February 5, 2007
Journal Article

Protein Composition of the Vaccinia Virus Mature Virion

Abstract

The protein content of vaccinia virus mature virions, purified by rate zonal and isopycnic centrifugation and solubilized by SDS or a solution of urea and thiourea, was determined by the accurate mass and time tag technology which uses both tandem mass spectrometry and Fourier transform-ion cyclotron resonance mass spectrometry to detect tryptic peptides separated by high-resolution liquid chromatography. Eighty vaccinia virus-encoded proteins representing 37% of the 218 genes annotated in the complete genome sequence were detected in at least three analyses. Ten proteins accounted for approximately 80% of the mass, while the least abundant proteins made up 1% or less of the mass. Thirteen identified proteins were not previously reported as components of virions. On the other hand, 8 previously described virion proteins were not detected here, presumably due to technical reasons including small size and hydrophobicity. In addition to vaccinia virus-encoded proteins, 24 host proteins omitting isoforms were detected. The most abundant of these were cytoskeletal proteins, heat shock proteins, and proteins involved in translation.

Revised: October 1, 2015 | Published: February 5, 2007

Citation

Resch W., K.K. Hixson, R.J. Moore, M.S. Lipton, and B. Moss. 2007. Protein Composition of the Vaccinia Virus Mature Virion. Virology 358, no. 1:233-247. PNNL-SA-51306.