The hydrosyapatite elution behavior of 3 fragments of human XPA (M98-F219 = XPA-MBD; M59-F219 = XPA-EM; M59-M273 = XPA- N58) and full length human XPA containing a 6-residue, N-terminal histidine tag (h6-XPA) are described. With increasing concentration of potassium phosphate the proteins elute in the order: XPA-EM, XPA-MBD, XPA- N58, and h6-XPA. If hydroxyapatite affinity is related to DNA-binding affinity, then h6-XPA and XPA- N58 may bind DNA more tightly than XPA-MBD and XPA-EM.
Revised: November 2, 2001 |
Published: October 10, 2001
Citation
Buchko G.W., and M.A. Kennedy. 2001.Hydroxyapatite Elution Behavior of Human Nucleotide Excision Repair Protein XPA and Fragments of XPA.Protein and Peptide Letters 8 (5), no. 2001:357-365.PNNL-SA-34760.