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Fundamental and Computational Sciences Directorate

Staff information


Dehong Hu

Biosystms Dynamics & Simula
Pacific Northwest National Laboratory
PO Box 999
MSIN: K8-88
Richland, WA 99352

Research Interests

  • Single molecule spectroscopy
  • Microscopy and cell imaging
  • Ultrafast spectroscopy

Education and Credentials

  • Ph.D., Chemistry, University of Minnesota
  • M.S., Chemistry, Peking University
  • B.S., Chemistry, Peking University

Affiliations and Professional Service

  • Member of American Chemical Society
  • Senior editor of Nano Reviews

PNNL Publications


  • Cui Y., D. Hu, L. Markillie, W.B. Chrisler, M.J. Gaffrey, C.K. Ansong, and L. Sussel, et al. 2018. "Fluctuation localization imaging-based fluorescence in situ hybridization (fliFISH) for accurate detection and counting of RNA copies in single cells." Nucleic Acids Research 46, no. 2:Article No. e7. PNNL-SA-126413. doi:10.1093/nar/gkx874
  • Li X., J. Tao, D. Hu, M.H. Engelhard, W. Zhao, J. Zhang, and W. Xu. 2018. "Stability of Polymeric Separators in Lithium Metal Batteries in a Low Voltage Environment." Journal of Materials Chemistry A 6, no. 12:5006-5015. PNNL-SA-131076. doi:10.1039/C7TA11259A


  • Duan W., V.S. Vemuri, D. Hu, Z. Yang, and X. Wei. 2017. "A Protocol for Electrochemical Evaluation and State of Charge Diagnostics of A Symmetric Organic Redox Flow Battery." Journal of Visualized Experiments. PNNL-SA-118931. doi:10.3791/55171
  • Lemmer K.C., W. Zhang, S.J. Langer, A. Dohnalkova, D. Hu, R.A. Lemke, and J.S. Piotrowski, et al. 2017. "Mutations that alter the bacterial cell envelope increase lipid production." mBio 8, no. 3:Article No. e00513-17. PNNL-SA-125854. doi:10.1128/mBio.00513-17
  • Mobberley J.M., S.R. Lindemann, H.C. Bernstein, J.J. Moran, R.S. Renslow, J.T. Babauta, and D. Hu, et al. 2017. "Organismal and spatial partitioning of energy and macronutrient transformations within a hypersaline mat." FEMS Microbiology Ecology 93, no. 4:Article No. fix028. PNNL-SA-124562. doi:10.1093/femsec/fix028
  • Novikova I.V., C.R. Smallwood, Y. Gong, D. Hu, L. Hendricks, J.E. Evans, and A. Bhattarai, et al. 2017. "Multimodal Hyperspectral Optical Microscopy." Chemical Physics 498-499. PNNL-SA-120193. doi:10.1016/j.chemphys.2017.08.011
  • Varga T., T.C. Droubay, L. Kovarik, M.I. Nandasiri, V. Shutthanandan, D. Hu, and B. Kim, et al. 2017. "Coupled Lattice Polarization and Ferromagnetism in Multiferroic NiTiO3 Thin Films." ACS Applied Materials & Interfaces 9, no. 26:21879-21890. PNNL-SA-121771. doi:10.1021/acsami.7b04481
  • Wang Y., Z. Li, M. Liu, D. Hu, Y. Lin, and J. Li. 2017. "Multiple-Targeted Graphene-based Nanocarrier for Intracellular Imaging of mRNAs." Analytica Chimica Acta 983. PNNL-SA-126440. doi:10.1016/j.aca.2017.07.017


