August 30, 2019
Journal Article

Consistent Production of Chlorine-Stressed Bacteria from Non-Chlorinated Secondary Sewage Effluents for use in the U.S. Environmental Protection Agency Alternate Test Procedure Protocol

Abstract

The U.S. Environmental Protection Agency Alternative Test Procedure protocol outlines a method to produce chlorine-stressed bacteria for water quality testing. Achieving consistent results is challenging due effluent variability. We describe methods for generating chlorine-stressed total coliforms from secondary effluent to evaluate detection technologies to demonstrate comparability to EPA reference methods

Revised: October 2, 2019 | Published: August 30, 2019

Citation

Hutchison J.R., M.W. Widder, S.M. Brooks, L.M. Brennan, L. Souris, V.T. DeVito, and W.H. van der Schalie, et al. 2019. Consistent Production of Chlorine-Stressed Bacteria from Non-Chlorinated Secondary Sewage Effluents for use in the U.S. Environmental Protection Agency Alternate Test Procedure Protocol. Journal of Microbiological Methods 163. PNNL-SA-142969. doi:10.1016/j.mimet.2019.105651