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Fundamental and Computational Sciences Directorate

Staff information


Dehong Hu

Biosystms Dynamics & Simula
Pacific Northwest National Laboratory
PO Box 999
MSIN: K8-88
Richland, WA 99352

Research Interests

  • Single molecule spectroscopy
  • Microscopy and cell imaging
  • Ultrafast spectroscopy

Education and Credentials

  • Ph.D., Chemistry, University of Minnesota
  • M.S., Chemistry, Peking University
  • B.S., Chemistry, Peking University

Affiliations and Professional Service

  • Member of American Chemical Society
  • Senior editor of Nano Reviews

PNNL Publications


  • Cui Y, D Hu, LM Markillie, WB Chrisler, MJ Gaffrey, CK Ansong, L Sussel, and G Orr. 2018. "Fluctuation localization imaging-based fluorescence in situ hybridization (fliFISH) for accurate detection and counting of RNA copies in single cells." Nucleic Acids Research 46(2):Article No. e7.  doi:10.1093/nar/gkx874
  • Li X, J Tao, D Hu, MH Engelhard, W Zhao, J Zhang, and W Xu. 2018. "Stability of Polymeric Separators in Lithium Metal Batteries in a Low Voltage Environment." Journal of Materials Chemistry A 6(12):5006-5015.  doi:10.1039/C7TA11259A


  • Novikova IV, CR Smallwood, Y Gong, D Hu, L Hendricks, JE Evans, A Bhattarai, WP Hess, and PZ El-Khoury. 2017. "Multimodal Hyperspectral Optical Microscopy." Chemical Physics 498-499:25-32.  doi:10.1016/j.chemphys.2017.08.011
  • Varga T, TC Droubay, L Kovarik, MI Nandasiri, V Shutthanandan, D Hu, B Kim, S Jeon, S Hong, Y Li, and SA Chambers. 2017. "Coupled Lattice Polarization and Ferromagnetism in Multiferroic NiTiO3 Thin Films." ACS Applied Materials & Interfaces 9(26):21879-21890.  doi:10.1021/acsami.7b04481
  • Duan W, VRS Vemuri, D Hu, Z Yang, and X Wei. 2017. "A Protocol for Electrochemical Evaluation and State of Charge Diagnostics of A Symmetric Organic Redox Flow Battery." Journal of Visualized Experiments (120):Video No. e55171.  doi:10.3791/55171
  • Lemmer KC, W Zhang, SJ Langer, A Dohnalkova, D Hu, RA Lemke, JS Piotrowski, G Orr, DR Noguera, and TJ Donohue. 2017. "Mutations that alter the bacterial cell envelope increase lipid production." mBio 8(3):Article No. e00513-17.  doi:10.1128/mBio.00513-17
  • Mobberley JM, SR Lindemann, HC Bernstein, JJ Moran, RS Renslow, JT Babauta, D Hu, H Beyenal, and WC Nelson. 2017. "Organismal and spatial partitioning of energy and macronutrient transformations within a hypersaline mat." FEMS Microbiology Ecology 93(4):Article No. fix028.  doi:10.1093/femsec/fix028
  • Wang Y, Z Li, M Liu, D Hu, Y Lin, and J Li. 2017. "Multiple-Targeted Graphene-based Nanocarrier for Intracellular Imaging of mRNAs." Analytica Chimica Acta 983:1-8.  doi:10.1016/j.aca.2017.07.017


