." Journal of Proteome Research 11, no. 6:3053-3067. PNNL-SA-90452. doi:10.1021/pr3001546 

  • Petrus A.K., K.S. Swithers, C.R. Ranjit, S. Wu, H.M. Brewer, J. Gogarten, and L. Pasa-Tolic, et al. 2012. "Genes for the major structural components of Thermotogales species' togas revealed by proteomic and evolutionary analyses of OmpA and OmpB homologs." PLoS One 7, no. 6:e40236. PNNL-SA-84820. doi:10.1371/journal.pone.0040236 

  • Rutledge A.C., M.B. Jones, S. Chauhan, S.O. Purvine, J. Sanford, M.E. Monroe, and H.M. Brewer, et al. 2012. "Comparative Omics-Driven Genome Annotation Refinement: Application across Yersiniae." PLoS One 7, no. 3:Article No. E33903. PNNL-SA-79961. doi:10.1371/journal.pone.0033903 

2011 

  • Ghazalpour A., B. Bennett, V.A. Petyuk, L. Orazco, R. Hagopian, I. Mungrue, and C.R. Farber, et al. 2011. "Comparative Analysis of Proteome and Transcriptome Variation in Mouse." PLoS Genetics 7, no. 6:Article No. e1001393. PNWD-SA-9360. doi:10.1371/journal.pgen.1001393 

  • Hallam S.J., A.P. Page, L. Constan, Y.C. Song, A.D. Norbeck, H.M. Brewer, and L. Pasa-Tolic. 2011. "Molecular tools for investigating ANME community structure and function." In Methods in Enzymology, Part A, edited by AC Rosenzweig and SW Ragsdale. 75-90. Amsterdam:Elsevier/Academic Press. PNNL-SA-75404. 

  • Lin M.., T. Kikuchi, H.M. Brewer, A.D. Norbeck, and Y. Rikihisa. 2011. "Global proteomic analysis of two tick-borne emerging zoonotic agents: Anaplasma phagocytophilum and Ehrlichia chaffeensis." Frontiers in Microbiology 2. PNNL-SA-77990. doi:10.3389/fmicb.2011.00024 

  • Niemann G., R.N. Brown, J.K. Gustin, A. Stufkens, A.S. Shaikh-Kidwai, J. Li, and J.E. McDermott, et al. 2011. "Discovery of Novel Secreted Virulence Factors from Salmonella enterica Serovar Typhimurium by Proteomic Analysis of Culture Supernatants." Infection and Immunity 79, no. 1:33-43. PNNL-SA-71385. doi:10.1128/IAI.00771-10 

2010 

  • Adkins J.N., H. Mottaz, T.O. Metz, C.K. Ansong, N.P. Manes, R.D. Smith, and F. Heffron. 2010. "Performing Comparative Peptidomics Analyses of Salmonella from Different Growth Conditions." In Peptidomics: Methods and Protocols, Methods in Molecular Biology, edited by Mikhail Soloviev. 13-27. New York, New York:Humana Press, Inc. PNNL-SA-59136. 

  • Brown J.N., R. Estep, D. Lopez-Ferrer, H.M. Brewer, T.R. Clauss, N.P. Manes, and M. O'Connor, et al. 2010. "CHARACTERIZATION OF MACAQUE PULMONARY FLUID PROTEOME DURING MONKEYPOX INFECTION: DYNAMICS OF HOST RESPONSE." Molecular & Cellular Proteomics. MCP 9, no. 12:2760-2771. PNWD-SA-8911. doi:10.1074/mcp.M110.001875 

2009 

  • Ansong C., H. Yoon, S. Porwollik, H. Mottaz-Brewer, B.O. Petritis, N. Jaitly, and J.N. Adkins, et al. 2009. "Global Systems-Level Analysis of Hfq and SmpB Deletion Mutants in Salmonella: Implications for Virulence and Global Protein Translation." PLoS One 4, no. 3:e4809. PNNL-SA-63141. doi:10.1371/journal.pone.0004809 

