Dr. Dehong Hu is a Chemist with the Cell Signaling and Communications team in PNNL’s Environmental Molecular Sciences Division and the Environmental Molecular Sciences Laboratory (EMSL) user program. He has more than 20 years of experience in single molecule fluorescence imaging and has coauthored more than 100 publications, including publications in NatureScience, and PNAS.  

Hu works with EMSL users on multiple proposals involving fluorescence in situ hybridization, single-molecule imaging, and single cell analysis. He maintains and develops super resolution microscopes and participates in the U.S. Department of Energy, Office of Science, Biological and Environmental Research program’s bioimaging project. 


  • PhD in Chemistry, University of Minnesota 

  • MS in Chemistry, Peking University 

  • BS in Chemistry, Peking University 

Affiliations and Professional Service

  • American Chemical Society 



Liu W., L. Huang, R.E. Komorek, P. Handakumbura, Y. Zhou, D. Hu, and M.H. Engelhard, et al. 2020. "Correlative Surface Imaging Reveals Chemical Signatures for Bacterial Hotspots on Plant Roots." Analyst 145, no. 2:393-401. PNNL-SA-149225. doi:10.1039/C9AN01954E 

Mao J., Y. Kim, Y. Zhou, D. Hu, C. Zhong, H. Chang, and C.J. Brislawn, et al. 2020. "Genetic and Metabolic Links Between the Murine Microbiome and Memory." Microbiome 8, no. 1:Article No. 53. PNNL-SA-143770. doi:10.1186/s40168-020-00817-w 

Mao, J. H., Y. M. Kim, Y. X. Zhou, D. Hu, C. Zhong, H. Chang, C. J. Brislawn, S. Fansler, S. Langley, Y. Wang, B. Y. L. Peisl, S. E. Celniker, D. W. Threadgill, P. Wilmes, G. Orr, T. O. Metz, J. K. Jansson, and A. M. Snijders. 2020. “Correction To: Genetic and Metabolic Links between the Murine Microbiome and Memory (Microbiome (2020) 8:53 Doi: 10.1186/S40168-020-00817-W).” Microbiome 8 (1).  

Mensch A.C., E.S. Melby, E. Laudadio, I.U. Foreman-Ortiz, Y. Zhang, A. Dohnalkova, and D. Hu, et al. 2020. "Preferential interactions of primary amine-terminated quantum dots with membrane domain boundaries and lipid rafts revealed with nanometer resolution." Environmental Science Nano 7, no. 1:149-161. PNNL-SA-146870. doi:10.1039/C9EN00996E 

Taylor S.E., C.I. Pearce, K.A. Sanguinet, D. Hu, W.B. Chrisler, Y. Kim, and Z. Wang, et al. 2020. "Polystyrene Nano- and Microplastic Accumulation at Arabidopsis and Wheat Root Cap Cells, but No Evidence for Uptake into Roots." Environmental Science: Nano 7, no. 7:1942-1953. PNNL-SA-147042. doi:10.1039/d0en00309c 


Cui Y., E.S. Melby, A.C. Mensch, E. Laudadio, M. Hang, A. Dohnalkova, and D. Hu, et al. 2019. "Quantitative mapping of oxidative stress response to lithium cobalt oxide nanoparticles in single cells using multiplexed in situ gene expression analysis." Nano Letters 19, no. 3:1990-1997. PNNL-SA-140489. doi:10.1021/acs.nanolett.8b05172 

Weber, T. J., W. J. Qian, J. N. Smith, M. A. Gritsenko, D. Hu, W. B. Chrisler, and C. Timchalk. 2019. “Stable Acinar Progenitor Cell Model Identifies Treacle-Dependent Radioresistance.” Radiation Research 192 (2): 135-144.  

Yang X., R. Parashar, N. Sund, A.E. Plymale, T.D. Scheibe, D. Hu, and R.T. Kelly. 2019. "On Modeling Ensemble Transport of Metal Reducing Motile Bacteria." Scientific Reports 9. PNNL-SA-134947. doi:10.1038/s41598-019-51271-0 

Zhang J., D. Hu, G. Orr, and J.S. Schilling. 2019. "Fluorescence In Situ mRNA Hybridization for Gene Expression Detection in a Wood Decay Fungus." International Biodeterioration & Biodegradation 143. PNNL-SA-140382. doi:10.1016/j.ibiod.2019.104731 


Cui, Y., D. Hu, L. M. Markillie, W. B. Chrisler, M. J. Gaffrey, C. Ansong, L. Sussel, and G. Orr. 2018. “Fluctuation Localization Imaging-Based Fluorescence in Situ Hybridization (fliFISH) for Accurate Detection and Counting of RNA Copies in Single Cells.” Nucleic Acids Research 46 (2).  