  • Melby ES, AC Mensch, SE Lohse, D Hu, G Orr, C Murphy, RJ Hamers, and JA Pedersen. 2016. "Formation of supported lipid bilayers containing phase-segregated domains and their interaction with gold nanoparticles." Environmental Science: Nano 3(1):45-55.  doi:10.1039/C5EN00098J
  • Duan W, VRS Vemuri, JD Milshtein, S Laramie, RD Dmello, J Huang, L Zhang, D Hu, M Vijayakumar, W Wang, J Liu, RE Darling, L Thompson, KC Smith, JS Moore, F Brushett, and X Wei. 2016. "A Symmetric Organic - Based Nonaqueous Redox Flow Battery and Its State of Charge Diagnostics by FTIR." Journal of Materials Chemistry A 4(15):5448-5456.  doi:10.1039/c6ta01177b
  • El-Khoury PZ, P Abellan, RL Chantry, Y Gong, AG Joly, IV Novikova, JE Evans, E Apra, D Hu, QM Ramasse, and WP Hess. 2016. "The information content in single-molecule Raman nanoscopy." Advances in Physics: X 1(1):35-54.  doi:10.1080/23746149.2016.1140010
  • Ford NR, KA Hecht, D Hu, G Orr, Y Xiong, T Squier, GL Rorrer, and G Roesijadi. 2016. "Antigen Binding and Site-Directed Labeling of Biosilica-Immobilized Fusion Proteins Expressed in Diatoms." ACS Synthetic Biology 5(3):193-199.  doi:10.1021/acssynbio.5b00191
  • Mitchell HD, LM Markillie, WB Chrisler, MJ Gaffrey, D Hu, CJ Szymanski, Y Xie, ES Melby, A Dohnalkova, RC Taylor, EK Grate, SK Cooley, JE McDermott, A Heredia-Langner, and G Orr. 2016. "Cells Respond to Distinct Nanoparticle Properties with Multiple Strategies as Revealed by Single-Cell RNA-Seq." ACS Nano 10(11):10173-10185.  doi:10.1021/acsnano.6b05452
  • Pomraning KR, YM Kim, CD Nicora, RK Chu, EL Bredeweg, SO Purvine, D Hu, TO Metz, and SE Baker. 2016. "Multi-omics analysis reveals regulators of the response to nitrogen limitation in Yarrowia lipolytica." BMC Genomics 17(138):1-18.  doi:10.1186/s12864-016-2471-2
  • Proetto MT, CR Anderton, D Hu, CJ Szymanski, Z Zhu, JP Patterson, JK Kammeyer, LG Nilewski, AM Rush, NC Bell, JE Evans, G Orr, SB Howell, and NC Gianneschi. 2016. "Cellular Delivery of Nanoparticles Revealed with Combined Optical and Isotopic Nanoscopy." ACS Nano 10(4):4046-4054.  doi:10.1021/acsnano.5b06477
  • Schlecht W, KL Li, D Hu, and WJ Dong. 2016. "Fluorescence Based Characterization of Calcium Sensitizer Action on the Troponin Complex." Chemical Biology and Drug Design 87(2):171-181.  doi:10.1111/cbdd.12651
  • Zhai Y, JS DuChene, YC Wang, J Qiu, AC Johnson-Peck, B You, W Guo, B DiCiaccio, K Qian, E Zhao, F Ooi, D Hu, D Su, EA Stach, Z Zhu, and WD Wei. 2016. "Polyvinylpyrrolidone-induced anisotropic growth of gold nanoprisms in plasmon-driven synthesis." Nature Materials 15(8):889-896.  doi:10.1038/nmat4683
  • Zhang J, GN Presley, KE Hammel, JS Ryu, J Menke, M Figueroa, D Hu, G Orr, and JS Schilling. 2016. "Localizing gene regulation reveals a staggered wood decay mechanism for the brown rot fungus Postia placenta." Proceedings of the National Academy of Sciences of the United States of America 113(39):10968-10973.  doi:10.1073/pnas.1608454113
  • Zhang L, W Jiang, AC Dissanayake, T Varga, J Zhang, Z Zhu, D Hu, H Wang, CH Henager, Jr, and T Wang. 2016. "Grain growth of nanocrystalline 3C-SiC under Au ion irradiation at elevated temperatures." Journal of Physics D. Applied Physics 49(3):035304.  doi:10.1088/0022-3727/49/3/035304


