January 1, 2002
Conference Paper

Development of A Flexible System for the Simultaneous Conversion of Biomass to Industrial Chemicals and the Production of Industrial Biocatalysts

Abstract

A flexible system was developed for the simultaneous conversion of biomass to industrial chemicals and the production of industrial biocatalysts. In particular, the expression of a bacterial enzyme, beta-glucuronidase (GUS), was investigated using a genetically modified starch-degrading Saccharomyces strain in suspension cultures in starch media. Different sources of starch including corn and waste potato starch were used for yeast biomass accumulation and GUS expression studies under controls of inducible and constitutive promoters. A thermostable bacterial cellulase, Acidothermus cellulolyticus E1 endoglucanase gene was also cloned into an episomal plasmid expression vector and expressed in the starch-degrading Saccharomyces strain.

Revised: April 13, 2004 | Published: January 1, 2002

Citation

Gao J., B.S. Hooker, R.S. Skeen, and D.B. Anderson. 2002. Development of A Flexible System for the Simultaneous Conversion of Biomass to Industrial Chemicals and the Production of Industrial Biocatalysts. In Advancing Sustainability through Green Chemistry and Engineering; ACS SYMPOSIUM SERIES, edited by Lankey, R. L.; Anastas, P. T., 823, 145-162. Washington, District Of Columbia:American Chemical Society. PNNL-SA-31780.