Biomedical Scientist
Biomedical Scientist


Jordan Smith studies the biological fate of chemicals and implications of chemical exposures on human health. He applies advanced molecular tools, cell-based models, in vivo techniques, and computational tools to understand how absorption, distribution, metabolism, and excretion processes affect dosimetry of chemicals and mediate toxicity. His research contributions continue to expand our understanding of life-stage susceptibility to chemicals, non-invasive biomonitoring, target-tissue dosimetry, and biological fate of nanomaterials.


  • PhD in Environmental Toxicology, Texas Tech University
  • BS in Animal Science, University of Idaho
  • BS in Veterinary Science, University of Idaho

Affiliations and Professional Service

  • Joint Appointment, Department of Environmental and Molecular Toxicology, Oregon State University
  • Society of Toxicology (SOT)

Awards and Recognitions

  • Honorable Mention, Best Paper Published, Biological Modeling Specialty Section, SOT, (2017)
  • Top 10 Abstract, Risk Assessment Specialty Section, 2016 SOT Annual Meeting New Orleans, LA, “Extrapolating Salivary Acinar Cell In Vitro Pesticide Transport to Whole Animals Using Computational Modeling” (2016)
  • Best Papers Published Demonstrating Application of Risk Assessment Award, Risk Assessment Specialty Section, SOT, (2013)
  • Best Postdoctoral Publication Award, SOT, (2011)
  • Outstanding Mentor Award, Undergraduate Research Programs, U.S. Department of Energy Office of Science (2010)



  • Smith J.N., K.J. Tyrrell, J.P. Smith, J.P. Smith, K.K. Weitz, and W.D. Faber. 2020. "Linking Internal Dosimetries of the Propyl Metabolic Series in Rats and Humans Using Physiologically Based Pharmacokinetic (PBPK) Modeling." Regulatory Toxicology and Pharmacology 110. PNNL-ACT-SA-10429. doi:10.1016/j.yrtph.2019.104507


  • Han A., C. Timchalk, Z.A. Carver, T.J. Weber, K.J. Tyrrell, R.L. Sontag, and T.M. Gibbins, et al. 2019. "Physiologically based pharmacokinetic modeling of salivary concentrations for noninvasive biomonitoring of 2,4-dichlorophenoxyacetic acid (2,4-D)." Toxicological Sciences 171, no. 2:330-343. PNNL-SA-143610. doi:10.1093/toxsci/kfz206
  • Madeen E.P., L.E. Siddens, S. Uesugi, T.J. McQuistan, R.A. Corley, J.N. Smith, and K.M. Waters, et al. 2019. "Toxicokinetics of Benzo[a]pyrene in Humans: Extensive Metabolism as Determined by UPLC-Accelerator Mass Spectrometry Following Oral Micro-Dosing." Toxicology and Applied Pharmacology 364. PNNL-SA-132492. doi:10.1016/j.taap.2018.12.010
  • Niu X., Q. Shi, W. Zhu, D. Liu, H. Tian, S. Fu, and N. Cheng, et al. 2019. "Unprecedented peroxidase-mimicking activity of single-atom nanozyme with atomically dispersed Fe-Nx moieties hosted by MOF derived porous carbon." Biosensors and Bioelectronics 142. PNNL-SA-145442. doi:10.1016/j.bios.2019.111495