  • Gong Y, AG Joly, D Hu, PZ El-Khoury, and WP Hess. 2015. "Ultrafast Imaging of Surface Plasmons Propagating on a Gold Surface." Nano Letters 15(5):3472-3478.  doi:10.1021/acs.nanolett.5b00803
  • Varga T, TC Droubay, ME Bowden, L Kovarik, D Hu, and SA Chambers. 2015. "Strain-dependence Of The Structure And Ferroic Properties Of Epitaxial NiTiO3 Thin Films Grown On Different Substrates." Advances in Condensed Matter Physics 2015:Art. No. 493045.  doi:10.1155/2015/493045
  • Varga T, TC Droubay, ME Bowden, SA Stephens, S Manandhar, V Shutthanandan, RJ Colby, D Hu, WA Shelton, and SA Chambers. 2015. "Strain-dependence Of The Structure And Ferroic Properties Of Epitaxial Ni-1 (-) Ti-x(1) (-) O-y(3) Thin Films Grown On Sapphire Substrates." Thin Solid Films 578:113-123.  doi:10.1016/j.tsf.2015.02.016
  • Chen W, EW Rosser, D Zhang, W Shi, Y Li, WJ Dong, H Ma, D Hu, and M Xian. 2015. "A Specific Nucleophilic Ring-Opening Reaction of Aziridines as a Unique Platform for the Construction of Hydrogen Polysulfides Sensors." Organic Letters 17(11):2776-2779.  doi:10.1021/acs.orglett.5b01194
  • El-Khoury PZ, Y Gong, P Abellan, BW Arey, AG Joly, D Hu, JE Evans, ND Browning, and WP Hess. 2015. "Tip-Enhanced Raman Nanographs: Mapping Topography and Local Electric Fields." Nano Letters 15(4):2385-2390.  doi:10.1021/acs.nanolett.5b00609
  • Grate JW, KF Mo, Y Shin, A Vasdekis, MG Warner, RT Kelly, G Orr, D Hu, KJ Dehoff, FJ Brockman, and MJ Wilkins. 2015. "Alexa Fluor-labeled Fluorescent Cellulose Nanocrystals for Bioimaging Solid Cellulose in Spatially Structured Microenvironments." Bioconjugate Chemistry 26(3):593-601.  doi:10.1021/acs.bioconjchem.5b00048
  • Jacobson KH, IL Gunsolus, TR Kuech, JM Troiano, ES Melby, SE Lohse, D Hu, WB Chrisler, C Murphy, G Orr, FM Geiger, CL Haynes, and JA Pedersen. 2015. "Lipopolysaccharide density and structure govern the extent and distance of nanoparticle interaction with actual and model bacterial outer membranes." Environmental Science & Technology 49(17):10642-10652.  doi:10.1021/acs.est.5b01841
  • Liu TL, JT Cox, D Hu, X Deng, JZ Hu, MY Hu, J Xiao, Y Shao, K Tang, and J Liu. 2015. "A Fundamental Study on the [(µ-Cl)3Mg2(THF)6]+ Dimer Electrolytes for Rechargeable Mg Batteries." Chemical Communications 51(12):2312-2315.  doi:10.1039/c4cc07621d
  • Lu N, A Massoudieh, X Liang, D Hu, T Kamai, TR Ginn, JL Zilles, and TH Nguyen. 2015. "Swimming motility reduces Azotobacter vinelandii deposition to silica surfaces." Journal of Environmental Quality 44(5):1366-1375.  doi:10.2134/jeq2015.03.0141
  • Mihai C, WB Chrisler, Y Xie, D Hu, CJ Szymanski, A Tolic, J Klein, JN Smith, BJ Tarasevich, and G Orr. 2015. "Intracellular accumulation dynamics and fate of zinc ions in alveolar epithelial cells exposed to airborne ZnO nanoparticles at the air-liquid interface." Nanotoxicology 9(1):9-22.  doi:10.3109/17435390.2013.859319
  • Szymanski CJ, P Munusamy, C Mihai, Y Xie, D Hu, MK Gilles, T Tyliszczak, S Thevuthasan, DR Baer, and G Orr. 2015. "Shifts in oxidation states of cerium oxide nanoparticles detected inside intact hydrated cells and organelles." Biomaterials 62:147-154.  doi:10.1016/j.biomaterials.2015.05.042
  • Troiano J, LL Olenick, TR Kuech, ES Melby, D Hu, SE Lohse, AC Mensch, M Dogangun, AM Vartanian, M Torelli, E Ehimiaghe, SR Walter, L Fu, CR Anderton, Z Zhu, H Wang, G Orr, C Murphy, RJ Hamers, JA Pedersen, and FM Geiger. 2015. "Direct Probes of 4 nm Diameter Gold Nanoparticles Interacting with Supported Lipid Bylayers." Journal of Physical Chemistry C 119(1):534-546.  doi:10.1021/jp512107z
  • Wang Z, B Liu, E Zhao, K Jin, Y Du, JJ Neeway, JV Ryan, D Hu, H Zhang, M Hong, S Le Guernic, S Thevuthasan, F Wang, and Z Zhu. 2015. "Argon Cluster Sputtering Source for ToF-SIMS Depth Profiling of Insulating Materials: High Sputter Rate and Accurate Interfacial Information." Journal of the American Society for Mass Spectrometry 26(8):1283-1290.  doi:10.1007/s13361-015-1159-1


