Among proposed uses for microarrays in environmental toxiciology is the identification of key contributors to toxicity within a mixture. However, it remains uncertain whether the transcriptomic profiles resulting from exposure to a mixture have patterns of altered gene expression that contain identifiable contributions from each toxicant component. We exposed isogenic rainbow trout Onchorynchus mykiss, to sublethal levels of ethynylestradiol, 2,2,4,4 tetrabromodiphenyl ether, and chromium VI or to a mixture of all three toxicants Fluorescently labeled cDNA were generated and hybridized against a commercially available Salmonid array spotted with 16,000 cDNAs. Data were analyzed using ANOVA (p
Revised: April 4, 2008 |
Published: March 1, 2008
Citation
Hook S.E., A.D. Skillman, B. Gopalan, J.A. Small, and I.R. Schultz. 2008.Gene expression profiles in rainbow trout, Onchorynchus mykiss, exposed to a simple chemical mixture.Toxicological Sciences 102, no. 1:42-60.PNNL-SA-58411.doi:10.1093/toxsci/kfm293