PNNL

Abstract

Sponges are ancient sessile metazoans that form with their associated microbial symbionts complex functional units (holobionts). They are a rich source of chemical diversity, however, there is limited understanding on which holobiont members produce certain metabolites and how they may contribute to chemical interactions. To address this issue, we performed comparative metabolomic analysis using non-targeted liquid chromatography tandem mass spectrometry (LC-MS/MS) and gas chromatography mass spectrometry (GC-MS) to either whole sponge tissue or fractionated microbial cells across six co-occurring sponge species.