Jiao S., X. Ren, R. Cao, M.H. Engelhard, Y. Liu, D. Hu, and D. Mei, et al. 2018. "Stable Cycling of High-Voltage Lithium Metal Batteries in Ether Electrolytes." Nature Energy 3, no. 9:739-746. PNNL-SA-131449. doi:10.1038/s41560-018-0199-8 

Li X., J. Tao, D. Hu, M.H. Engelhard, W. Zhao, J. Zhang, and W. Xu. 2018. "Stability of Polymeric Separators in Lithium Metal Batteries in a Low Voltage Environment." Journal of Materials Chemistry A 6, no. 12:5006-5015. PNNL-SA-131076. doi:10.1039/C7TA11259A 

Olenick L.L., J. Troiano, A.M. Vartanian, E.S. Melby, A.C. Mensch, L. Zhang, and J. Hong, et al. 2018. "Lipid Corona Formation from Nanoparticle Interactions with Bilayers." Chem 4, no. 11:2709-2723. PNNL-SA-138886. doi:10.1016/j.chempr.2018.09.018 

Proetto M.T., C.E. Callmann, J.B. Cliff, C.J. Szymanski, D. Hu, S.B. Howell, and J.E. Evans, et al. 2018. "Tumor Retention of Enzyme-Responsive Pt(II) Drug-Loaded Nanoparticles Imaged by Nanoscale Secondary Ion Mass Spectrometry and Fluorescence Microscopy." ACS Central Science 4, no. 11:1477-1484. PNNL-SA-138579. doi:10.1021/acscentsci.8b00444 

Varga T., T.C. Droubay, L. Kovarik, D. Hu, and S.A. Chambers. 2018. "Controlling the structure and ferroic properties of strained epitaxial NiTiO3 thin films on sapphire by post-deposition annealing." Thin Solid Films 662. PNNL-SA-129160. doi:10.1016/j.tsf.2018.07.030 


Duan W., V.S. Vemuri, D. Hu, Z. Yang, and X. Wei. 2017. "A Protocol for Electrochemical Evaluation and State of Charge Diagnostics of A Symmetric Organic Redox Flow Battery." Journal of Visualized Experiments. PNNL-SA-118931. doi:10.3791/55171 

Lemmer K.C., W. Zhang, S.J. Langer, A. Dohnalkova, D. Hu, R.A. Lemke, and J.S. Piotrowski, et al. 2017. "Mutations that alter the bacterial cell envelope increase lipid production." mBio 8, no. 3:Article No. e00513-17. PNNL-SA-125854. doi:10.1128/mBio.00513-17 

Mobberley J.M., S.R. Lindemann, H.C. Bernstein, J.J. Moran, R.S. Renslow, J.T. Babauta, and D. Hu, et al. 2017. "Organismal and spatial partitioning of energy and macronutrient transformations within a hypersaline mat." FEMS Microbiology Ecology 93, no. 4:Article No. fix028. PNNL-SA-124562. doi:10.1093/femsec/fix028 

Novikova I.V., C.R. Smallwood, Y. Gong, D. Hu, L. Hendricks, J.E. Evans, and A. Bhattarai, et al. 2017. "Multimodal Hyperspectral Optical Microscopy." Chemical Physics 498-499. PNNL-SA-120193. doi:10.1016/j.chemphys.2017.08.011 

Varga T., T.C. Droubay, L. Kovarik, M.I. Nandasiri, V. Shutthanandan, D. Hu, and B. Kim, et al. 2017. "Coupled Lattice Polarization and Ferromagnetism in Multiferroic NiTiO3 Thin Films." ACS Applied Materials & Interfaces 9, no. 26:21879-21890. PNNL-SA-121771. doi:10.1021/acsami.7b04481 

Wang Y., Z. Li, M. Liu, D. Hu, Y. Lin, and J. Li. 2017. "Multiple-Targeted Graphene-based Nanocarrier for Intracellular Imaging of mRNAs." Analytica Chimica Acta 983. PNNL-SA-126440. doi:10.1016/j.aca.2017.07.017 


Duan W., V.S. Vemuri, J.D. Milshtein, S. Laramie, R.D. Dmello, J. Huang, and L. Zhang, et al. 2016. "A Symmetric Organic - Based Nonaqueous Redox Flow Battery and Its State of Charge Diagnostics by FTIR." Journal of Materials Chemistry A 4, no. 15:5448-5456. PNNL-SA-115896. doi:10.1039/c6ta01177b 

El-Khoury P.Z., P. Abellan, R.L. Chantry, Y. Gong, A.G. Joly, I.V. Novikova, and J.E. Evans, et al. 2016. "The information content in single-molecule Raman nanoscopy." Advances in Physics: X 1, no. 1:35-54. PNNL-SA-114470. doi:10.1080/23746149.2016.1140010 

Ford N.R., K.A. Hecht, D. Hu, G. Orr, Y. Xiong, T. Squier, and G.L. Rorrer, et al. 2016. "Antigen Binding and Site-Directed Labeling of Biosilica-Immobilized Fusion Proteins Expressed in Diatoms." ACS Synthetic Biology 5, no. 3:193-199. PNNL-SA-113297. doi:10.1021/acssynbio.5b00191 

Melby E.S., A.C. Mensch, S.E. Lohse, D. Hu, G. Orr, C. Murphy, and R.J. Hamers, et al. 2016. "Formation of supported lipid bilayers containing phase-segregated domains and their interaction with gold nanoparticles." Environmental Science: Nano 3, no. 1:45-55. PNNL-SA-111557. doi:10.1039/C5EN00098J 

