The purpose of the collaboration between Agilent Technologies and Battelle is to integrate Battelle’s unique electrospray ionization ion mobility spectrometry (ESI/IMS) technology with Agilent’s state-of-art time of flight mass spectrometry (TOF MS) to design and fabricate multiple ESI/IMS/TOF MS instrument platforms for large scale demonstration of proteomics analyses. The development is aimed at the firm foundation for a premium new IMS/MS product for Agilent and the dissemination of important analytical advances for the scientific community Three initial prototype systems are planned to be utilized as part of this effort. These are as follows: - Prototype 1: This prototype will based upon the integration of Battelle’s existing IMS Version 3 front-end with the latest version of Agilent’s TOF instrument (the SEIKO TOF) that will include an upgraded high-speed ADC. - Prototype 2: This prototype will be based on the integration of Battelle’s more advanced IMS Version 4 front-end with an existing Agilent TOF system already installed at PNNL. - Prototype 3: This prototype will be based on the integration of a modified IMS Version 4 front-end with Agilent’s most advanced TOF instruments. The collaboration consists of four tasks based on the prototypes and these are: - Task 1. Assembly and Testing of Prototype 1 - Task 2. Assembly and Testing of Prototype 2 - Task 3. Fabrication, Assembly, and Testing of Prototype 3 - Task 4. Final report