  • El-Khoury PZ, TW Ueltschi, AL Mifflin, D Hu, and WP Hess. 2014. "Frequency Resolved Nanoscale Chemical Imaging of 4,4'-Dimercaptostilbene on Silver." Journal of Physical Chemistry C 118(47):27525-27530.  doi:10.1021/jp509082c
  • Cole JK, JR Hutchison, RS Renslow, YM Kim, WB Chrisler, HE Engelmann, A Dohnalkova, D Hu, TO Metz, JK Fredrickson, and SR Lindemann. 2014. "Phototrophic Biofilm Assembly in Microbial-Mat-Derived Unicyanobacterial Consortia: Model Systems for the Study of Autotroph-Heterotroph Interactions." Frontiers in Microbiology 5:Article No. 109.  doi:10.3389/fmicb.2014.00109
  • El-Khoury PZ, E Khon, Y Gong, AG Joly, P Abellan, JE Evans, ND Browning, D Hu, M Zamkov, and WP Hess. 2014. "Electric Field Enhancement in a Self-Assembled 2D Array of Silver Nanospheres." Journal of Chemical Physics 141(21):Article No. 214308.  doi:10.1063/1.4902905
  • Gunsolus IL, D Hu, C Mihai, SE Lohse, CS Lee, M Torelli, RJ Hamers, C Murphy, G Orr, and CL Haynes. 2014. "Facile method to stain the bacterial cell surface for super-resolution fluorescence microscopy." Analyst 139(12):3174-3178.  doi:10.1039/c4an00574k


  • El-Khoury PZ, D Hu, and WP Hess. 2013. "Junction Plasmon-Induced Molecular Reorientation." Journal of Physical Chemistry Letters 4(20):3435-3439.  doi:10.1021/jz401838b
  • El-Khoury PZ, D Hu, VA Apkarian, and WP Hess. 2013. "Raman Scattering at Plasmonic Junctions Shorted by Conductive Molecular Bridges." Nano Letters 13(4):1858-1861.  doi:10.1021/nl400733r
  • El-Khoury PZ, SJ Peppernick, D Hu, AG Joly, and WP Hess. 2013. "The Origin of Surface-Enhanced Raman Scattering of 4,4' -Biphenyldicarboxylate on Silver Substrates." Journal of Physical Chemistry C 117(14):7260-7268.  doi:10.1021/jp401026x
  • Varga T, TC Droubay, ME Bowden, RJ Colby, S Manandhar, V Shutthanandan, D Hu, BC Kabius, E Apra, WA Shelton, and SA Chambers. 2013. "Coexistence of Weak Ferromagnetism and Polar Lattice Distortion in Epitaxial NiTiO3 thin films of the LiNbO3-Type Structure." Journal of Vacuum Science and Technology B--Microelectronics and Nanometer Structures 31(3):Article No. 030603.  doi:10.1116/1.4801664
  • Brown E, D Hu, N Abu-Lail, and X Zhang. 2013. "Potential of nanocrystalline cellulose-fibrin nanocomposites for artificial vascular graft applications." Biomacromolecules 14(4):1063-1071. 
  • Chien MP, AS Carlini, D Hu, CV Barback, AM Rush, DJ Hall, G Orr, and NC Gianneschi. 2013. "Enzyme-Directed Assembly of Nanoparticles in Tumors Monitored by In Vivo Whole Animal and Ex Vivo Super-Resolution Fluorescence Imaging." Journal of the American Chemical Society 135(50):18710-18713.  doi:10.1021/ja408182p
  • Geng H, S Pan, and D Hu. 2013. "Electrogenerated Chemiluminescence and Fluorescence Lifetime Spatial Heterogeneity of Poly (2-methoxy-5-(2-ethylhexyloxy)-1, 4-phenylenevinylene) in Presence of [6,6]-phenyl-C61-butyric Acid Methyl Ester." In MRS Proceedings: Symposium E/H - Photovoltaic Technologies, Devices and Systems Based on Inorganic Materials, Small Organic Molecules and Hybrids , vol. 1493, pp. Paper No. mrsf12-1493-h01-09.  Materials Research Society, Warrendale, PA.  doi:10.1557/opl.2013.19
  • Hu D, B Zhao, Y Xie, G Orr, and AD Li. 2013. "Understanding Super-Resolution Nanoscopy and Its Biological Applications in Cell Imaging." Physical Chemistry Chemical Physics. PCCP 15(36):14856-14861.  doi: 10.1039/C3CP51629F
  • Liu Y, C Zhang, D Hu, MS Kuhlenschmidt, TB Kuhlenschmidt, SE Mylon, R Kong, R Bhargava, and TH Nguyen. 2013. "Role of collector alternating charged patches on transport of Cryptosporidium parvum oocyst in a patchwise charged heterogeneous micromodel." Environmental Science & Technology 47(6):2670-2678.  doi:10.1021/es304075j
  • Nasybulin EN, W Xu, MH Engelhard, XS Li, M Gu, D Hu, and J Zhang. 2013. "Electrocatalytic properties of poly(3,4-ethylenedioxythiophene) (PEDOT) in Li-O2 battery." Electrochemistry Communications 29:63-66.  doi:10.1016/j.elecom.2013.01.011
  • Wang Y, Z Li, TJ Weber, D Hu, CT Lin, J Li, and Y Lin. 2013. "In Situ Live Cell Sensing of Multiple Nucleotides Exploiting DNA/RNA Aptamers and Graphene Oxide Nanosheets." Analytical Chemistry 85(14):6775-6782.  doi:10.1021/ac400858g