Mitchell H.D., L. Markillie, W.B. Chrisler, M.J. Gaffrey, D. Hu, C.J. Szymanski, and Y. Xie, et al. 2016. "Cells Respond to Distinct Nanoparticle Properties with Multiple Strategies as Revealed by Single-Cell RNA-Seq." ACS Nano 10, no. 11:10173-10185. PNNL-SA-121323. doi:10.1021/acsnano.6b05452 

Pomraning K.R., Y. Kim, C.D. Nicora, R.K. Chu, E.L. Bredeweg, S.O. Purvine, and D. Hu, et al. 2016. "Multi-omics analysis reveals regulators of the response to nitrogen limitation in Yarrowia lipolytica." BMC Genomics 17, no. 138:1-18. PNNL-SA-112784. doi:10.1186/s12864-016-2471-2 

Proetto M.T., C.R. Anderton, D. Hu, C.J. Szymanski, Z. Zhu, J.P. Patterson, and J.K. Kammeyer, et al. 2016. "Cellular Delivery of Nanoparticles Revealed with Combined Optical and Isotopic Nanoscopy." ACS Nano 10, no. 4:4046-4054. PNNL-SA-106955. doi:10.1021/acsnano.5b06477 

Schlecht W., K. Li, D. Hu, and W. Dong. 2016. "Fluorescence Based Characterization of Calcium Sensitizer Action on the Troponin Complex." Chemical Biology and Drug Design 87, no. 2:171-181. PNNL-SA-106744. doi:10.1111/cbdd.12651 

Zhai Y., J.S. DuChene, Y. Wang, J. Qiu, A.C. Johnson-Peck, B. You, and W. Guo, et al. 2016. "Polyvinylpyrrolidone-induced anisotropic growth of gold nanoprisms in plasmon-driven synthesis." Nature Materials 15, no. 8:889-896. PNNL-SA-107281. doi:10.1038/nmat4683 

Zhang J., G.N. Presley, K.E. Hammel, J.S. Ryu, J. Menke, M. Figueroa, and D. Hu, et al. 2016. "Localizing gene regulation reveals a staggered wood decay mechanism for the brown rot fungus Postia placenta." Proceedings of the National Academy of Sciences of the United States of America 113, no. 39:10968-10973. PNNL-SA-114266. doi:10.1073/pnas.1608454113 

Zhang L., W. Jiang, A.C. Dissanayake, T. Varga, J. Zhang, Z. Zhu, and D. Hu, et al. 2016. "Grain growth of nanocrystalline 3C-SiC under Au ion irradiation at elevated temperatures." Journal of Physics D. Applied Physics 49, no. 3:035304. PNNL-SA-110626. doi:10.1088/0022-3727/49/3/035304 


Chen W., E.W. Rosser, D. Zhang, W. Shi, Y. Li, W. Dong, and H. Ma, et al. 2015. "A Specific Nucleophilic Ring-Opening Reaction of Aziridines as a Unique Platform for the Construction of Hydrogen Polysulfides Sensors." Organic Letters 17, no. 11:2776-2779. PNNL-SA-108754. doi:10.1021/acs.orglett.5b01194 

El-Khoury P.Z., Y. Gong, P. Abellan, B.W. Arey, A.G. Joly, D. Hu, and J.E. Evans, et al. 2015. "Tip-Enhanced Raman Nanographs: Mapping Topography and Local Electric Fields." Nano Letters 15, no. 4:2385-2390. PNNL-SA-106991. doi:10.1021/acs.nanolett.5b00609 

Gong Y., A.G. Joly, D. Hu, P.Z. El-Khoury, and W.P. Hess. 2015. "Ultrafast Imaging of Surface Plasmons Propagating on a Gold Surface." Nano Letters 15, no. 5:3472-3478. PNNL-SA-108438. doi:10.1021/acs.nanolett.5b00803 

Grate J.W., K. Mo, Y. Shin, A. Vasdekis, M.G. Warner, R.T. Kelly, and G. Orr, et al. 2015. "Alexa Fluor-labeled Fluorescent Cellulose Nanocrystals for Bioimaging Solid Cellulose in Spatially Structured Microenvironments." Bioconjugate Chemistry 26, no. 3:593-601. PNNL-SA-88815. doi:10.1021/acs.bioconjchem.5b00048 

Jacobson K.H., I.L. Gunsolus, T.R. Kuech, J.M. Troiano, E.S. Melby, S.E. Lohse, and D. Hu, et al. 2015. "Lipopolysaccharide density and structure govern the extent and distance of nanoparticle interaction with actual and model bacterial outer membranes." Environmental Science & Technology 49, no. 17:10642-10652. PNNL-SA-112154. doi:10.1021/acs.est.5b01841 

Liu T.L., J.T. Cox, D. Hu, X. Deng, J.Z. Hu, M.Y. Hu, and J. Xiao, et al. 2015. "A Fundamental Study on the [(µ-Cl)3Mg2(THF)6]+ Dimer Electrolytes for Rechargeable Mg Batteries." Chemical Communications 51, no. 12:2312-2315. PNNL-SA-104532. doi:10.1039/c4cc07621d 