  • Carver Z.A., A. Han, C. Timchalk, T.J. Weber, K.J. Tyrrell, R.L. Sontag, and T. Luders, et al. 2018. "Evaluation of Non-invasive Biomonitoring of 2,4-Dichlorophenoxyacetic Acid (2,4-D) in Saliva." Toxicology 410. PNNL-SA-135310. doi:10.1016/j.tox.2018.08.003
  • Hummel J., E.P. Madeen, B. Siddens, S. Uesugi, T.J. McQuistan, K.A. Anderson, and K. Turteltaub, et al. 2018. "Pharmacokinetics of [14C]-Benzo[a]pyrene (BaP) in Humans: Impact of Co-Administration of Smoked Salmon and BaP Dietary Restriction." Food and Chemical Toxicology 115. PNNL-SA-132485. doi:10.1016/j.fct.2018.03.003
  • Melby E.S., Y. Cui, J. Borgatta, A.C. Mensch, M. Hang, W.B. Chrisler, and A. Dohnalkova, et al. 2018. "Impact of lithiated cobalt oxide and phosphate nanoparticles on rainbow trout gill epithelial cells." Nanotoxicology 12, no. 10:1166-1181. PNNL-SA-137427. doi:10.1080/17435390.2018.1508785
  • Ouyang H., Q. Lu, W. Wang, Y. Song, X. Tu, C. Zhu, and J.N. Smith, et al. 2018. "Dual-readout Immunochromatographic Assay by Utilizing MnO2 Nanoflowers as the Unique Colorimetric/Chemiluminescent Probe." Analytical Chemistry 90, no. 8:5747-5152. PNNL-SA-129235. doi:10.1021/acs.analchem.7b05247
  • Smith J.N., D.G. Thomas, H.D. Jolley, V.K. Kodali, M.H. Littke, P. Munusamy, and D.R. Baer, et al. 2018. "All that is Silver is not Toxic: Silver Ion and Particle Kinetics Reveals the Role of Silver Ion Aging and Dosimetry on the Toxicity of Silver Nanoparticles." Particle and Fibre Toxicology 15. PNNL-SA-135829. doi:10.1186/s12989-018-0283-z
  • Stoddard E.G., R.F. Volk, J.P. Carson, C.M. Ljungberg, T.A. Murphree, J.N. Smith, and N.C. Sadler, et al. 2018. "Multifunctional Activity-Based Protein Profiling of the Developing Lung." Journal of Proteome Research 17, no. 8:2623-2634. PNNL-SA-132239. doi:10.1021/acs.jproteome.8b00086
  • Thomas D.G., J.N. Smith, B.D. Thrall, D.R. Baer, H.D. Jolley, P. Munusamy, and V.K. Kodali, et al. 2018. "ISD3: A Particokinetic Model for Predicting the Combined Effects of Particle Sedimentation, Diffusion and Dissolution on Cellular Dosimetry for In Vitro Systems." Particle and Fibre Toxicology 15. PNNL-SA-125563. doi:10.1186/s12989-018-0243-7


  • Poet T.S., C. Timchalk, M.J. Bartels, J.N. Smith, R. Mcdougal, D. Juberg, and P.S. Price. 2017. "Use of a probabilistic PBPK/PD model to calculate Data Derived Extrapolation Factors for chlorpyrifos." Regulatory Toxicology and Pharmacology 86. PNWD-SA-10561. doi:10.1016/j.yrtph.2017.02.014
  • Smith J.N., D. Mehinagic, S. Nag, S.R. Crowell, and R.A. Corley. 2017. "In vitro metabolism of benzo[a]pyrene-7,8-dihydrodiol and dibenzo[def,p]chrysene-11,12 diol in rodent and human hepatic microsomes." Toxicology Letters 269. PNNL-SA-121283. doi:10.1016/j.toxlet.2017.01.008
  • Smith J.N., K.J. Tyrrell, J.R. Hansen, D.G. Thomas, T.A. Murphree, A.K. Shukla, and T. Luders, et al. 2017. "Plasma Protein Turnover Rates in Rats Using Stable Isotope Labeling, Global Proteomics, and Activity-Based Protein Profiling." Analytical Chemistry 89, no. 24:13559-13566. PNNL-SA-129561. doi:10.1021/acs.analchem.7b03984
  • Smith J.N., Z.A. Carver, T.J. Weber, and C. Timchalk. 2017. "Predicting Transport of 3,5,6-Trichloro-2-pyridinol into Saliva Using a Combination Experimental and Computational Approach." Toxicological Sciences 157, no. 2:438-450. PNNL-SA-123133. doi:10.1093/toxsci/kfx055
  • Stoddard E.G., B.J. Killinger, R.N. Nair, N.C. Sadler, R.F. Volk, S.O. Purvine, and A.K. Shukla, et al. 2017. "Activity-Based Probes for Isoenzyme- and Site-Specific Functional Characterization of Glutathione S- Transferases." Journal of the American Chemical Society 139, no. 45:16032-16035. PNNL-SA-127421. doi:10.1021/jacs.7b07378
  • Timchalk C., T.J. Weber, and J.N. Smith. 2017. "The Need for Non- or Minimally-Invasive Biomonitoring Strategies and the Development of Pharmacokinetic/Pharmacodynamic Models for Quantification." Current Opinion in Toxicology 4. PNNL-SA-124528. doi:10.1016/j.cotox.2017.03.003
  • Wang Y., M.M. Zeinhom, M. Yang, R. Sun, S. Wang, J.N. Smith, and C. Timchalk, et al. 2017. "A 3D-Printed, Portable, Optical-Sensing Platform for Smartphones Capable of detecting the Herbicide 2,4-Dichlorophenoxyacetic Acid." Analytical Chemistry 89, no. 17:9339-9346. PNNL-SA-129156. doi:10.1021/acs.analchem.7b02139