  • Shi L., C. Ansong, H.S. Smallwood, L.M. Rommereim, J.E. McDermott, H.M. Brewer, and A.D. Norbeck, et al. 2009. "Proteome of Salmonella Enterica SerotypeTyphimurium Grown in a Low Mg2+/pH Medium." Journal of Proteomics and Bioinformatics 2, no. 9:388-397. PNNL-SA-60692. doi:10.4172/jpb.1000099 

  • Shi L., S.M. Chowdhury, H.S. Smallwood, H. Yoon, H.M. Mottaz-Brewer, A.D. Norbeck, and J.E. McDermott, et al. 2009. "Proteomic Investigation of the Time Course Responses of RAW 264.7 Macrophages to Infection with Salmonella enterica." Infection and Immunity 77, no. 8:3227-3233. PNNL-SA-62432. doi:10.1128/IAI.00063-09 

2008 

  • Ansong C., H. Yoon, A.D. Norbeck, J.K. Gustin, J.E. McDermott, H.M. Mottaz, and J. Rue, et al. 2008. "Proteomics Analysis of the Causative Agent of Typhoid Fever." Journal of Proteome Research 7, no. 2:546-557. PNNL-SA-56065. doi:10.1021/pr070434u 

  • Brewer H.M., A.D. Norbeck, J.N. Adkins, N.P. Manes, C. Ansong, L. Shi, and Y. Rikihisa, et al. 2008. "Optimization of proteomic sample preparation procedures for comprehensive protein characterization of pathogenic systems." Journal of Biomolecular Techniques:JBT 19, no. 5:285-295. PNNL-SA-60633. 

  • Elias D.A., S.L. Tollaksen, D.W. Kennedy, H.M. Mottaz, C.S. Giometti, J.S. Mclean, and E.A. Hill, et al. 2008. "The Influence of Cultivation Methods on Shewanella oneidensis Physiology and Proteome Expression." Archives of Microbiology 189, no. 4:313-324. PNNL-SA-57471. doi:10.1007/s00203-007-0321-y 

  • Huang H., M.. Lin, X. Wang, T. Kikuchi, H.M. Mottaz, A.D. Norbeck, and Y. Rikihisa. 2008. "Proteomic Analysis of and Immune Responses to Ehrlichia chaffeensis Lipoproteins." Infection and Immunity 76, no. 8:3405-3414. PNNL-SA-60631. doi:10.1128/IAI.00056-08 

  • Kelly R.T., J.S. Page, R. Zhao, W. Qian, H.M. Mottaz, K. Tang, and R.D. Smith. 2008. "Capillary-Based Multi Nanoelectrospray Emitters: Improvements in Ion Transmission Efficiency and Implementation with Capillary Reversed-Phase LC-ESI-MS." Analytical Chemistry 80, no. 1:143-149. PNNL-SA-56420. doi:10.1021/ac701647s 

  • Manes N.P., R.D. Estep, H.M. Mottaz, R.J. Moore, T.R. Clauss, M.E. Monroe, and X. Du, et al. 2008. "Comparative Proteomics of Human Monkeypox and Vaccinia Intracellular Mature and Extracellular Enveloped Virions." Journal of Proteome Research 3. PNNL-SA-54048. doi:10.1021/pr070432+ 

  • Qian W., D.T. Kaleta, B.A. Ogata, H. Jiang, T. Liu, X. Zhang, and H.M. Mottaz, et al. 2008. "Enhanced Detection of Low Abundant Human Plasma Proteins using a Tandem IgY12-SuperMix Immunoaffinity Separation Strategy." Molecular & Cellular Proteomics. MCP 7, no. 10:1963-1973. PNWD-SA-8062. doi:10.1074/mcp.M800008-MCP200 