Lu N., A. Massoudieh, X. Liang, D. Hu, T. Kamai, T.R. Ginn, and J.L. Zilles, et al. 2015. "Swimming motility reduces Azotobacter vinelandii deposition to silica surfaces." Journal of Environmental Quality 44, no. 5:1366-1375. PNNL-SA-103558. doi:10.2134/jeq2015.03.0141 

Mihai C., W.B. Chrisler, Y. Xie, D. Hu, C.J. Szymanski, A. Tolic, and J. Klein, et al. 2015. "Intracellular accumulation dynamics and fate of zinc ions in alveolar epithelial cells exposed to airborne ZnO nanoparticles at the air-liquid interface." Nanotoxicology 9, no. 1:9-22. PNNL-SA-99125. doi:10.3109/17435390.2013.859319 

Szymanski C.J., P. Munusamy, C. Mihai, Y. Xie, D. Hu, M.K. Gilles, and T. Tyliszczak, et al. 2015. "Shifts in oxidation states of cerium oxide nanoparticles detected inside intact hydrated cells and organelles." Biomaterials 62. PNNL-SA-110620. doi:10.1016/j.biomaterials.2015.05.042 

Troiano J., L.L. Olenick, T.R. Kuech, E.S. Melby, D. Hu, S.E. Lohse, and A.C. Mensch, et al. 2015. "Direct Probes of 4 nm Diameter Gold Nanoparticles Interacting with Supported Lipid Bylayers." Journal of Physical Chemistry C 119, no. 1:534-546. PNNL-SA-102945. doi:10.1021/jp512107z 

Varga T., T.C. Droubay, M.E. Bowden, L. Kovarik, D. Hu, and S.A. Chambers. 2015. "Strain-dependence Of The Structure And Ferroic Properties Of Epitaxial NiTiO3 Thin Films Grown On Different Substrates." Advances in Condensed Matter Physics 2015. PNNL-SA-110090. doi:10.1155/2015/493045 

Varga T., T.C. Droubay, M.E. Bowden, S.A. Stephens, S. Manandhar, V. Shutthanandan, and R.J. Colby, et al. 2015. "Strain-dependence Of The Structure And Ferroic Properties Of Epitaxial Ni-1 (-) Ti-x(1) (-) O-y(3) Thin Films Grown On Sapphire Substrates." Thin Solid Films 578. PNNL-SA-106024. doi:10.1016/j.tsf.2015.02.016 

Wang Z., B. Liu, E. Zhao, K. Jin, Y. Du, J.J. Neeway, and J.V. Ryan, et al. 2015. "Argon Cluster Sputtering Source for ToF-SIMS Depth Profiling of Insulating Materials: High Sputter Rate and Accurate Interfacial Information." Journal of the American Society for Mass Spectrometry 26, no. 8:1283-1290. PNNL-SA-105691. doi:10.1007/s13361-015-1159-1 

Zhang, L., W. Jiang, A. Dissanayake, T. Varga, J. Zhang, Z. Zhu, D. Hu, H. Wang, C. H. Henager, and T. Wang. 2015. “Grain Growth of Nanocrystalline 3C-SiC under Au Ion Irradiation at Elevated Temperatures.” Journal of Physics D: Applied Physics 49 (3).  


Cole J.K., J.R. Hutchison, R.S. Renslow, Y. Kim, W.B. Chrisler, H.E. Engelmann, and A. Dohnalkova, et al. 2014. "Phototrophic Biofilm Assembly in Microbial-Mat-Derived Unicyanobacterial Consortia: Model Systems for the Study of Autotroph-Heterotroph Interactions." Frontiers in Microbiology 5. PNNL-SA-99657. doi:10.3389/fmicb.2014.00109 

El-Khoury P.Z., E. Khon, Y. Gong, A.G. Joly, P. Abellan, J.E. Evans, and N.D. Browning, et al. 2014. "Electric Field Enhancement in a Self-Assembled 2D Array of Silver Nanospheres." Journal of Chemical Physics 141, no. 21:Article No. 214308. PNNL-SA-105010. doi:10.1063/1.4902905 

El-Khoury P.Z., T.W. Ueltschi, A.L. Mifflin, D. Hu, and W.P. Hess. 2014. "Frequency Resolved Nanoscale Chemical Imaging of 4,4'-Dimercaptostilbene on Silver." Journal of Physical Chemistry C 118, no. 47:27525-27530. PNNL-SA-100249. doi:10.1021/jp509082c 

Gunsolus I.L., D. Hu, C. Mihai, S.E. Lohse, C. Lee, M. Torelli, and R.J. Hamers, et al. 2014. "Facile method to stain the bacterial cell surface for super-resolution fluorescence microscopy." Analyst 139, no. 12:3174-3178. PNNL-SA-102724. doi:10.1039/c4an00574k 


Brown E., D. Hu, N. Abu-Lail, and X. Zhang. 2013. "Potential of nanocrystalline cellulose-fibrin nanocomposites for artificial vascular graft applications." Biomacromolecules 14, no. 4:1063-1071. PNNL-SA-89375. 

