July 28, 2016
Feature

Materials Scientists Make Breakthrough in Biomimetic Coatings

Researchers employ novel approach to assemble peptoids on a solid surface

This microscopy image shows the two-dimensional network of hexagonally-patterned nanoribbons formed by the self-assembly of synthetic protein-like polymers (peptoids) on a mineral surface. Enlarge image.

Results: Nature exquisitely assembles proteins and peptides into highly ordered functional materials, such as those critical for bone formation. These natural materials inspire researchers to innovate approaches to mimic nature for a range of potential biomedical applications. Recently Pacific Northwest National Laboratory (PNNL) -led materials scientists assembled networks of highly ordered synthetic protein-like polymers (peptoids) on a flat surface, marking a major breakthrough in biomimetic coatings.

That research team, led by PNNL's Chun-Long Chen, successfully achieved self-assembly of peptoids into networks of hexagonally patterned nanoribbons on a mineral surfaces. What does that mean to someone who isn't a materials scientist? It means the researchers coated protein-like molecules on solid surfaces in a highly ordered way to create materials similar to hard tissue surfaces, like bones or sea shells. This is similar to how naturally occurring proteins form organized arrays to endow tissues with unique properties. The researchers' novel approach could potentially lead to the development of biomimetic coating materials for a variety of applications.

Why It Matters: Proteins found in Nature form the molecular machines that make life possible. Peptoids are highly stable, protein-like molecules developed by scientists to mimic natural proteins. They are cheap, versatile, and customizable and can be designed to have specific forms and functions. Molecular self-assembly is key in biology to building well-defined protein materials. The researchers were able to achieve a controllable self-assembly of peptoids on a flat surface by manipulating molecular-level interactions through advanced chemistry and microscopy techniques.

Their breakthrough provides a new and robust platform from which to assemble sequence-defined synthetic polymers into biomimetic materials. Resulting applications from the science could include surface coatings with tunable functions for use in bone repair or regeneration, cell adhesion, antifouling, antibacterial activities, and more.

Methods: Chen's research team achieved the controllable assembly of sequence-defined peptoids by manipulating their side-chain chemistry to tune molecular interactions. They then used a special microscope that can see molecules to observe the peptoid assembly in real-time. Researchers also measured how much energy it took to pull the peptoids away from each other and from the mineral surface. The combined results enabled the team to better understand the mechanisms that lead to assembly of these peptoids into hexagonally patterned nanoribbons. They further demonstrated that surface-directed peptoid assembly can be used as a tool to fabricate biomimetic coating materials with controlled functionalities.

What's Next? The team is pushing peptoids to do more through PNNL's Materials Synthesis and Simulation Across Scales Initiative and the U.S. Department of Energy's Office of Basic Energy Sciences' Biomolecular Materials Program. They are studying the ability of peptoids to self-assemble both on solid surfaces and in solution to develop new materials, such as biomimetic membranes. Ultimately, they hope to achieve a more complete understanding of peptoid assembly that is programmable and predictable.

Acknowledgments:

Sponsors: Synthesis and data collection were performed at the Molecular Foundry, Lawrence Berkeley National Laboratory, a Scientific User Facility supported by the U.S. Department of Energy (DOE), Office of Science, Office of Basic Energy Sciences (BES). Data analysis and interpretation were supported by the Materials Synthesis and Simulation Across Scales (MS3) Initiative through Laboratory Directed Research & Development funding at PNNL. Peptoid synthesis and in situ AFM studies were supported by the DOE-BES, Biomolecular Materials Program at PNNL.

User Facility: Molecular Foundry

Research Team: Chun-Long Chen and James J. DeYoreo, PNNL; Ronald N. Zuckermann, Lawrence Berkeley National Laboratory.

Reference: Chen C, R Zuckermann, and JJ De Yoreo. 2016. "Surface-Directed Assembly of Sequence-Defined Synthetic Polymers into Networks of Hexagonally Patterned Nanoribbons with Controlled Functionalities." ACS Nano (In Press). DOI: 10.1021/acsnano.6b01333

Related Highlights: From Seashells to Carbon Dioxide Sealants, Molecular Alchemists at Work

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About PNNL

Pacific Northwest National Laboratory draws on its distinguishing strengths in chemistry, Earth sciences, biology and data science to advance scientific knowledge and address challenges in energy resiliency and national security. Founded in 1965, PNNL is operated by Battelle and supported by the Office of Science of the U.S. Department of Energy. The Office of Science is the single largest supporter of basic research in the physical sciences in the United States and is working to address some of the most pressing challenges of our time. For more information, visit the DOE Office of Science website. For more information on PNNL, visit PNNL's News Center. Follow us on Twitter, Facebook, LinkedIn and Instagram.

Published: July 28, 2016