Activation of nuclear factor kappa B, nitric oxide synthase II, and Tp53 in response of bone marrow stromal cells to the oxidative stress induced by hydrogen peroxide.

November 14, 2000

Journal Article

Activation of nuclear factor kappa B, nitric oxide synthase II, and Tp53 in response of bone marrow stromal cells to the oxidative stress induced by hydrogen peroxide.

Abstract

Reporter gene transfection using a p53(15x)-d2-EGFP construct was performed to generate a D2XRIIGFP24 subline of D2XRII cells that have integrated Tp53 response elements driving green fluorescent protein. Fluorescence imaging of D2XRIIGFP24 cells revealed transactivation of Tp53 reporter associated with H2O2-induced oxidative stress that was modulated by phenyl-N-tert-butylnitrone (PBN). The Tp53 expression induced by H2O2 was dose and time dependent and was substantiated by western blot analysis.

Revised: April 11, 2001 | Published: November 14, 2000

Citation

Gorbunov N.V., K.L. Epperly, W.L. Bigbee, R. Draviam, J.E. Morris, J.S. Greenberger, and B.D. Thrall. 2000. Activation of nuclear factor kappa B, nitric oxide synthase II, and Tp53 in response of bone marrow stromal cells to the oxidative stress induced by hydrogen peroxide. Free Radical Biology & Medicine 29, no. 1:S56. PNWD-SA-5327.