  • Duan W., V.S. Vemuri, J.D. Milshtein, S. Laramie, R.D. Dmello, J. Huang, and L. Zhang, et al. 2016. "A Symmetric Organic - Based Nonaqueous Redox Flow Battery and Its State of Charge Diagnostics by FTIR." Journal of Materials Chemistry A 4, no. 15:5448-5456. PNNL-SA-115896. doi:10.1039/c6ta01177b
  • El-Khoury P.Z., P. Abellan, R.L. Chantry, Y. Gong, A.G. Joly, I.V. Novikova, and J.E. Evans, et al. 2016. "The information content in single-molecule Raman nanoscopy." Advances in Physics: X 1, no. 1:35-54. PNNL-SA-114470. doi:10.1080/23746149.2016.1140010
  • Ford N.R., K.A. Hecht, D. Hu, G. Orr, Y. Xiong, T. Squier, and G.L. Rorrer, et al. 2016. "Antigen Binding and Site-Directed Labeling of Biosilica-Immobilized Fusion Proteins Expressed in Diatoms." ACS Synthetic Biology 5, no. 3:193-199. PNNL-SA-113297. doi:10.1021/acssynbio.5b00191
  • Melby E.S., A.C. Mensch, S.E. Lohse, D. Hu, G. Orr, C. Murphy, and R.J. Hamers, et al. 2016. "Formation of supported lipid bilayers containing phase-segregated domains and their interaction with gold nanoparticles." Environmental Science: Nano 3, no. 1:45-55. PNNL-SA-111557. doi:10.1039/C5EN00098J
  • Mitchell H.D., L. Markillie, W.B. Chrisler, M.J. Gaffrey, D. Hu, C.J. Szymanski, and Y. Xie, et al. 2016. "Cells Respond to Distinct Nanoparticle Properties with Multiple Strategies as Revealed by Single-Cell RNA-Seq." ACS Nano 10, no. 11:10173-10185. PNNL-SA-121323. doi:10.1021/acsnano.6b05452
  • Pomraning K.R., Y. Kim, C.D. Nicora, R.K. Chu, E.L. Bredeweg, S.O. Purvine, and D. Hu, et al. 2016. "Multi-omics analysis reveals regulators of the response to nitrogen limitation in Yarrowia lipolytica." BMC Genomics 17, no. 138:1-18. PNNL-SA-112784. doi:10.1186/s12864-016-2471-2
  • Proetto M.T., C.R. Anderton, D. Hu, C.J. Szymanski, Z. Zhu, J.P. Patterson, and J.K. Kammeyer, et al. 2016. "Cellular Delivery of Nanoparticles Revealed with Combined Optical and Isotopic Nanoscopy." ACS Nano 10, no. 4:4046-4054. PNNL-SA-106955. doi:10.1021/acsnano.5b06477
  • Schlecht W., K. Li, D. Hu, and W. Dong. 2016. "Fluorescence Based Characterization of Calcium Sensitizer Action on the Troponin Complex." Chemical Biology and Drug Design 87, no. 2:171-181. PNNL-SA-106744. doi:10.1111/cbdd.12651
  • Zhai Y., J.S. DuChene, Y. Wang, J. Qiu, A.C. Johnson-Peck, B. You, and W. Guo, et al. 2016. "Polyvinylpyrrolidone-induced anisotropic growth of gold nanoprisms in plasmon-driven synthesis." Nature Materials 15, no. 8:889-896. PNNL-SA-107281. doi:10.1038/nmat4683
  • Zhang J., G.N. Presley, K.E. Hammel, J.S. Ryu, J. Menke, M. Figueroa, and D. Hu, et al. 2016. "Localizing gene regulation reveals a staggered wood decay mechanism for the brown rot fungus Postia placenta." Proceedings of the National Academy of Sciences of the United States of America 113, no. 39:10968-10973. PNNL-SA-114266. doi:10.1073/pnas.1608454113
  • Zhang L., W. Jiang, A.C. Dissanayake, T. Varga, J. Zhang, Z. Zhu, and D. Hu, et al. 2016. "Grain growth of nanocrystalline 3C-SiC under Au ion irradiation at elevated temperatures." Journal of Physics D. Applied Physics 49, no. 3:035304. PNNL-SA-110626. doi:10.1088/0022-3727/49/3/035304


