November 11, 2016
Book Chapter

Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry

Top-down mass spectrometry is a valuable tool for charactering post-translational modifications on histones for understanding of gene control and expression. In this protocol, we describe a top-down workflow using liquid chromatography coupled to mass spectrometry for fast global profiling of changes in histone proteoforms between a wild-type and a mutant of a fungal species. The proteoforms exhibiting different abundances can be subjected to further targeted studies by other mass spectrometric or biochemical assays. This method can be generally adapted for preliminary screening for changes in histone modifications between samples such as wild-type vs. mutant, and control vs. disease.

Revised: April 27, 2017 | Published: November 11, 2016

Zhou M., S. Wu, D.L. Stenoien, Z. Zhang, L. Connolly, M. Freitag, and L. Pasa-Tolic. 2016. "Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry." In Methods in Molecular Biology: Eukaryotic transcriptional and post-transcriptional gene expression regulation, edited by N Wajapeyee and R Gupta. 153-168. New York, New York:Springer. PNNL-SA-113153. doi:10.1007/978-1-4939-6518-2_12