  • Jin H, Y Yu, WB Chrisler, Y Xiong, D Hu, and C Lei. 2012. "Delivery of MicroR A-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing." Breast Cancer: Basic and Clinical Research 6:9-19.  doi:10.4137/BCBCR.S8513
  • Chen X, W Xu, J Xiao, MH Engelhard, F Ding, D Mei, D Hu, J Zhang, and J Zhang. 2012. "Effects of cell positive cans and separators on the performance of high-voltage Li-ion batteries." Journal of Power Sources 213:160-168.  doi:10.1016/j.jpowsour.2012.04.009
  • Clayton DA, T McPherson, S Pan, M Chen, DA Dixon, and D Hu. 2012. "Spatial and temporal variation of surface-enhanced Raman scattering at Ag nanowires in aqueous solution." Physical Chemistry Chemical Physics. PCCP 15(3):850-859. 
  • Xu W, AL Read, PK Koech, D Hu, CM Wang, J Xiao, AB Padmaperuma, GL Graff, J Liu, and J Zhang. 2012. "Factors Affecting the Battery Performance of Anthraquinone-based Organic Cathode Materials." Journal of Materials Chemistry 22(9):4032-4039.  doi:10.1039/c2jm15764k


  • Li AD, C Zhan, D Hu, W Wan, and J Yao. 2011. "Photoswitchable Nanoprobes Offer Unlimited Brightness in Frequency-Domain Imaging." Journal of the American Chemical Society 133(20):7628-7631.  doi:10.1021/ja1108479
  • Tian Z, AD Li, and D Hu. 2011. "Super-Resolution Fluorescence Nanoscopy Applied to Image Core-Shell Photoswitching Nanoparticles and their Self-Assemblies ." Chemical Communications 47(4):1258-1260.  doi:10.1039/C0CC03217D
  • Xiao J, JZ Hu, D Wang, D Hu, W Xu, GL Graff, Z Nie, J Liu, and J Zhang. 2011. "Investigation of the Rechargeability of Li-O2 Batteries in Non-aqueous Electrolyte." Journal of Power Sources 196(13):5674-5678. 
  • Xu W, K Xu, VV Viswanathan, SA Towne, JS Hardy, J Xiao, Z Nie, D Hu, D Wang, and J Zhang. 2011. "Reaction Mechanisms for the Limited Reversibility of Li-O2 Chemistry in Organic Carbonate Electrolytes." Journal of Power Sources 196(22):9631-9639.  doi:10.1016/j.jpowsour.2011.06.099
  • Xu W, VV Viswanathan, D Wang, SA Towne, J Xiao, Z Nie, D Hu, and J Zhang. 2011. "Investigation on the Charging Process of Li2O2-Based Air Electrodes in Li-O2 Batteries with Organic Carbonate Electrolytes." Journal of Power Sources 196(8):3894-3899. 