Chien M., A.S. Carlini, D. Hu, C.V. Barback, A.M. Rush, D.J. Hall, and G. Orr, et al. 2013. "Enzyme-Directed Assembly of Nanoparticles in Tumors Monitored by In Vivo Whole Animal and Ex Vivo Super-Resolution Fluorescence Imaging." Journal of the American Chemical Society 135, no. 50:18710-18713. PNNL-SA-98263. doi:10.1021/ja408182p 

El-Khoury P.Z., D. Hu, and W.P. Hess. 2013. "Junction Plasmon-Induced Molecular Reorientation." Journal of Physical Chemistry Letters 4, no. 20:3435-3439. PNNL-SA-97929. doi:10.1021/jz401838b 

El-Khoury P.Z., D. Hu, V. Apkarian, and W.P. Hess. 2013. "Raman Scattering at Plasmonic Junctions Shorted by Conductive Molecular Bridges." Nano Letters 13, no. 4:1858-1861. PNNL-SA-93981. doi:10.1021/nl400733r 

El-Khoury P.Z., S.J. Peppernick, D. Hu, A.G. Joly, and W.P. Hess. 2013. "The Origin of Surface-Enhanced Raman Scattering of 4,4' -Biphenyldicarboxylate on Silver Substrates." Journal of Physical Chemistry C 117, no. 14:7260-7268. PNNL-SA-92092. doi:10.1021/jp401026x 

Geng H., S. Pan, and D. Hu. 2013. "Electrogenerated Chemiluminescence and Fluorescence Lifetime Spatial Heterogeneity of Poly (2-methoxy-5-(2-ethylhexyloxy)-1, 4-phenylenevinylene) in Presence of [6,6]-phenyl-C61-butyric Acid Methyl Ester." In MRS Proceedings: Symposium E/H - Photovoltaic Technologies, Devices and Systems Based on Inorganic Materials, Small Organic Molecules and Hybrids, 1493, Paper No. mrsf12-1493-h01-09. Warrendale, Pennsylvania:Materials Research Society. PNNL-SA-91989. doi:10.1557/opl.2013.19 

Hu D., B. Zhao, Y. Xie, G. Orr, and A.D. Li. 2013. "Understanding Super-Resolution Nanoscopy and Its Biological Applications in Cell Imaging." Physical Chemistry Chemical Physics. PCCP 15, no. 36:14856-14861. PNNL-SA-95464. doi:10.1039/C3CP51629F 

Liu Y., C. Zhang, D. Hu, M.S. Kuhlenschmidt, T.B. Kuhlenschmidt, S.E. Mylon, and R. Kong, et al. 2013. "Role of collector alternating charged patches on transport of Cryptosporidium parvum oocyst in a patchwise charged heterogeneous micromodel." Environmental Science & Technology 47, no. 6:2670-2678. PNNL-SA-91345. doi:10.1021/es304075j 

Nasybulin E.N., W. Xu, M.H. Engelhard, X.S. Li, M. Gu, D. Hu, and J. Zhang. 2013. "Electrocatalytic properties of poly(3,4-ethylenedioxythiophene) (PEDOT) in Li-O2 battery." Electrochemistry Communications 29. PNNL-SA-92140. doi:10.1016/j.elecom.2013.01.011 

Varga T., T.C. Droubay, M.E. Bowden, R.J. Colby, S. Manandhar, V. Shutthanandan, and D. Hu, et al. 2013. "Coexistence of Weak Ferromagnetism and Polar Lattice Distortion in Epitaxial NiTiO3 thin films of the LiNbO3-Type Structure." Journal of Vacuum Science and Technology B--Microelectronics and Nanometer Structures 31, no. 3:Article No. 030603. PNNL-SA-92833. doi:10.1116/1.4801664 

Wang Y., Z. Li, T.J. Weber, D. Hu, C.T. Lin, J. Li, and Y. Lin. 2013. "In Situ Live Cell Sensing of Multiple Nucleotides Exploiting DNA/RNA Aptamers and Graphene Oxide Nanosheets." Analytical Chemistry 85, no. 14:6775-6782. PNNL-SA-96634. doi:10.1021/ac400858g 


Chen X., W. Xu, J. Xiao, M.H. Engelhard, F. Ding, D. Mei, and D. Hu, et al. 2012. "Effects of cell positive cans and separators on the performance of high-voltage Li-ion batteries." Journal of Power Sources 213. PNNL-SA-84951. doi:10.1016/j.jpowsour.2012.04.009 

Xu, W., A. Read, P. K. Koech, D. Hu, C. Wang, J. Xiao, A. B. Padmaperuma, G. L. Graff, J. Liu, and J. G. Zhang. 2012. “Factors Affecting the Battery Performance of Anthraquinone-Based Organic Cathode Materials.” Journal of Materials Chemistry 22 (9): 4032-4039.  

Clayton D.A., T. McPherson, S. Pan, M. Chen, D.A. Dixon, and D. Hu. 2012. "Spatial and temporal variation of surface-enhanced Raman scattering at Ag nanowires in aqueous solution." Physical Chemistry Chemical Physics. PCCP 15, no. 3:850-859. PNNL-SA-82982. 