  • Engelhard M.H., J.N. Smith, and D.R. Baer. 2016. "Gold/silver Core-shell 20 nm Nanoparticles Extracted from Citrate Solution Examined by XPS." Surface Science Spectra 23, no. 1:Article No. 29. PNNL-SA-116411. doi:10.1116/1.4953792
  • Sadler N.C., P. Nandhikonda, B.M. Webb-Robertson, C. Ansong, L.N. Anderson, J.N. Smith, and R.A. Corley, et al. 2016. "Hepatic cytochrome P450 activity, abundance, and expression throughout human development." Drug Metabolism and Disposition 44, no. 7:984-91. PNNL-SA-112422. doi:10.1124/dmd.115.068593
  • Zhang X., M.V. Romm, X. Zheng, E.M. Zink, Y. Kim, K.E. Burnum-Johnson, and D.J. Orton, et al. 2016. "SPE-IMS-MS: An automated platform for sub-sixty second surveillance of endogenous metabolites and xenobiotics in bio?uids." Clinical Mass Spectrometry 2. PNNL-SA-117436. doi:10.1016/j.clinms.2016.11.002


  • Mihai C., W.B. Chrisler, Y. Xie, D. Hu, C.J. Szymanski, A. Tolic, and J. Klein, et al. 2015. "Intracellular accumulation dynamics and fate of zinc ions in alveolar epithelial cells exposed to airborne ZnO nanoparticles at the air-liquid interface." Nanotoxicology 9, no. 1:9-22. PNNL-SA-99125. doi:10.3109/17435390.2013.859319
  • Munusamy P., C.M. Wang, M.H. Engelhard, D.R. Baer, J.N. Smith, C. Liu, and V.K. Kodali, et al. 2015. "Comparison of 20 nm silver nanoparticles synthesized with and without a gold core: Structure, dissolution in cell culture media, and biological impact on macrophages." Biointerphases 10, no. 3:031003-1 to 031003-16. PNNL-SA-102846. doi:10.1116/1.4926547
  • Timchalk C., T.J. Weber, and J.N. Smith. 2015. "Computational Strategy for Quantifying Human Pesticide Exposure based upon a Saliva Measurement." Frontiers in Pharmacology 6. PNNL-SA-108384. doi:10.3389/fphar.2015.00115


  • Smith J.N., P.M. Hinderliter, C. Timchalk, M.J. Bartels, and T.S. Poet. 2014. "A Human Life-Stage Physiologically Based Pharmacokinetic and Pharmacodynamic Model for Chlorpyrifos: Development and Validation." Regulatory Toxicology and Pharmacology 69, no. 3:580-597. PNNL-SA-93203. doi:10.1016/j.yrtph.2013.10.005


