PNNL

Abstract

As part of the Pacific Northwest National Laboratory’s (PNNL) Science Focus Area program, we are investigating the impact of environmental change on microbial community function in grassland soils. Three grassland soils, representing different moisture regimes, were selected for ultra-deep metagenomic sequencing, resulting in >1 terabase (Tb) of sequence data per location. This dataset serves as a resource for deep analysis of soil microbiome composition and metabolic potential. Soils were collected from three grassland field site locations. Arid regime soil (reliant on irrigated agriculture) was collected from Washington State University’s Irrigated Agriculture Research and Extension Center (IAREC) (46.25 N 119.73 W) and was characterized as a coarse-silty loam. Intermediate precipitation regime soil (rain-fed and irrigated agriculture) was collected from Konza Prairie Biological Station (KPBS) (39.10 N 96.61 W) and was characterized as a fine clay loam. Frequent precipitation regime (rain-fed and tile-drained agriculture) soil was collected from Iowa State University’s Comparison of Biofuel Systems (COBS) (41.92 N 93.75 W) and was characterized as a fine silty-clay loam.