  • Shi L., S. Deng, M.J. Marshall, Z. Wang, D.W. Kennedy, A. Dohnalkova, and H.M. Mottaz, et al. 2008. "Direct Involvement of Type II Secretion System in Extracellular Translocation of Shewanella Oneidensis Outer Membrane Cytochromes MtrC and OmcA." Journal of Bacteriology 190, no. 15:5512-5516. PNNL-SA-55117. doi:10.1128/JB.00514-08 

2007 

  • Elias D.A., F. Yang, H.M. Mottaz, A.S. Beliaev, and M.S. Lipton. 2007. "Enrichment of Functional Redox Reactive Proteins and Identification by Mass Spectrometry Results in Several Terminal Fe(III)-reducing Candidate Proteins in Shewanella oneidensis MR-1." Journal of Microbiological Methods 68, no. 2:367-375. PNNL-SA-52214. 

  • Manes N.P., J.K. Gustin, J. Rue, H.M. Mottaz, S.O. Purvine, A.D. Norbeck, and M.E. Monroe, et al. 2007. "Targeted Protein Degradation by Salmonella under Phagosome-Mimicking Culture Conditions Investigated Using Comparative Peptidomics." Molecular & Cellular Proteomics. MCP 6, no. 4:717-727. PNNL-SA-50929. doi:10.1074/mcp.M600282-MCP200 

  • Yang F., N. Jaitly, H. Jayachandran, Q. Lou, M.E. Monroe, X. Du, and M.A. Gritsenko, et al. 2007. "Applying a Targeted Label-free Approach using LC-MS AMT Tags to Evaluate Changes in Protein Phosphorylation Following Phosphatase Inhibition." Journal of Proteome Research 6, no. 11:4489-4497. PNNL-SA-52332. doi:10.1021/pr070068e 

  • Zhang Q., N. Tang, J.W. Brock, H.M. Mottaz, J.M. Ames, J. Baynes, and R.D. Smith, et al. 2007. "Enrichment and Analysis of Nonenzymatically Glycated Peptides: Boronate Affinity Chromatography Coupled with Electron-Transfer Dissociation Mass Spectrometry." Journal of Proteome Research 6, no. 6:2323-2330. PNNL-SA-53989. doi:doi:10.1021/pr070112q 

2006 

  • Adkins J.N., H.M. Mottaz, A.D. Norbeck, J.K. Gustin, J. Rue, T.R. Clauss, and S.O. Purvine, et al. 2006. "Analysis of the Salmonella typhimurium Proteome through Environmental Response toward Infectious Conditions." Molecular & Cellular Proteomics. MCP 5, no. 8:1450-1461. PNNL-SA-46556. doi:10.1074/mcp.M600139-MCP200 

  • Lipton M.S., M.F. Romine, M.E. Monroe, D.A. Elias, L. Pasa-Tolic, G.A. Anderson, and D.J. Anderson, et al. 2006. "AMT Tag Approach to Proteomic Characterization of Deinococcus Radiodurans and Shewanella Oneidensis." In Microbial Proteomics, Methods of Biochemical Analysis, edited by I. Humphery-Smith and M. Hecker. 113-134. Hoboken, New Jersey:John Wiley & Sons, Inc. PNNL-SA-42692. 

  • Liu T., W. Qian, H.M. Mottaz, M.A. Gritsenko, A.D. Norbeck, R.J. Moore, and S.O. Purvine, et al. 2006. "Evaluation of Multi-Protein Immunoaffinity Subtraction for Plasma Proteomics and Candidate Biomarker Discovery Using Mass Spectrometry." Molecular & Cellular Proteomics. MCP 5, no. 11:2167-2174. PNWD-SA-7363. 