  • Lanekoff I.T., M. Thomas, J.P. Carson, J.N. Smith, C. Timchalk, and J. Laskin. 2013. "Imaging Nicotine in Rat Brain Tissue by Use of Nanospray Desorption Electrospray Ionization Mass Spectrometry." Analytical Chemistry 85, no. 2:882-889. PNNL-SA-89771. doi:10.1021/ac302308p
  • Smith J.N. 2013. "Challenges for In vitro to in Vivo Extrapolation of Nanomaterial Dosimetry for Human Risk Assessment." Gradient EH&S Nano News 8, no. 3:4. PNNL-SA-99026.
  • Teeguarden J.G., C. Housand, J.N. Smith, P.M. Hinderliter, R. Gunawan, and C. Timchalk. 2013. "A Multi-Route Model of Nicotine-Cotinine Pharmacokinetics, Pharmacodynamics and Brain Nicotinic Acetylcholine Receptor Binding in Humans." Regulatory Toxicology and Pharmacology 65, no. 1:12-28. PNNL-SA-92151. doi:10.1016/J.YRTPH.2012.10.007
  • Zhang W., Y. Tang, D. Du, J.N. Smith, C. Timchalk, D. Liu, and Y. Lin. 2013. "Direct analysis of trichloropyridinol in human saliva using an Au nanoparticles-based immunochromatographic test strip for biomonitoring of exposure to chlorpyrifos." Talanta 114. PNWD-SA-10139. doi:10.1016/j.talanta.2013.06.012


  • Smith J.N., J. Wang, Y. Lin, E.M. Klohe, and C. Timchalk. 2012. "Pharmacokinetics and Pharmacodynamics of Chlorpyrifos and 3,5,6-Trichloro-2-pyridinol in Rat Saliva After Chlorpyrifos Administration." Toxicological Sciences 130, no. 2:245-256. PNWD-SA-9841. doi:10.1093/toxsci/kfs251
  • Timchalk C., J.N. Smith, A.L. Hjerpe, T.S. Poet, and S. Lee. 2012. "Regional Brain Dosimetry for the Organophosphorus Insecticide Chlorpyrifos in the Preweanling Rat." In Parameters for Pesticide QSAR and PBPK/PD Models for Human Risk Assessment. ACS Symposium Series, edited by JB Knaak, C Timchalk and R Tornero-Velez. 195-213. Washington Dc:American Chemical Society. PNWD-SA-9582.


  • Du D., J. Wang, L. Wang, D. Lu, J.N. Smith, C. Timchalk, and Y. Lin. 2011. "Magnetic Electrochemical Sensing Platform for Biomonitoring of Exposure to Organophosphorus Pesticides and Nerve Agents Based on Simultaneous Measurement of Total Enzyme Amount and Enzyme Activity." Analytical Chemistry 83, no. 10:3770-3777. PNNL-SA-78964. doi:10.1021/ac200217d
  • Ellison C.A., J.N. Smith, P.J. Lein, and J.R. Olson. 2011. "Pharmacokinetics and pharmacodynamics of chlorpyrifos in adult male Long-Evans rats following repeated subcutaneous exposure to chlorpyrifos." Toxicology 287, no. 1-3:137-144. PNWD-SA-9469. doi:10.1016/j.tox.2011.06.010
  • Lee S., T.S. Poet, J.N. Smith, A.L. Hjerpe, R. Gunawan, and C. Timchalk. 2011. "IMPACT OF REPEATED NICOTINE AND ALCOHOL COEXPOSURE ON IN VITRO AND IN VIVO CHLORPYRIFOS DOSIMETRY AND CHOLINESTERASE INHIBITION." Journal of Toxicology and Environmental Health Part A 74, no. 20:1334-50. PNWD-SA-9719.
  • Smith J.N., C. Timchalk, M.J. Bartels, and T.S. Poet. 2011. "In Vitro Age-Dependent Enzymatic Metabolism of Chlorpyrifos and Chlorpyrifos-Oxon in Human Hepatic Microsomes and Chlorpyrifos-Oxon in Plasma." Drug Metabolism and Disposition 39, no. 8:1353-1362. PNWD-SA-9231. doi:10.1124/dmd.111.038745
  • Wang L., D. Du, D. Lu, C.T. Lin, J.N. Smith, C. Timchalk, and F. Liu, et al. 2011. "Enzyme-linked immunosorbent assay for detection of organophosphorylated butyrylcholinesterase: A biomarker of exposure to organophosphate agents." Analytica Chimica Acta 693, no. 1-2:1-6. PNNL-SA-78387.
  • Wang L., D. Lu, J. Wang, D. Du, Z. Zou, H. Wang, and J.N. Smith, et al. 2011. "A Novel Immunochromatographic Electrochemical Biosensor for Highly Sensitive and Selective Detection of Trichloropyridinol, a Biomarker of Exposure to Chlorpyrifos." Biosensors and Bioelectronics 26, no. 6:2835-2840. PNWD-SA-9225. doi:10.1016/j.bios.2010.11.008


