Abstract. In the present work, the potential of trapped ion mobility spectrometry
coupled to TOF mass spectrometry (TIMS-TOF MS) for discovery and targeted
monitoring of peptide biomarkers from human-in-mouse xenograft tumor tissue was
evaluated. In particular, a TIMS-MS workflow was developed for the detection and
quantification of peptide biomarkers using internal heavy analogs, taking advantage
of the high mobility resolution (R = 150–250) prior to mass analysis. Five peptide
biomarkers were separated, identified, and quantified using offline nanoESI-TIMSCID-
TOF MS; the results were in good agreement with measurements using a
traditional LC-ESI-MS/MS proteomics workflow. The TIMS-TOF MS analysis permitted
peptide biomarker detection based on accurate mobility, mass measurements,
and high sequence coverage for concentrations in the 10–200 nM range, while simultaneously achieving
discovery measurements
Revised: March 28, 2019 |
Published: May 1, 2018
Citation
Garabedian A., P. Benigni, C. Ramirez, E.M. Baker, T. Liu, R.D. Smith, and F. Fernandez-Lima. 2018.Towards Discovery and Targeted Peptide Biomarker Detection Using nanoESI-TIMS-TOF MS.Journal of the American Society for Mass Spectrometry 29, no. 5:817-826.PNNL-SA-129610.doi:10.1007/s13361-017-1787-8