PNNL

Abstract

The 1H, 13C, and 15N chemicals shifts have been extensively assigned for Gl-FKBP, a 109-residue, FKBP-type, peptidyl-proline cis-trans isomerase from Giardia lamblia, an enteric protozoan parasite responsible for giardiasis. These chemical shift assignments were deposited into the BioMagResBank database under the accession number BMRB-17818 and used to determine the solution structure for Gl-FKBP (PDB-ID 2LGO). The core of the Gl-FKBP structure consists of an a-helix (I59 – M69) nestled against the face of a six-strand, antiparallel ß-sheet. The FKBP family of proteins is a potential target class for novel antimicrobials. The chemical shift assignments for Gl-FKBP, in combination with its solution structure (2LGO), will enable backbone dynamics, chemical shift perturbation, and ligand screening studies that will assist employing FKBP-type proteins for antimicrobial drug discovery.