  • Busby-Hjerpe A.L., J.A. Campbell, J.N. Smith, S. Lee, T.S. Poet, D. Barr, and C. Timchalk. 2010. "Comparative Pharmacokinetics of Chlorpyrifos versus its Major Metabolites Following Oral Administration in the Rat." Toxicology 268, no. 1-2:55-63. PNNL-SA-68197. doi:10.1016/j.tox.2009.11.022
  • Lee S., T.S. Poet, J.N. Smith, A.L. Busby-Hjerpe, and C. Timchalk. 2010. "Effect of In Vivo Nicotine Exposure on Chlorpyrifos Pharmacokinetics and Pharmacodynamics in Rats." Chemico-Biological Interactions 184, no. 3:449-457. PNNL-SA-69195.
  • Lee S., T.S. Poet, J.N. Smith, A.L. Busby-Hjerpe, and C. Timchalk. 2010. "Effect of in vivo nicotine exposure on chlorpyrifos pharmacokinetics and pharmacodynamics in rats." Chemico-Biological Interactions 184, no. 3:449-457. PNNL-SA-74913. doi:10.1016/j.cbi.2010.01.024
  • Smith J.N., J. Wang, Y. Lin, and C. Timchalk. 2010. "Pharmacokinetics of the Chlorpyrifos Metabolite 3,5,6-Trichloro-2-Pyridinol (TCPy) in Rat Saliva." Toxicological Sciences 132, no. 2:315-325. PNWD-SA-8721.
  • Zou Z., D. Du, J. Wang, J.N. Smith, C. Timchalk, Y.Q. Li, and Y. Lin. 2010. "Quantum Dot-Based Immunochromatographic Fluorescent Biosensor for Biomonitoring Trichloropyridinol, a Biomarker of Exposure to Chlorpyrifos." Analytical Chemistry 82, no. 12:5125-5133. PNWD-SA-8896. doi:10.1021/ac100260m


  • Du D., J. Wang, J.N. Smith, C. Timchalk, and Y. Lin. 2009. "Biomonitoring of Organophosphorus Agent Exposure by Reactivation of Cholinesterase Enzyme Based on Carbon Nanotube-Enhanced Flow-Injection Amperometric Detection." Analytical Chemistry 81, no. 22:9314-9320. PNNL-SA-68719.
  • Smith J.N., J.A. Campbell, A.L. Busby-Hjerpe, S. Lee, T.S. Poet, D. Barr, and C. Timchalk. 2009. "Comparative chlorpyrifos pharmacokinetics via multiple routes of exposure and vehicles of administration in the adult rat." Toxicology 261, no. 1-2:47-58. PNWD-SA-8449. doi:10.1016/j.tox.2009.04.041