Cellulose nanocrystal materials have been labeled with modern Alexa Fluor dyes in a process that first links the dye to a cyanuric chloride molecule. Subsequent reaction with cellulose nanocrystals provides dyed solid microcrystalline cellulose material that can be used for bioimaging and suitable for deposition in films and spatially structured microenvironments. It is demonstrated with single molecular fluorescence microscopy that these films are subject to hydrolysis by cellulose enzymes.
Revised: March 31, 2020 |
Published: March 18, 2015
Citation
Grate J.W., K. Mo, Y. Shin, A. Vasdekis, M.G. Warner, R.T. Kelly, and G. Orr, et al. 2015.Alexa Fluor-labeled Fluorescent Cellulose Nanocrystals for Bioimaging Solid Cellulose in Spatially Structured Microenvironments.Bioconjugate Chemistry 26, no. 3:593-601.PNNL-SA-88815.doi:10.1021/acs.bioconjchem.5b00048