  • Chen W., E.W. Rosser, D. Zhang, W. Shi, Y. Li, W. Dong, and H. Ma, et al. 2015. "A Specific Nucleophilic Ring-Opening Reaction of Aziridines as a Unique Platform for the Construction of Hydrogen Polysulfides Sensors." Organic Letters 17, no. 11:2776-2779. PNNL-SA-108754. doi:10.1021/acs.orglett.5b01194
  • El-Khoury P.Z., Y. Gong, P. Abellan, B.W. Arey, A.G. Joly, D. Hu, and J.E. Evans, et al. 2015. "Tip-Enhanced Raman Nanographs: Mapping Topography and Local Electric Fields." Nano Letters 15, no. 4:2385-2390. PNNL-SA-106991. doi:10.1021/acs.nanolett.5b00609
  • Gong Y., A.G. Joly, D. Hu, P.Z. El-Khoury, and W.P. Hess. 2015. "Ultrafast Imaging of Surface Plasmons Propagating on a Gold Surface." Nano Letters 15, no. 5:3472-3478. PNNL-SA-108438. doi:10.1021/acs.nanolett.5b00803
  • Grate J.W., K. Mo, Y. Shin, A. Vasdekis, M.G. Warner, R.T. Kelly, and G. Orr, et al. 2015. "Alexa Fluor-labeled Fluorescent Cellulose Nanocrystals for Bioimaging Solid Cellulose in Spatially Structured Microenvironments." Bioconjugate Chemistry 26, no. 3:593-601. PNNL-SA-88815. doi:10.1021/acs.bioconjchem.5b00048
  • Jacobson K.H., I.L. Gunsolus, T.R. Kuech, J.M. Troiano, E.S. Melby, S.E. Lohse, and D. Hu, et al. 2015. "Lipopolysaccharide density and structure govern the extent and distance of nanoparticle interaction with actual and model bacterial outer membranes." Environmental Science & Technology 49, no. 17:10642-10652. PNNL-SA-112154. doi:10.1021/acs.est.5b01841
  • Liu T.L., J.T. Cox, D. Hu, X. Deng, J.Z. Hu, M.Y. Hu, and J. Xiao, et al. 2015. "A Fundamental Study on the [(µ-Cl)3Mg2(THF)6]+ Dimer Electrolytes for Rechargeable Mg Batteries." Chemical Communications 51, no. 12:2312-2315. PNNL-SA-104532. doi:10.1039/c4cc07621d
  • Lu N., A. Massoudieh, X. Liang, D. Hu, T. Kamai, T.R. Ginn, and J.L. Zilles, et al. 2015. "Swimming motility reduces Azotobacter vinelandii deposition to silica surfaces." Journal of Environmental Quality 44, no. 5:1366-1375. PNNL-SA-103558. doi:10.2134/jeq2015.03.0141
  • Mihai C., W.B. Chrisler, Y. Xie, D. Hu, C.J. Szymanski, A. Tolic, and J. Klein, et al. 2015. "Intracellular accumulation dynamics and fate of zinc ions in alveolar epithelial cells exposed to airborne ZnO nanoparticles at the air-liquid interface." Nanotoxicology 9, no. 1:9-22. PNNL-SA-99125. doi:10.3109/17435390.2013.859319
  • Szymanski C.J., P. Munusamy, C. Mihai, Y. Xie, D. Hu, M.K. Gilles, and T. Tyliszczak, et al. 2015. "Shifts in oxidation states of cerium oxide nanoparticles detected inside intact hydrated cells and organelles." Biomaterials 62. PNNL-SA-110620. doi:10.1016/j.biomaterials.2015.05.042
  • Troiano J., L.L. Olenick, T.R. Kuech, E.S. Melby, D. Hu, S.E. Lohse, and A.C. Mensch, et al. 2015. "Direct Probes of 4 nm Diameter Gold Nanoparticles Interacting with Supported Lipid Bylayers." Journal of Physical Chemistry C 119, no. 1:534-546. PNNL-SA-102945. doi:10.1021/jp512107z
  • Varga T., T.C. Droubay, M.E. Bowden, L. Kovarik, D. Hu, and S.A. Chambers. 2015. "Strain-dependence Of The Structure And Ferroic Properties Of Epitaxial NiTiO3 Thin Films Grown On Different Substrates." Advances in Condensed Matter Physics 2015. PNNL-SA-110090. doi:10.1155/2015/493045
  • Varga T., T.C. Droubay, M.E. Bowden, S.A. Stephens, S. Manandhar, V. Shutthanandan, and R.J. Colby, et al. 2015. "Strain-dependence Of The Structure And Ferroic Properties Of Epitaxial Ni-1 (-) Ti-x(1) (-) O-y(3) Thin Films Grown On Sapphire Substrates." Thin Solid Films 578. PNNL-SA-106024. doi:10.1016/j.tsf.2015.02.016
  • Wang Z., B. Liu, E. Zhao, K. Jin, Y. Du, J.J. Neeway, and J.V. Ryan, et al. 2015. "Argon Cluster Sputtering Source for ToF-SIMS Depth Profiling of Insulating Materials: High Sputter Rate and Accurate Interfacial Information." Journal of the American Society for Mass Spectrometry 26, no. 8:1283-1290. PNNL-SA-105691. doi:10.1007/s13361-015-1159-1