  • Shi L., B. Chen, Z. Wang, D.A. Elias, M.U. Mayer, Y.A. Gorby, and S. Ni, et al. 2006. "Isolation of a High-Affinity Functional Protein Complex between OmcA and MtrC: Two Outer Membrane Decaheme c-type Cytochromes of Shewanella oneidensis MR-1." Journal of Bacteriology 188, no. 13:4705-4714. PNNL-SA-45401. doi:10.1128/JB.01966-05 

  • Shi L., J.N. Adkins, J.R. Coleman, A.A. Schepmoes, A. Dohnalkova, H.M. Mottaz, and A.D. Norbeck, et al. 2006. "Proteomic Analysis of Salmonella enterica Serovar Typhimurium Isolated from RAW 264.7 Macrophages: identification of a novel protein that contributes to the replication of serovar Typhimurium inside macrophages." Journal of Biological Chemistry 281. PNNL-SA-51416. doi:10.1074/jbc.M604640200 

  • Simpson D.C., S. Ahn, L. Pasa-Tolic, B. Bogdanov, H.M. Mottaz, A.N. Vilkov, and G.A. Anderson, et al. 2006. "Using size exclusion chromatography-RPLC and RPLC-CIEF as two-dimensional separation strategies for protein profiling." Electrophoresis 27, no. 13:2722-2733. PNNL-SA-46424. doi:10.1002/elps.200600037 

  • Simson D.C., S. Ahn, L. Pasa-Tolic, B. Bogdanov, H.M. Brewer, A.N. Vilkov, and G.A. Anderson, et al. 2006. "Using size exclusion chromatography-RPLC and RPLC-CIEF as two-dimensional separation strategies for protein profiling." Electrophoresis 27, no. 13:2722-2733. PNNL-SA-49244. doi:10.1002/elps.200600037 

  • Wang H.H., W. Qian, M.H. Chin, V.A. Petyuk, R.C. Barry, T. Liu, and M.A. Gritsenko, et al. 2006. "Characterization of the Mouse Brain Proteome Using Global Proteomic Analysis Complemented with Cysteinyl-Peptide Enrichment." Journal of Proteome Research 5, no. 2:361-369. PNNL-SA-46752. 

2005 

  • Luo Q., Y. Shen, K.K. Hixson, R. Zhao, F. Yang, R.J. Moore, and H.M. Mottaz, et al. 2005. "Preparation of 20-µm-i.d. Silica-based Monolithic Columns and Application for Proteomic Analysis." Analytical Chemistry 77, no. 15:5028-5035. PNNL-SA-44543. 

  • Schmid A.K., M.S. Lipton, H.M. Mottaz, M.E. Monroe, R.D. Smith, and M.E. Lidstrom. 2005. "Global whole-cell FTICR mass spectrometric proteomics analysis of the heat shock response in the radioresistant bacterium Deinococcus radiodurans." Journal of Proteome Research 4, no. 3:709-718. PNNL-SA-44177. 

  • Wang H.H., W. Qian, H.M. Mottaz, T.R. Clauss, D.J. Anderson, R.J. Moore, and D.G. Camp, et al. 2005. "Development and Evaluation of a Micro- and Nanoscale Proteomic Sample Preparation Method." Journal of Proteome Research 4, no. 6:2397-2403. PNNL-SA-45347. doi:10.1021/pr050160f 

2004 

  • Jacobs J.M., H.M. Mottaz, L. Yu, D.J. Anderson, R.J. Moore, W.U. Chen, and K.J. Auberry, et al. 2004. "Multidimensional Proteome Analysis of Human Mammary Epithelial Cells." Journal of Proteome Research 3, no. 1:68-75. PNWD-SA-6087. 

  • Prokisch H., C.M. Scharfe, D.G. Camp, W. Xiao, L. David, C. Andreoli, and M.E. Monroe, et al. 2004. "Integrative Analysis of the Mitochondrial Proteome in Yeast." PLoS Biology 2, no. 6:0795-0804. PNWD-SA-6619. 

  • Strittmatter E.F., L.J. Kangas, K. Petritis, H.M. Mottaz, G.A. Anderson, Y. Shen, and J.M. Jacobs, et al. 2004. "Application of Peptide LC Retention Time Information in a Discriminant Function for Peptide Identification by Tandem Mass Spectrometry." Journal of Proteome Research 3, no. 4:760-769. PNNL-SA-40693.