Jin H., Y. Yu, W.B. Chrisler, Y. Xiong, D. Hu, and C. Lei. 2012. "Delivery of MicroR A-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing." Breast Cancer: Basic and Clinical Research 6. PNWD-SA-9642. doi:10.4137/BCBCR.S8513 

Xu W., A.L. Read, P.K. Koech, D. Hu, C.M. Wang, J. Xiao, and A.B. Padmaperuma, et al. 2012. "Factors Affecting the Battery Performance of Anthraquinone-based Organic Cathode Materials." Journal of Materials Chemistry 22, no. 9:4032-4039. PNNL-SA-83987. doi:10.1039/c2jm15764k 


Li A.D., C. Zhan, D. Hu, W. Wan, and J. Yao. 2011. "Photoswitchable Nanoprobes Offer Unlimited Brightness in Frequency-Domain Imaging." Journal of the American Chemical Society 133, no. 20:7628-7631. PNNL-SA-75462. doi:10.1021/ja1108479 

Tian Z., Z. Tian, A.D. Li, and D. Hu. 2011. "Super-Resolution Fluorescence Nanoscopy Applied to Image Core-Shell Photoswitching Nanoparticles and their Self-Assemblies." Chemical Communications 47, no. 4:1258-1260. PNNL-SA-71515. doi:10.1039/C0CC03217D 

Xiao J., J.Z. Hu, D. Wang, D. Hu, W. Xu, G.L. Graff, and Z. Nie, et al. 2011. "Investigation of the Rechargeability of Li-O2 Batteries in Non-aqueous Electrolyte." Journal of Power Sources 196, no. 13:5674-5678. PNNL-SA-75791. 

Xu W., K. Xu, V.V. Viswanathan, S.A. Towne, J.S. Hardy, J. Xiao, and Z. Nie, et al. 2011. "Reaction Mechanisms for the Limited Reversibility of Li-O2 Chemistry in Organic Carbonate Electrolytes." Journal of Power Sources 196, no. 22:9631-9639. PNNL-SA-78429. doi:10.1016/j.jpowsour.2011.06.099 

Xu W., V.V. Viswanathan, D. Wang, S.A. Towne, J. Xiao, Z. Nie, and D. Hu, et al. 2011. "Investigation on the Charging Process of Li2O2-Based Air Electrodes in Li-O2 Batteries with Organic Carbonate Electrolytes." Journal of Power Sources 196, no. 8:3894-3899. PNNL-SA-75700. 


Hu D., and G. Orr. 2010. "Nanometer Resolution Imaging by SIngle Molecule Switching." Nano Reviews 1. PNNL-SA-70811. doi:10.3402/nano.v1i0.5122 

Li G., D. Hu, G. Xia, and Z.C. Zhang. 2010. "Catalyst Structure-Performance Relationship Identified by High-Throughput Operando Method: New Insight for Silica-Supported Vanadium Oxide for Methanol Oxidation." Topics in Catalysis 53, no. 1-2:40-48. PNNL-SA-58799. 

Wang D., R. Kou, D. Choi, Z. Yang, Z. Nie, J. Li, and L.V. Saraf, et al. 2010. "Ternary Self-Assembly of Ordered Metal Oxide-Graphene Nanocomposites for Electrochemical Energy Storage." ACS Nano 4, no. 3:1587-1595. PNNL-SA-70609. doi:10.1021/nn901819n 

Wang Y., Z. Li, D. Hu, C.T. Lin, J. Li, and Y. Lin. 2010. "Aptamer/Graphene Oxide Nanocomplex for In Situ Molecular Probing in Living Cells." Journal of the American Chemical Society 132, no. 27:9274-9276. PNNL-SA-73086. doi:10.1021/ja103169v 


Hu D., C. Lei, and E.J. Ackerman. 2009. "Single-molecule electron transfer reactions in nanomaterials." In Microscopy and Microanalysis 2009: Proceedings, July 26-30, 2009, Richmond, Virginia, edited by LN Brewer, 15, 1138-1139. New York:Cambridge University Press. PNNL-SA-64503. doi:10.1017/S1431927609095178 

Hu D., Z. Tian, Z. Tian, W. Wu, W. Wan, and A.D. Li. 2009. "Single-Molecule Photoswitching Enables High-Resolution Optical Imaging." In Microscopy and Microanalysis, 15, 840-841. Cambridge:Cambridge University Press. PNNL-SA-64857. 

Lei C., D. Hu, and E.J. Ackerman. 2009. "Clay Nanoparticle-Supported Single-Molecule Fluorescence Spectroelectrochemistry." Nano Letters 9, no. 2:655-658. PNNL-SA-61456. 

Li G., D. Hu, G. Xia, and Z.C. Zhang. 2009. "Methanol Partial Oxidation on MoO3/SiO2 Catalysts: Application of Vibrational Spectroscopic Imaging Techniques in a High Throughput Operando Reactor." Topics in Catalysis 52. PNNL-SA-61599. 