  • Cole J.K., J.R. Hutchison, R.S. Renslow, Y. Kim, W.B. Chrisler, H.E. Engelmann, and A. Dohnalkova, et al. 2014. "Phototrophic Biofilm Assembly in Microbial-Mat-Derived Unicyanobacterial Consortia: Model Systems for the Study of Autotroph-Heterotroph Interactions." Frontiers in Microbiology 5. PNNL-SA-99657. doi:10.3389/fmicb.2014.00109
  • El-Khoury P.Z., E. Khon, Y. Gong, A.G. Joly, P. Abellan, J.E. Evans, and N.D. Browning, et al. 2014. "Electric Field Enhancement in a Self-Assembled 2D Array of Silver Nanospheres." Journal of Chemical Physics 141, no. 21:Article No. 214308. PNNL-SA-105010. doi:10.1063/1.4902905
  • El-Khoury P.Z., T.W. Ueltschi, A.L. Mifflin, D. Hu, and W.P. Hess. 2014. "Frequency Resolved Nanoscale Chemical Imaging of 4,4'-Dimercaptostilbene on Silver." Journal of Physical Chemistry C 118, no. 47:27525-27530. PNNL-SA-100249. doi:10.1021/jp509082c
  • Gunsolus I.L., D. Hu, C. Mihai, S.E. Lohse, C. Lee, M. Torelli, and R.J. Hamers, et al. 2014. "Facile method to stain the bacterial cell surface for super-resolution fluorescence microscopy." Analyst 139, no. 12:3174-3178. PNNL-SA-102724. doi:10.1039/c4an00574k


