Post-translational modifications can be important biomarkers of function within biological systems. Top-down mass spectrometry is a valuable tool for the characterization of combinatorial modifications on proteins such as histones that have a range of complex modifications. In this chapter, we present a top-down liquid chromatography-mass spectrometry experimental and data analysis workflow for the identification of novel modifications on proteins. The data analysis workflow presented here is reliant on the combination of proteoform searching using TopPIC and data visualization and fragment ion spectral confirmation using LcMsSpectator, part of the Informed-Proteomics package of software.
Published: September 21, 2022
Citation
Wilson J.W., and M. Zhou. 2022.Discovery of unknown posttranslational modifications by top-down mass spectrometry. In Proteoform Identification. Methods in Molecular Biology, edited by L. Sun and X. Liu. 181-199. New York, Ny, New York:Humana.PNNL-SA-158848.doi:10.1007/978-1-0716-2325-1_13