Plant and microbial toxins are considered bioterrorism threat agents because of their extreme toxicity and/or ease of availability. Additionally, some of these toxins are increasingly responsible for accidental food poisonings. The current study utilized an ELISA-based protein antibody microarray for the multiplexed detection of ten biothreat toxins, botulinum neurotoxins (BoNT) A, B, C, D, E, F, ricin, shiga toxins 1 and 2 (Stx), and staphylococcus enterotoxin B (SEB), in buffer and complex biological matrices. The multiplexed assay displayed a sensitivity of 1.3 pg/mL (BoNT/A, BoNT/B, SEB, Stx-1 and Stx-2), 3.3 pg/mL (BoNT/C, BoNT/E, BoNT/F) and 8.2 pg/mL (BoNT/D, ricin). All assays demonstrated high accuracy (75-120 percent recovery) and reproducibility (most coefficients of variation
Revised: January 13, 2015 |
Published: October 21, 2014
Citation
Jenko K., Y. Zhang, Y. Kostenko, Y. Fan, C. Garcia-Rodriguez, J. Lou, and J.D. Marks, et al. 2014.Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins.Analyst 139, no. 20:5093-5102.PNNL-SA-105212.doi:10.1039/c4an01270d