  • Brown E., D. Hu, N. Abu-Lail, and X. Zhang. 2013. "Potential of nanocrystalline cellulose-fibrin nanocomposites for artificial vascular graft applications." Biomacromolecules 14, no. 4:1063-1071. PNNL-SA-89375.
  • Chien M., A.S. Carlini, D. Hu, C.V. Barback, A.M. Rush, D.J. Hall, and G. Orr, et al. 2013. "Enzyme-Directed Assembly of Nanoparticles in Tumors Monitored by In Vivo Whole Animal and Ex Vivo Super-Resolution Fluorescence Imaging." Journal of the American Chemical Society 135, no. 50:18710-18713. PNNL-SA-98263. doi:10.1021/ja408182p
  • El-Khoury P.Z., D. Hu, and W.P. Hess. 2013. "Junction Plasmon-Induced Molecular Reorientation." Journal of Physical Chemistry Letters 4, no. 20:3435-3439. PNNL-SA-97929. doi:10.1021/jz401838b
  • El-Khoury P.Z., D. Hu, V. Apkarian, and W.P. Hess. 2013. "Raman Scattering at Plasmonic Junctions Shorted by Conductive Molecular Bridges." Nano Letters 13, no. 4:1858-1861. PNNL-SA-93981. doi:10.1021/nl400733r
  • El-Khoury P.Z., S.J. Peppernick, D. Hu, A.G. Joly, and W.P. Hess. 2013. "The Origin of Surface-Enhanced Raman Scattering of 4,4' -Biphenyldicarboxylate on Silver Substrates." Journal of Physical Chemistry C 117, no. 14:7260-7268. PNNL-SA-92092. doi:10.1021/jp401026x
  • Geng H., S. Pan, and D. Hu. 2013. "Electrogenerated Chemiluminescence and Fluorescence Lifetime Spatial Heterogeneity of Poly (2-methoxy-5-(2-ethylhexyloxy)-1, 4-phenylenevinylene) in Presence of [6,6]-phenyl-C61-butyric Acid Methyl Ester." In MRS Proceedings: Symposium E/H - Photovoltaic Technologies, Devices and Systems Based on Inorganic Materials, Small Organic Molecules and Hybrids, 1493, Paper No. mrsf12-1493-h01-09. Warrendale, Pennsylvania:Materials Research Society. PNNL-SA-91989. doi:10.1557/opl.2013.19
  • Hu D., B. Zhao, Y. Xie, G. Orr, and A.D. Li. 2013. "Understanding Super-Resolution Nanoscopy and Its Biological Applications in Cell Imaging." Physical Chemistry Chemical Physics. PCCP 15, no. 36:14856-14861. PNNL-SA-95464. doi:10.1039/C3CP51629F
  • Liu Y., C. Zhang, D. Hu, M.S. Kuhlenschmidt, T.B. Kuhlenschmidt, S.E. Mylon, and R. Kong, et al. 2013. "Role of collector alternating charged patches on transport of Cryptosporidium parvum oocyst in a patchwise charged heterogeneous micromodel." Environmental Science & Technology 47, no. 6:2670-2678. PNNL-SA-91345. doi:10.1021/es304075j
  • Nasybulin E.N., W. Xu, M.H. Engelhard, X.S. Li, M. Gu, D. Hu, and J. Zhang. 2013. "Electrocatalytic properties of poly(3,4-ethylenedioxythiophene) (PEDOT) in Li-O2 battery." Electrochemistry Communications 29. PNNL-SA-92140. doi:10.1016/j.elecom.2013.01.011
  • Varga T., T.C. Droubay, M.E. Bowden, R.J. Colby, S. Manandhar, V. Shutthanandan, and D. Hu, et al. 2013. "Coexistence of Weak Ferromagnetism and Polar Lattice Distortion in Epitaxial NiTiO3 thin films of the LiNbO3-Type Structure." Journal of Vacuum Science and Technology B--Microelectronics and Nanometer Structures 31, no. 3:Article No. 030603. PNNL-SA-92833. doi:10.1116/1.4801664
  • Wang Y., Z. Li, T.J. Weber, D. Hu, C.T. Lin, J. Li, and Y. Lin. 2013. "In Situ Live Cell Sensing of Multiple Nucleotides Exploiting DNA/RNA Aptamers and Graphene Oxide Nanosheets." Analytical Chemistry 85, no. 14:6775-6782. PNNL-SA-96634. doi:10.1021/ac400858g


  • Chen X., W. Xu, J. Xiao, M.H. Engelhard, F. Ding, D. Mei, and D. Hu, et al. 2012. "Effects of cell positive cans and separators on the performance of high-voltage Li-ion batteries." Journal of Power Sources 213. PNNL-SA-84951. doi:10.1016/j.jpowsour.2012.04.009
  • Clayton D.A., T. McPherson, S. Pan, M. Chen, D.A. Dixon, and D. Hu. 2012. "Spatial and temporal variation of surface-enhanced Raman scattering at Ag nanowires in aqueous solution." Physical Chemistry Chemical Physics. PCCP 15, no. 3:850-859. PNNL-SA-82982.
  • Jin H., Y. Yu, W.B. Chrisler, Y. Xiong, D. Hu, and C. Lei. 2012. "Delivery of MicroR A-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing." Breast Cancer: Basic and Clinical Research 6. PNWD-SA-9642. doi:10.4137/BCBCR.S8513
  • Xu W., A.L. Read, P.K. Koech, D. Hu, C.M. Wang, J. Xiao, and A.B. Padmaperuma, et al. 2012. "Factors Affecting the Battery Performance of Anthraquinone-based Organic Cathode Materials." Journal of Materials Chemistry 22, no. 9:4032-4039. PNNL-SA-83987. doi:10.1039/c2jm15764k


