Cryo-EM Microscopist and Biochemist
Cryo-EM Microscopist and Biochemist

Biography

Amar Parvate is a biochemist at PNNL supporting the Environmental Molecular Sciences Laboratory (EMSL) user program. He specializes in cryo-electron microscopy (cryo-EM). His training includes biochemistry, cryo-EM, and tomography to perform structural analyses of viruses and antigen-antibody complexes. His current research involves using single-particle cryo-EM to solve structures of macromolecular assemblies of proteins of interest. 

 

Research Interests

  • Biochemistry 
  • Cryo-EM 
  • Protein engineering 
  • Protein structure 
  • Structural biology 
  • Virology 

Education

PhD in Biological Sciences, Purdue University, 2018

Awards and Recognitions

  • Bilsland Dissertation Fellowship, Purdue University (2018) 
  • Teaching Assistant Award, Department of Biological Sciences, Purdue University (2018) 

Publications

2026

  • Johnson C., Chan A., Rozum J., George A., Parvate A.D., Bohutskyi P., Kim D., Feng S., Johnson Z., Sadler N. C., Garcia M., Li X., Trejo J., Wu R., Sineath W., Anderson L., Evans J. E., Qian W., Mehta A., Luthey-Schulten Z., Cheung M. 4D Whole-cell Modeling of a Minimal Autotroph Reveals Carbon Metabolism Under Light Disturbance. Biophysical Journal (2026). https://doi.org/10.1016/j.bpj.2026.08.020
     
  • Donnellan, F.R., Rayaprolu, V., Rijal, P. O’Dowd V., Parvate A., Callaway H., Hariharan C., Parekh D., Hui S., Shaffer K.C.L., Diaz-Avlos R., Hastie K.M., Schimanski L., Müller-Kräuter H., Strecker T., Balaram A., Halfman P., Ollman Saphire E., Lightwood D.J.L, Townsend A.R., Draper S.J. A broadly-neutralizing antibody against Orthoebolavirus glycoprotein that potentiates the breadth and neutralization of other antibodies. npj Viruses (2026). https://doi.org/10.1038/s44298-026-00192-7
     
  • Parvate A. D., Alfaro T, McDearis R, Zimmerman A. E. , Hofmockel K. S., Nelson W. C., Evans J. E. Cryo-EM visualization of  viruses from partially irrigated soils. Front. Agron. 7:1711814. doi: 10.3389/fagro.2025.1711814
     
  • Bohyutski P, Parvate A. D., Sadler N. C., Chrisler W. B., Cheung M., Evans J. E. Systematic scale-up and enhanced purification of marine cyanophage P-SSP7. (2026) Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2026.1776133
     
  • Lee J. Y., Gandhi V. D., Harrilal C., Parvate A. D., Ives A., Bhotika H., Thibert S. M., Zhang X., Schenter G. K., Larriba-Andaluz C., Evans J. E., Ibrahim Y. M., and Garimella S. V. B. Characterization of Gas-Phase Native(-like) Proteins Using Structures for Lossless Ion Manipulations. Analytical Chemistry 2026 98 (1), 104-114. https://doi.org/10.1021/acs.analchem.5c02054

2025

  • Bunbury F., Rivas C., Calatrava V.,  Malkovskiy A.V.,  Joubert L.,  Parvate A.D. , Evans J.E. , Grossman A.R. ,  Bhaya D. Cyanobacteria and Chloroflexota cooperate to structure light-responsive biofilms, Proc. Natl. Acad. Sci. U.S.A. 122 (5) e2423574122,  https://doi.org/10.1073/pnas.2423574122 (2025).

2024

  • Jaichandran D., Parvate A.D., Brookreson J.T., Evans J.E, Chundawat S. P. S. Cell-free expression and biochemical characterization of polysaccharide-synthesizing glycosyltransferases (2024). Biochemical Engineering Journal. Vol 212, Article 109507. 10.1016/j.bej.2024.109507

2023

  • Rayaprolu V., Fulton B, Rafique A, Arturo E, Williams D, Hariharan C, Callaway H, Parvate A. D., Hui S, Shaffer K, Tramaglini K, Baum A, Kyratsous C, Saphire E. O. Structure of the Inmazeb cocktail and resistance against Ebola virus escape. 2023. Cell Host and Microbe (2023) https://www.cell.com/cell-host-microbe/issue?pii=S1931-3128(22)X0003-6.

2022

  • Parvate A. D., Powell S. M., Brookreson J. T., Moser T. H., Novikova I. V., Evans J. E. CryoEM structure of the diapause chaperone Artemin (2022). Frontiers Molecular Biosciences. https://doi.org/10.3389/fmolb.2022.998562.
     
  • Riahin C., Mendis K., Busick B., Ptaszek M., Yang M., Stacey G., Parvate A., Evans J. E., Traeger J., Hu D., Orr G., Rosenzweig Z. Near Infrared Emitting Semiconductor Polymer Dots for Bioimaging and Sensing (2022). MDPI Sensors https://doi.org/10.3390/s22197218
     
  • Peng W., Rayaprolu V., Parvate A.D., Pronker M.F., Hui S., Parekh D., Shaffer K., Yu X., Saphire E.O., Snijder J. Glycan shield of the ebola virus envelope glycoprotein GP (2022). Communications Biology. https://doi.org/10.1038/s42003-022-03767-1 
     

2020

  • Parvate A. D., Sengupta R, Williams E.P., Yue X, Chu YK, Stahlein R. V., Jonsson C. B., (2020). Cryofixation of inactivated hantavirus-infected cells as a method for obtaining high-quality ultrastructural preservation for electron microscopic studies. Front. Cell. Infect. Microbiol. https://doi.org/10.3389/fcimb.2020.580339 
     

2019

  • Parvate A. D., Hasan S. S., Vago F, Peters E. W., Lee J., Lanman J, Jonsson C, (2019). A New Inactivation Method to Facilitate CryoEM of Enveloped, RNA Viruses Requiring High Containment: A Case Study using Venezuelan Equine Encephalitis Virus (VEEV). Journal of Virological Methods. 277. 113792. 10.1016/j.jviromet.2019.113792
     
  • Parvate A. D., Williams E. P., Taylor M. K., Chu Y. K., Lanman J. K., Saphire E. Jonsson C. B. (2019) Diverse Morphology and Structural Features of Old and New World Hantaviruses. Viruses, 11 (862). https://doi.org/10.3390/v11090862