The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several from several organisms were cloned, expressed , purified and evaluated by NMR . Although there was variability among proteomes, overall 20% of these proteins were found to be readily amendable to NMR structure determination. NMR sample preparation was centralized in on facility , a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.
Revised: June 28, 2004 |
Published: February 19, 2002
Citation
Yee A., X. Chang, A. Pineda-Lucena, B. Wu, A. Semsesi, B. Le, and T.A. Ramelot, et al. 2002.An NMR Approach to Structural Proteomics.Proceedings of the National Academy of Sciences of the United States of America 99, no. 4:1825-1830. PNWD-SA-5665.