Hydrothermal liquefaction is a promising technology to upgrade wet organic waste into a biocrude oil for diesel or jet fuel; however, this process generates an acid-rich aqueous phase which poses disposal issues. This hydrothermal liquefaction aqueous phase (HTL-AP) contains organic acids, phenol, and other toxins. This work demonstrates that Y. lipolytica as a unique host to valorize HTL-AP into a variety of co-products. Specifically, strains of Y. lipolytica can tolerate HTL-AP at 10% in defined media and 25% in rich media. The addition of HTL-AP enhances production of the polymer precursor itaconic acid by 3-fold and the polyketide triacetic acid lactone at least 2-fold. Additional co-products (lipids and citric acid) were produced in these fermentations. Finally, bioreactor cultivation enabled 21.6 g/L triacetic acid lactone from 20% HTL-AP in mixed sugar hydrolysate. These results demonstrate the first use of Y. lipolytica in HTL-AP valorization toward production of a portfolio of value-added compounds.
Revised: June 24, 2020 |
Published: October 1, 2020
Citation
Cordova L.T., B.C. Lad, S.A. Ali, A.J. Schmidt, J.M. Billing, K.R. Pomraning, and B.A. Hofstad, et al. 2020.Valorizing a Hydrothermal Liquefaction Aqueous Phase through co-production of chemicals and lipids using the oleaginous yeast Yarrowia lipolytica.Bioresource Technology 313.PNNL-SA-153615.doi:10.1016/j.biortech.2020.123639