  • Li A.D., C. Zhan, D. Hu, W. Wan, and J. Yao. 2011. "Photoswitchable Nanoprobes Offer Unlimited Brightness in Frequency-Domain Imaging." Journal of the American Chemical Society 133, no. 20:7628-7631. PNNL-SA-75462. doi:10.1021/ja1108479
  • Tian Z., Z. Tian, A.D. Li, and D. Hu. 2011. "Super-Resolution Fluorescence Nanoscopy Applied to Image Core-Shell Photoswitching Nanoparticles and their Self-Assemblies." Chemical Communications 47, no. 4:1258-1260. PNNL-SA-71515. doi:10.1039/C0CC03217D
  • Xiao J., J.Z. Hu, D. Wang, D. Hu, W. Xu, G.L. Graff, and Z. Nie, et al. 2011. "Investigation of the Rechargeability of Li-O2 Batteries in Non-aqueous Electrolyte." Journal of Power Sources 196, no. 13:5674-5678. PNNL-SA-75791.
  • Xu W., K. Xu, V.V. Viswanathan, S.A. Towne, J.S. Hardy, J. Xiao, and Z. Nie, et al. 2011. "Reaction Mechanisms for the Limited Reversibility of Li-O2 Chemistry in Organic Carbonate Electrolytes." Journal of Power Sources 196, no. 22:9631-9639. PNNL-SA-78429. doi:10.1016/j.jpowsour.2011.06.099
  • Xu W., V.V. Viswanathan, D. Wang, S.A. Towne, J. Xiao, Z. Nie, and D. Hu, et al. 2011. "Investigation on the Charging Process of Li2O2-Based Air Electrodes in Li-O2 Batteries with Organic Carbonate Electrolytes." Journal of Power Sources 196, no. 8:3894-3899. PNNL-SA-75700.


  • Hu D., and G. Orr. 2010. "Nanometer Resolution Imaging by SIngle Molecule Switching." Nano Reviews 1. PNNL-SA-70811. doi:10.3402/nano.v1i0.5122
  • Li G., D. Hu, G. Xia, and Z.C. Zhang. 2010. "Catalyst Structure-Performance Relationship Identified by High-Throughput Operando Method: New Insight for Silica-Supported Vanadium Oxide for Methanol Oxidation." Topics in Catalysis 53, no. 1-2:40-48. PNNL-SA-58799.
  • Wang D., R. Kou, D. Choi, Z. Yang, Z. Nie, J. Li, and L.V. Saraf, et al. 2010. "Ternary Self-Assembly of Ordered Metal Oxide-Graphene Nanocomposites for Electrochemical Energy Storage." ACS Nano 4, no. 3:1587-1595. PNNL-SA-70609. doi:10.1021/nn901819n
  • Wang Y., Z. Li, D. Hu, C.T. Lin, J. Li, and Y. Lin. 2010. "Aptamer/Graphene Oxide Nanocomplex for In Situ Molecular Probing in Living Cells." Journal of the American Chemical Society 132, no. 27:9274-9276. PNNL-SA-73086. doi:10.1021/ja103169v


