Single-molecule conformational dynamics of fluctuating noncovalent DNA - Protein interactions in a DNA damage recognition | Journal Article

August 22, 2001

Journal Article

Single-molecule conformational dynamics of fluctuating noncovalent DNA - Protein interactions in a DNA damage recognition

Abstract

Single-molecule spectroscopy is a powerful methodology to identify and characterize inhomogeneous samples1-4 because important information may be hidden or lost in conventional ensemble-averaged experiments due to intrinsic sample heterogeneities6-8. Here we report a single-molecule spectroscopy study that reveals fluctuating conformations and molecular interactions within single protein-DNA complexes. This data will facilitate a molecule-level understanding for the dynamic mechanisms of the damage-recognition process in DNA repair9.

Revised: January 12, 2012 | Published: August 22, 2001

Citation

Lu H.P., L.M. Iakoucheva, and E.J. Ackerman. 2001. Single-molecule conformational dynamics of fluctuating noncovalent DNA - Protein interactions in a DNA damage recognition. Journal of the American Chemical Society 123, no. 37:9184-9185. PNWD-SA-5369.