Wang D., D. Choi, J. Li, Z. Yang, Z. Nie, R. Kou, and D. Hu, et al. 2009. "Self-assembled TiO2-Graphene Hybrid Nanostructures for Enhanced Li-ion Insertion." ACS Nano 3, no. 4:907-914. PNNL-SA-64760. 


Hu D., Z. Tian, Z. Tian, W. Wu, W. Wan, and A.D. Li. 2008. "Photoswitchable Nanoparticles Enable High-Resolution Cell Imaging: PULSAR Microscopy." Journal of the American Chemical Society 130, no. 46:15279-15281. PNNL-SA-60870. doi:10.1021/ja805948u 

Lei C., D. Hu, and E.J. Ackerman. 2008. "Single-molecule Fluorescence Spectroelectrochemistry of Cresyl Violet." Chemical Communications. PNNL-SA-61675. doi:10.1039/b812161c 

Li G., D. Hu, G. Xia, J.M. White, and Z.C. Zhang. 2008. "High Throughput Operando Studies using Fourier Transform Infrared Imaging and Raman Spectroscopy." Review of Scientific Instruments 79. PNNL-SA-60652. doi:10.1063/1.2949389 


Orr G., D.J. Panther, J.L. Phillips, B.J. Tarasevich, A. Dohnalkova, D. Hu, and J.G. Teeguarden, et al. 2007. "Submicron and Nanoscale Inorganic Particles Exploit the Actin Machinery to be Propelled Along Microvillilike Structures into Alveolar Cells." ACS Nano 1, no. 5:463-475. PNNL-SA-53625. doi:10.1021/nn700149r 

Pan D., D. Hu, R. Liu, X. Zeng, S. Kaplan, and H.P. Lu. 2007. "Fluctuating Two-State Light Harvesting in a Photosynthetic Membrane." Journal of Physical Chemistry C 111, no. 25:8948-8956. PNNL-SA-49243. doi:10.1021/jp071493y 


Hu D., and H.P. Lu. 2006. "Single Molecule Electron Transfer Process of Ruthenium Complexes." In Ultrasensitive and Single-Molecule Detection Technologies, 21-22 and 24 January 2006, San Jose, California, USA . Proceedings of the SPIE, edited by Jörg Enderlein and Zygmunt K. Gryczynski, 6092, 60927-1 -- 60927-7. Bellingham, Washington:Society of Photo-optical Instrumentation Engineers. PNNL-SA-48928. 

Liu R., D. Hu, X. Tan, and H.P. Lu. 2006. "Revealing Two-State Protein-Protein Interaction of Calmodulin by Single-Molecule Spectroscopy." Journal of the American Chemical Society 128, no. 31:10034-10042. PNNL-SA-46564. 

Pan D., N.A. Klymyshyn, D. Hu, and H.P. Lu. 2006. "Tip-Enhanced Near-Field Raman Spectroscopy Probing Single Dye-Sensitized TiO2 Nanoparticles." Applied Physics Letters 88, no. 9:Art. No. 093121. PNNL-SA-46223. 

Zhang T., S.N. Danthi, J. Xie, D. Hu, H.P. Lu, and K.H. Li. 2006. "Live Cell Imaging of the Endocytosis and the Intracellular Trafficking of Multifunctional Lipid Nanoparticles." In Proceedings of the SPIE, 6095, Art. No. 6095D. Bellingham, Washington:S P I E. PNNL-SA-52731. 


Hu D., and H.P. Lu. 2005. "Single-Molecule Triplet-State Photon Antibunching at Room Temperature." Journal of Physical Chemistry B 109, no. 20:9861-9864. PNNL-SA-43914. 

Link S., D. Hu, W.S. Chang, G.D. Scholes, and P.F. Barbara. 2005. "Nernatic Solvation of Segmented Polymer Chains." Nano Letters 5, no. 9:1757-1760. PNNL-SA-44706. doi:10.1021/nl051108l 

Orr G., D. Hu, S. Ozcelik, L. Opresko, H.S. Wiley, and S.D. Colson. 2005. "Cholesterol Dictates the Freedom of EGF Receptors and HER2 in the Plane of the Membrane." Biophysical Journal 89, no. 2:1362-1373. PNNL-SA-44634. doi:10.1529/biophysj.104.056192 

Pan D., D. Hu, and H.P. Lu. 2005. "Probing Inhomogeneous Vibrational Reorganization Energy Barriers of Interfacial Electron Transfer." Journal of Physical Chemistry B 109, no. 34:16390-16395. PNNL-SA-44187. 


Biju V.P., M. Micic, D. Hu, and H.P. Lu. 2004. "Intermittent Single-Molecule Interfacial Electron Transfer Dynamics." Journal of the American Chemical Society 126, no. 30:9374-9381. PNNL-SA-40763. 

Hu D., and H.P. Lu. 2004. "Placing Single-Molecule T4 Lysozyme Enzymes on a Bacterial Cell Surface: Toward Probing Single-Molecule Enzymatic Reaction in Living Cells." Biophysical Journal 87. PNNL-SA-41555. 

