In this study, R. opacus PD630, R. jostii RHA1, R. jostii RHA1 VanA-, and their co-culture were employed to convert hydrothermal liquefaction aqueous waste (HTLAW) into lipids. After 11 days, the COD reduction of algal-HTLAW reached 93.4% and 92.7% by R. jostii RHA1 and its mutant VanA-, respectively. Woody-HTLAW promoted lipid accumulation of 0.43 g lipid/g cell dry weight in R. opacus PD630 cells. Additionally, the total number of chemicals in HTLAW decreased by over 1/3 after 7 days of coculture, and 0.10 g/L and 0.46 g/L lipids were incrementally accumulated in the cellular mass during the fermentation of wood- and algal-HTLAW, respectively. The GC-MS data supported that different metabolism pathways were followed when these Rhodococci strains degraded algae- and woody-HTLAW. These results indicated promising potential of bioconversion of under-utilized carbon and toxic compounds in HTLAW into useful products by selected Rhodococci.
Revised: February 25, 2020 |
Published: January 1, 2017
Citation
He Y., X. Li, X. Xue, M.S. Swita, A.J. Schmidt, and B. Yang. 2017.Biological Conversion of the Aqueous Wastes from Hydrothermal Liquefaction of Algae and Pine Wood by Rhodococci.Bioresource Technology 224.PNNL-SA-121581.doi:10.1016/j.biortech.2016.10.059