A broad-host-range event detector: expanding and quantifying between Escherichia coli and Pseudomonas species
Modern microbial biodesign relies on the principle that well-characterized genetic parts can be reused and reconfigured for different functions. However, this paradigm has only been successful in a limited set of hosts, mostly comprised from common lab strains of Escherichia coli. It is clear that new applications – such as chemical sensing and event logging in complex environments – will benefit from new host chassis. This study quantitatively compared how a chemical event logger performed across multiple microbial species. An integrase-based sensor and memory device was operated by two representative soil Pseudomonads – Pseudomonas fluorescens SBW25 and Pseudomonas putida DSM 291. Quantitative comparisons were made between these two non-traditional hosts and two bench-mark Escherichia coli chassis including the probiotic Nissle 1917 and common cloning strain DH5a. The performance of sensor and memory components changed according to each host, such that a clear chassis effect was observed and quantified. These results were obtained via fluorescence from reporter proteins that were transcriptionally fused to the integrase and down-stream recombinant region and via data-driven kinetic models. The Pseudomonads proved to be acceptable chassis for the operation of this event logger, which outperformed the common E. coli DH5a in many ways. This study advances an emerging frontier in synthetic biology that aims to build broad-host-range devices and understand the context by which different species can execute programmable genetic operations.
Revised: October 27, 2020 |
Published: February 24, 2020
Khan N., E. Yeung, Y. Farris, S.J. Fansler, and H. Bernstein. 2020.A broad-host-range event detector: expanding and quantifying between Escherichia coli and Pseudomonas species.Synthetic Biology 5, no. 1:Article No: ysaa002.PNNL-SA-148063.doi:10.1093/synbio/ysaa002