Agrobacterium-mediated transformation of lipomyces
This disclosure provides Agrobacterium-mediated transformation methods for the oil-producing (oleaginous) yeast Lipomyces sp., as well as yeast produced by the method. Such methods utilize Agrobacterium sp. cells that have a T-DNA binary plasmid, wherein the T-DNA binary plasmid comprises a first nucleic acid molecule encoding a first protein and a second nucleic acid molecule encoding a selective marker that permits growth of transformed Lipomyces sp. cells in selective culture media comprising an antibiotic.
ATLAS (Automatic Tool for Local Assembly Structures) - OPEN SOURCE
Whole community shotgun sequencing of total DNA (i.e. metagenomics) and total RNA (i.e. metatranscriptomics) has provided a wealth of information in the microbial community structure, predicted functions, metabolic networks, and is even able to reconstruct complete genomes directly. Here we present ATLAS (Automatic Tool for Local Assembly Structures) a comprehensive pipeline for assembly, annotation, genomic binning of metagenomic and metatranscriptomic data with an integrated framework for Multi-Omics. This will provide an open source tool for the Multi-Omic community at large.
SPARSE SAMPLING METHODS AND PROBE SYSTEMS FOR ANALYTICAL INSTRUMENTS
Sparse sampling approaches and probe systems for analytical instruments are disclosed providing for effective sub-sampling of a specimen and inpainting to reconstruct representations of actual information. The sub-sampling involves serial acquisition of contiguous measured values lying at positions along a scan path extending in a line toward a first direction and having random perturbations in a second direction. The perturbations are limited within a predetermined distance from the line. Inpainting techniques are utilized among the measured values to reconstruct a representation of actual information regarding the specimen.
Subwavelength coatings and methods for making and using same
Methods are disclosed for forming subwavelength coatings for use in the UV, visible, or infrared part of the electromagnetic spectrum. A first material and a second material are deposited onto a substrate. The first material may include dielectric spheres of subwavelength size that self-assemble on the substrate to form a template or scaffold with subwavelength size voids between the spheres into which the second material is deposited or filled. First and second materials are heated on the substrate at a preselected temperature to form the subwavelength coating.
Thermoelectric Devices and Applications for the Same
This invention describes a process for sputter deposition of thin films of alloys of Bi2Te3, Sb2Te3 and Bi2Se3 for thermoelectric energy conversion. The approach allows deposition of these films on glass and flexible substrates such as Kapton. The process was used to deposit n-type and p-type films that exhibit properties nearly as good as measured for bulk materials. A single thermocouple was formed from n-type and p-type films that produced 3 millivolts under a temperature difference of 10 degrees centigrade. This result demonstrates that miniature high-voltage, microwatt power sources can be constructed from thin film arrays deposited as described by the disclosed process.
Synthetic Transaction Test Generator for Websites
Python script for querying a list of web sites and their details from Splunk and dynamically creating tests for monitoring uptime. The data generated from this script is then sent back to Splunk for creating reports and alerts.
FREQUENCY THRESHOLD DETERMINATION FOR FREQUENCY-RESPONSIVE LOAD CONTROLLERS
The Grid FriendlyTM Appliance (GFA) controller, developed at Pacific Northwest National Laboratory, was originallydesigned to autonomously switch off appliances by detecting under-frequency events. In this paper, the feasibility of usingthe GFA controller to provide primary frequency response is investigated. In particular, the impacts of an important designparameter, i.e., curtailing frequency threshold, on the primary frequency response are carefully analyzed for different situations. In the normal situation, the current method of selecting curtailing frequency thresholds for GFAs is found to be insufficient to guarantee the desired performance especially when the frequency deviation is shallow. In the extreme situations, the power reduction of online GFAs could be so excessive that it can even impact the system frequency negatively. As the first step towards the efforts to make GFAs suitable for providing primary frequency response, the existing controller design is improved by modifying the strategy of selecting curtailing frequency thresholds to ensure the effectiveness of GFAs in the normal situation.
Isomorphic library for Ruby programming language
Isomorphic is a Ruby library for specifying isomorphisms between Ruby objects. The inventive features of the new Ruby library are as follows: Domain-specific language (DSL) for declaration of isomorphisms between Ruby objects. Functional references (viz., "lenses") for getter/setter operations.
Methods for Magnetic Resonance Analysis Using Magic Angle Technique
Previous work has shown that it is possible to separate the susceptibility broadening in the 1H NMR metabolite spectrum obtained in a live mouse from the isotropic information, thus significantly increasing the spectral resolution. This was achieved using ultra-slow magic angle spinning of the animal combined with a modified phase-corrected magic angle turning (PHORMAT) pulse sequence. However, PHORMAT cannot be used for spatially selective spectroscopy. In this invention a modified sequence called LOCMAT (localized magic angle turning) is introduced that makes this possible. Proton LOCMAT spectra are shown for the liver and heart of a live mouse, while spinning the animal at a speed of 4 Hz in a 2 Tesla field. It was found that even in this relatively low field LOCMAT provided isotropic line widths that are a factor 4-10 times smaller than the ones obtained in a stationary animal, and that the susceptibility broadening of the heart metabolites shows unusual features not observed for a dead animal. Finally, the limitations of LOCMAT and possible ways to improve the technique are discussed. It is concluded that in vivo LOCMAT can significantly enhance the utility of NMR spectroscopy for biomedical research.