  • Hu D, and G Orr. 2010. "Nanometer Resolution Imaging by SIngle Molecule Switching." Nano Reviews 1:5122.  doi:10.3402/nano.v1i0.5122
  • Li G, D Hu, G Xia, and ZC Zhang. 2010. "Catalyst Structure-Performance Relationship Identified by High-Throughput Operando Method: New Insight for Silica-Supported Vanadium Oxide for Methanol Oxidation." Topics in Catalysis 53(1-2):40-48. 
  • Wang D, R Kou, D Choi, Z Yang, Z Nie, J Li, LV Saraf, D Hu, J Zhang, GL Graff, J Liu, MA Pope, and IA Aksay. 2010. "Ternary Self-Assembly of Ordered Metal Oxide-Graphene Nanocomposites for Electrochemical Energy Storage ." ACS Nano 4(3):1587-1595.  doi:10.1021/nn901819n
  • Wang Y, Z Li, D Hu, CT Lin, J Li, and Y Lin. 2010. "Aptamer/Graphene Oxide Nanocomplex for In Situ Molecular Probing in Living Cells." Journal of the American Chemical Society 132(27):9274-9276.  doi:10.1021/ja103169v


  • Hu D, Z Tian, W Wu, W Wan, and AD Li. 2009. "Single-Molecule Photoswitching Enables High-Resolution Optical Imaging." In Microscopy and Microanalysis , vol. 15, no. Suppl. 2, pp. 840-841.  Cambridge University Press, Cambridge, United Kingdom. 
  • Lei C, D Hu, and EJ Ackerman. 2009. "Clay Nanoparticle-Supported Single-Molecule Fluorescence Spectroelectrochemistry." Nano Letters 9(2):655-658. 
  • Hu D, C Lei, and EJ Ackerman. 2009. "Single-molecule electron transfer reactions in nanomaterials." In Microscopy and Microanalysis 2009: Proceedings, July 26-30, 2009, Richmond, Virginia, vol. 15, no. Suppl 2, ed. LN Brewer, pp. 1138-1139.  Cambridge University Press, New York.  doi:10.1017/S1431927609095178
  • Li G, D Hu, G Xia, and ZC Zhang. 2009. "Methanol Partial Oxidation on MoO3/SiO2 Catalysts: Application of Vibrational Spectroscopic Imaging Techniques in a High Throughput Operando Reactor." Topics in Catalysis 52:1381-1387. 
  • Wang D, D Choi, J Li, Z Yang, Z Nie, R Kou, D Hu, CM Wang, LV Saraf, J Zhang, IA Aksay, and J Liu. 2009. "Self-assembled TiO2-Graphene Hybrid Nanostructures for Enhanced Li-ion Insertion ." ACS Nano 3(4):907-914. 


  • Hu D, Z Tian, W Wu, W Wan, and AD Li. 2008. "Photoswitchable Nanoparticles Enable High-Resolution Cell Imaging: PULSAR Microscopy." Journal of the American Chemical Society 130(46):15279-15281.  doi:10.1021/ja805948u
  • Lei C, D Hu, and EJ Ackerman. 2008. "Single-molecule Fluorescence Spectroelectrochemistry of Cresyl Violet." Chemical Communications (43):5490-5492.  doi:10.1039/b812161c
  • Li G, D Hu, G Xia, JM White, and ZC Zhang. 2008. "High Throughput Operando Studies using Fourier Transform Infrared Imaging and Raman Spectroscopy." Review of Scientific Instruments 79:074101.  doi:10.1063/1.2949389


  • Orr G, DJ Panther, JL Phillips, BJ Tarasevich, A Dohnalkova, D Hu, JG Teeguarden, and JG Pounds. 2007. "Submicron and Nanoscale Inorganic Particles Exploit the Actin Machinery to be Propelled Along Microvillilike Structures into Alveolar Cells." ACS Nano 1(5):463-475.  doi:10.1021/nn700149r
  • Pan D, D Hu, R Liu, X Zeng, S Kaplan, and HP Lu. 2007. "Fluctuating Two-State Light Harvesting in a Photosynthetic Membrane." Journal of Physical Chemistry C 111(25):8948-8956.  doi:10.1021/jp071493y