Hu D., M. Micic, N.A. Klymyshyn, Y.D. Suh, and H.P. Lu. 2004. "Correlated Topographic and Spectroscopic Imaging by Combined Atomic Force Microscopy and Optical Microscopy." Journal of Luminescence 107, no. 1-4:4-12. PNNL-SA-39849. 

Micic M., D. Hu, Y.D. Suh, G.J. Newton, M.F. Romine, and H.P. Lu. 2004. "Correlated Atomic Force Microscopy and Flourescence Lifetime Imaging of Live Bacterial Cells." Colloids and Surfaces. B, Biointerfaces 34. PNNL-SA-39208. 

Tan X., D. Hu, T.C. Squier, and H.P. Lu. 2004. "Probing Nanosecond Protein Motions of Calmodulin by Single-Molecule Fluorescence Anisotropy." Applied Physics Letters 85, no. 12:2420-2422. PNNL-SA-40127. 

Tan X., D. Hu, T.C. Squier, and H.P. Lu. 2004. "Probing Nanosecond Protein Motions of Calmodulin by Single-Molecule Fluorescence Anisotropy." Applied Physics Letters 85, no. 12:2420-2422. PNNL-SA-43285. 

Tan X., P. Nalbant, A. Toutchkine, D. Hu, E.R. Vorpagel, K.M. Hahn, and H.P. Lu. 2004. "Single-Molecule Study of Protein-Protein Interaction Dynamics in a Cell Signaling System." Journal of Physical Chemistry B 108, no. 2:737-744. PNNL-SA-38567. 


Chen Y., D. Hu, E.R. Vorpagel, and H.P. Lu. 2003. "Probing Single-Molecule T4 Lysozyme Conformational Dynamics by Intramolecular Fluorescence Energy Transfer." Journal of Physical Chemistry B 107. PNNL-SA-39706. 

Hu D., and H.P. Lu. 2003. "Single-Molecule Nanosecond Anisotropy Dynamics of Tethered Protein Motions." Journal of Physical Chemistry B 107, no. 2:618-626. PNNL-SA-36429. 

Hu D., M. Micic, N.A. Klymyshyn, Y.D. Suh, and H.P. Lu. 2003. "Correlated Topographic and Spectroscopic Imaging Beyond Diffraction Limit by Atomic Force Microscopy Metallic Tip-Enhanced Near-Field Flourescence Lifetime Microscopy." Review of Scientific Instruments 74, no. 7:3347-3355. PNNL-SA-37833. 


Hu, D., J. Yu, G. Padmanaban, S. Ramakrishnan, and P. F. Barbara. 2002. “Spatial Confinement of Exciton Transfer and the Role of Conformational Order in Organic Nanoparticles.” Nano Letters 2 (10): 1121-1124.  


Wong, K. F., M. S. Skaf, C. Y. Yang, P. J. Rossky, B. Bagchi, D. Hu, J. Yu, and P. F. Barbara. 2001. “Structural and Electronic Characterization of Chemical and Conformational Defects in Conjugated Polymers.” Journal of Physical Chemistry B 105 (26): 6103-6107.  

Yu, J., D. H. Hu, and P. F. Barbara. 2001. Photophysics of Conjugated Polymer Unmasked by Single Molecule Spectroscopy. In Springer Series in Chemical Physics. 


Hu, D., J. Yu, K. Wong, B. Bagchl, P. J. Rossky, and P. F. Barbara. 2000. “Collapse of Stiff Conjugated Polymers with Chemical Defects into Ordered, Cylindrical Conformations.” Nature 405 (6790): 1030-1033.  

Yu, J., D. Hu, and P. F. Barbara. 2000. “Unmasking Electronic Energy Transfer of Conjugated Polymers by Suppression of O2 Quenching.” Science 289 (5483): 1327-1330.


Hu, D., J. Yu, and P. F. Barbara. 1999. “Single-Molecule Spectroscopy of the Conjugated Polymer MEH-PPV.” Journal of the American Chemical Society 121 (29): 6936-6937.


Yip, W. T., D. Hu, J. Yu, D. A. Vanden Bout, and P. F. Barbara. 1998. “Classifying the Photophysical Dynamics of Single- and Multiple-Chromophoric Molecules by Single Molecule Spectroscopy.” Journal of Physical Chemistry A 102 (39): 7564-7575.


Olson, E. J. C., D. Hu, A. Hörmann, and P. F. Barbara. 1997. “Quantitative Modeling of DNA-Mediated Electron Transfer between Metallointercalators.” Journal of Physical Chemistry B 101 (3): 299-303.  

Olson, E. J. C., D. Hu, A. Hörmann, A. M. Jonkman, M. R. Arkin, E. D. A. Stemp, J. K. Barton, and P. F. Barbara. 1997. “First Observation of the Key Intermediate in the 'Light-Switch' Mechanism of [Ru(phen)2dppz]2+.” Journal of the American Chemical Society 119 (47): 11458-11467.  

Vanden Bout, D. A., W. T. Yip, D. Hu, D. K. Fu, T. M. Swager, and P. F. Barbara. 1997. “Discrete Intensity Jumps and Intramolecular Electronic Energy Transfer in the Spectroscopy of Single Conjugated Polymer Molecules.” Science 277 (5329): 1074-1077.