Wood-degrading fungi efficiently decompose wood using a series of steps that are regulated across time and space. RNA sequencing with “bulk” sampling method has indicated processes controlled at the transcription level at different decay stages and locations along hyphal networks. To further understand these regulatory mechanisms, a high-resolution technique for gene expression determination is required. In this work, Fluorescence In Situ Hybridization (FISH) of mRNA was tested and optimized in the ‘brown rot’-type (carbohydrate-selective; lignin-rich residues) model fungus Postia (Rhodonia) placenta to image gene expression at the hyphal scale,
Revised: July 2, 2020 |
Published: September 2, 2019
Citation
Zhang J., D. Hu, G. Orr, and J.S. Schilling. 2019.Fluorescence In Situ mRNA Hybridization for Gene Expression Detection in a Wood Decay Fungus.International Biodeterioration & Biodegradation 143.PNNL-SA-140382.doi:10.1016/j.ibiod.2019.104731