The mineral respiring bacterium Shewanella oneidensis uses a protein complex, MtrCAB, composed of two decaheme cytochromes brought together inside a transmembrane porin to transport electrons across the outer membrane to a variety of mineral-based electron acceptors. A proteoliposome system has been developed that contains methyl viologen (MV) as an internalised electron acceptor and valinomycin (V) as a membrane associated cation exchanger. These proteoliposomes can be used as a model system to investigate MtrCAB function.
Revised: August 12, 2014 |
Published: December 1, 2012
Citation
White G.F., Z. Shi, L. Shi, A. Dohnalkova, J.K. Fredrickson, J.M. Zachara, and J.N. Butt, et al. 2012.Development of a Proteoliposome Model to Probe Transmembrane Electron-Transfer Reactions.Biochemical Society: Transactions 40, no. 6:1257-1260.PNNL-SA-88014.doi:10.1042/BST20120116