Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS
We report on the quantitative proteomic analysis of single mammalian cells. Fluorescence-activated cell sorting was used to deposit cells into a nanodroplet system for proteomic sample preparation. After processing, samples were analysed by ultrasensitive nanoLC-MS. Using this platform, an average of ~670 protein groups were identified from single HeLa cells and ~330 proteins were quantifiable. We demonstrated the single cell proteomics platform can be used to differentiate cell types from enzyme-dissociated primary cells from human lung and identify specific protein marker among them.
Revised: August 3, 2020 |
Published: September 17, 2018