Metabolomics is the quantitative analysis of all metabolites in a given sample. Due to the chemical complexity of the metabolome, optimal separations are required for comprehensive identification and quantification of sample constituents. This chapter provides an overview of both conventional and advanced separations methods in practice for reducing the complexity of metabolite extracts delivered to the mass spectrometer detector, and covers gas chromatography (GC), liquid chromatography (LC), capillary electrophoresis (CE), supercritical fluid chromatography (SFC) and ion mobility spectrometry (IMS) separation techniques coupled with mass spectrometry (MS) as both uni-dimensional and as multi-dimensional approaches.
Revised: September 27, 2016 |
Published: February 16, 2016
Citation
Heyman H.M., X. Zhang, K. Tang, E.S. Baker, and T.O. Metz. 2016.Conventional and Advanced Separations in Mass Spectrometry-Based Metabolomics: Methodologies and Applications. In Reference Module in Chemistry, Molecular Sciences and Chemical Engineering, edited by Reedijk, J. Amsterdam, :Elsevier.PNNL-SA-111253.doi:10.1016/B978-0-12-409547-2.12132-8