  • Hu D., C. Lei, and E.J. Ackerman. 2009. "Single-molecule electron transfer reactions in nanomaterials." In Microscopy and Microanalysis 2009: Proceedings, July 26-30, 2009, Richmond, Virginia, edited by LN Brewer, 15, 1138-1139. New York:Cambridge University Press. PNNL-SA-64503. doi:10.1017/S1431927609095178
  • Hu D., Z. Tian, Z. Tian, W. Wu, W. Wan, and A.D. Li. 2009. "Single-Molecule Photoswitching Enables High-Resolution Optical Imaging." In Microscopy and Microanalysis, 15, 840-841. Cambridge:Cambridge University Press. PNNL-SA-64857.
  • Lei C., D. Hu, and E.J. Ackerman. 2009. "Clay Nanoparticle-Supported Single-Molecule Fluorescence Spectroelectrochemistry." Nano Letters 9, no. 2:655-658. PNNL-SA-61456.
  • Li G., D. Hu, G. Xia, and Z.C. Zhang. 2009. "Methanol Partial Oxidation on MoO3/SiO2 Catalysts: Application of Vibrational Spectroscopic Imaging Techniques in a High Throughput Operando Reactor." Topics in Catalysis 52. PNNL-SA-61599.
  • Wang D., D. Choi, J. Li, Z. Yang, Z. Nie, R. Kou, and D. Hu, et al. 2009. "Self-assembled TiO2-Graphene Hybrid Nanostructures for Enhanced Li-ion Insertion." ACS Nano 3, no. 4:907-914. PNNL-SA-64760.


  • Hu D., Z. Tian, Z. Tian, W. Wu, W. Wan, and A.D. Li. 2008. "Photoswitchable Nanoparticles Enable High-Resolution Cell Imaging: PULSAR Microscopy." Journal of the American Chemical Society 130, no. 46:15279-15281. PNNL-SA-60870. doi:10.1021/ja805948u
  • Lei C., D. Hu, and E.J. Ackerman. 2008. "Single-molecule Fluorescence Spectroelectrochemistry of Cresyl Violet." Chemical Communications. PNNL-SA-61675. doi:10.1039/b812161c
  • Li G., D. Hu, G. Xia, J.M. White, and Z.C. Zhang. 2008. "High Throughput Operando Studies using Fourier Transform Infrared Imaging and Raman Spectroscopy." Review of Scientific Instruments 79. PNNL-SA-60652. doi:10.1063/1.2949389


  • Orr G., D.J. Panther, J.L. Phillips, B.J. Tarasevich, A. Dohnalkova, D. Hu, and J.G. Teeguarden, et al. 2007. "Submicron and Nanoscale Inorganic Particles Exploit the Actin Machinery to be Propelled Along Microvillilike Structures into Alveolar Cells." ACS Nano 1, no. 5:463-475. PNNL-SA-53625. doi:10.1021/nn700149r
  • Pan D., D. Hu, R. Liu, X. Zeng, S. Kaplan, and H.P. Lu. 2007. "Fluctuating Two-State Light Harvesting in a Photosynthetic Membrane." Journal of Physical Chemistry C 111, no. 25:8948-8956. PNNL-SA-49243. doi:10.1021/jp071493y


  • Hu D., and H.P. Lu. 2006. "Single Molecule Electron Transfer Process of Ruthenium Complexes." In Ultrasensitive and Single-Molecule Detection Technologies, 21-22 and 24 January 2006, San Jose, California, USA . Proceedings of the SPIE, edited by Jörg Enderlein and Zygmunt K. Gryczynski, 6092, 60927-1 -- 60927-7. Bellingham, Washington:Society of Photo-optical Instrumentation Engineers. PNNL-SA-48928.
  • Liu R., D. Hu, X. Tan, and H.P. Lu. 2006. "Revealing Two-State Protein-Protein Interaction of Calmodulin by Single-Molecule Spectroscopy." Journal of the American Chemical Society 128, no. 31:10034-10042. PNNL-SA-46564.
  • Pan D., N.A. Klymyshyn, D. Hu, and H.P. Lu. 2006. "Tip-Enhanced Near-Field Raman Spectroscopy Probing Single Dye-Sensitized TiO2 Nanoparticles." Applied Physics Letters 88, no. 9:Art. No. 093121. PNNL-SA-46223.
  • Zhang T., S.N. Danthi, J. Xie, D. Hu, H.P. Lu, and K.H. Li. 2006. "Live Cell Imaging of the Endocytosis and the Intracellular Trafficking of Multifunctional Lipid Nanoparticles." In Proceedings of the SPIE, 6095, Art. No. 6095D. Bellingham, Washington:S P I E. PNNL-SA-52731.


  • Hu D., and H.P. Lu. 2005. "Single-Molecule Triplet-State Photon Antibunching at Room Temperature." Journal of Physical Chemistry B 109, no. 20:9861-9864. PNNL-SA-43914.
  • Link S., D. Hu, W.S. Chang, G.D. Scholes, and P.F. Barbara. 2005. "Nernatic Solvation of Segmented Polymer Chains." Nano Letters 5, no. 9:1757-1760. PNNL-SA-44706. doi:10.1021/nl051108l
  • Orr G., D. Hu, S. Ozcelik, L. Opresko, H.S. Wiley, and S.D. Colson. 2005. "Cholesterol Dictates the Freedom of EGF Receptors and HER2 in the Plane of the Membrane." Biophysical Journal 89, no. 2:1362-1373. PNNL-SA-44634. doi:10.1529/biophysj.104.056192
  • Pan D., D. Hu, and H.P. Lu. 2005. "Probing Inhomogeneous Vibrational Reorganization Energy Barriers of Interfacial Electron Transfer." Journal of Physical Chemistry B 109, no. 34:16390-16395. PNNL-SA-44187.


  • Biju V.P., M. Micic, D. Hu, and H.P. Lu. 2004. "Intermittent Single-Molecule Interfacial Electron Transfer Dynamics." Journal of the American Chemical Society 126, no. 30:9374-9381. PNNL-SA-40763.
  • Hu D., and H.P. Lu. 2004. "Placing Single-Molecule T4 Lysozyme Enzymes on a Bacterial Cell Surface: Toward Probing Single-Molecule Enzymatic Reaction in Living Cells." Biophysical Journal 87. PNNL-SA-41555.
  • Hu D., M. Micic, N.A. Klymyshyn, Y.D. Suh, and H.P. Lu. 2004. "Correlated Topographic and Spectroscopic Imaging by Combined Atomic Force Microscopy and Optical Microscopy." Journal of Luminescence 107, no. 1-4:4-12. PNNL-SA-39849.
  • Micic M., D. Hu, Y.D. Suh, G.J. Newton, M.F. Romine, and H.P. Lu. 2004. "Correlated Atomic Force Microscopy and Flourescence Lifetime Imaging of Live Bacterial Cells." Colloids and Surfaces. B, Biointerfaces 34. PNNL-SA-39208.
  • Tan X., D. Hu, T.C. Squier, and H.P. Lu. 2004. "Probing Nanosecond Protein Motions of Calmodulin by Single-Molecule Fluorescence Anisotropy." Applied Physics Letters 85, no. 12:2420-2422. PNNL-SA-40127.
  • Tan X., D. Hu, T.C. Squier, and H.P. Lu. 2004. "Probing Nanosecond Protein Motions of Calmodulin by Single-Molecule Fluorescence Anisotropy." Applied Physics Letters 85, no. 12:2420-2422. PNNL-SA-43285.
  • Tan X., P. Nalbant, A. Toutchkine, D. Hu, E.R. Vorpagel, K.M. Hahn, and H.P. Lu. 2004. "Single-Molecule Study of Protein-Protein Interaction Dynamics in a Cell Signaling System." Journal of Physical Chemistry B 108, no. 2:737-744. PNNL-SA-38567.


  • Chen Y., D. Hu, E.R. Vorpagel, and H.P. Lu. 2003. "Probing Single-Molecule T4 Lysozyme Conformational Dynamics by Intramolecular Fluorescence Energy Transfer." Journal of Physical Chemistry B 107. PNNL-SA-39706.
  • Hu D., and H.P. Lu. 2003. "Single-Molecule Nanosecond Anisotropy Dynamics of Tethered Protein Motions." Journal of Physical Chemistry B 107, no. 2:618-626. PNNL-SA-36429.
  • Hu D., M. Micic, N.A. Klymyshyn, Y.D. Suh, and H.P. Lu. 2003. "Correlated Topographic and Spectroscopic Imaging Beyond Diffraction Limit by Atomic Force Microscopy Metallic Tip-Enhanced Near-Field Flourescence Lifetime Microscopy." Review of Scientific Instruments 74, no. 7:3347-3355. PNNL-SA-37833.

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