  • Hu D, and HP Lu. 2006. "Single Molecule Electron Transfer Process of Ruthenium Complexes." In Ultrasensitive and Single-Molecule Detection Technologies, 21-22 and 24 January 2006, San Jose, California, USA . Proceedings of the SPIE, vol. 6092, ed. Jörg Enderlein and Zygmunt K. Gryczynski, pp. 60927-1 -- 60927-7.  Society of Photo-optical Instrumentation Engineers, Bellingham, WA. 
  • Liu R, D Hu, X Tan, and HP Lu. 2006. "Revealing Two-State Protein-Protein Interaction of Calmodulin by Single-Molecule Spectroscopy." Journal of the American Chemical Society 128(31):10034-10042. 
  • Pan D, NA Klymyshyn, D Hu, and HP Lu. 2006. "Tip-Enhanced Near-Field Raman Spectroscopy Probing Single Dye-Sensitized TiO2 Nanoparticles." Applied Physics Letters 88(9):Art. No. 093121. 
  • Zhang T, SN Danthi, J Xie, D Hu, HP Lu, and KH Li. 2006. "Live Cell Imaging of the Endocytosis and the Intracellular Trafficking of Multifunctional Lipid Nanoparticles." In Proceedings of the SPIE, vol. 6095, p. Art. No. 6095D.  S P I E, Bellingham, WA. 


  • Hu D, and HP Lu. 2005. "Single-Molecule Triplet-State Photon Antibunching at Room Temperature." Journal of Physical Chemistry B 109(20):9861-9864. 
  • Orr G, D Hu, S Ozcelik, L Opresko, HS Wiley, and SD Colson. 2005. "Cholesterol Dictates the Freedom of EGF Receptors and HER2 in the Plane of the Membrane." Biophysical Journal 89(2):1362-1373.  doi:10.1529/biophysj.104.056192
  • Link S, D Hu, WS Chang, GD Scholes, and PF Barbara. 2005. "Nernatic Solvation of Segmented Polymer Chains." Nano Letters 5(9):1757-1760.  doi:10.1021/nl051108l
  • Pan D, D Hu, and HP Lu. 2005. "Probing Inhomogeneous Vibrational Reorganization Energy Barriers of Interfacial Electron Transfer." Journal of Physical Chemistry B 109(34):16390-16395. 


  • Hu D, and HP Lu. 2004. "Placing Single-Molecule T4 Lysozyme Enzymes on a Bacterial Cell Surface: Toward Probing Single-Molecule Enzymatic Reaction in Living Cells." Biophysical Journal 87:656-661. 
  • Hu D, M Micic, NA Klymyshyn, YD Suh, and HP Lu. 2004. "Correlated Topographic and Spectroscopic Imaging by Combined Atomic Force Microscopy and Optical Microscopy." Journal of Luminescence 107(1-4):4-12. 
  • Biju VP, M Micic, D Hu, and HP Lu. 2004. "Intermittent Single-Molecule Interfacial Electron Transfer Dynamics." Journal of the American Chemical Society 126(30):9374-9381. 
  • Micic M, D Hu, YD Suh, GJ Newton, MF Romine, and HP Lu. 2004. "Correlated Atomic Force Microscopy and Flourescence Lifetime Imaging of Live Bacterial Cells." Colloids and Surfaces. B, Biointerfaces 34:205-212. 
  • Tan X, D Hu, TC Squier, and HP Lu. 2004. "Probing Nanosecond Protein Motions of Calmodulin by Single-Molecule Fluorescence Anisotropy." Applied Physics Letters 85(12):2420-2422. 
  • Tan X, D Hu, TC Squier, and HP Lu. 2004. "Probing Nanosecond Protein Motions of Calmodulin by Single-Molecule Fluorescence Anisotropy." Applied Physics Letters 85(12):2420-2422. 
  • Tan X, P Nalbant, A Toutchkine, D Hu, ER Vorpagel, KM Hahn, and HP Lu. 2004. "Single-Molecule Study of Protein-Protein Interaction Dynamics in a Cell Signaling System." Journal of Physical Chemistry B 108(2):737-744. 


  • Hu D, and HP Lu. 2003. "Single-Molecule Nanosecond Anisotropy Dynamics of Tethered Protein Motions." Journal of Physical Chemistry B 107(2):618-626. 
  • Hu D, M Micic, NA Klymyshyn, YD Suh, and HP Lu. 2003. "Correlated Topographic and Spectroscopic Imaging Beyond Diffraction Limit by Atomic Force Microscopy Metallic Tip-Enhanced Near-Field Flourescence Lifetime Microscopy." Review of Scientific Instruments 74(7):3347-3355. 
  • Chen Y, D Hu, ER Vorpagel, and HP Lu. 2003. "Probing Single-Molecule T4 Lysozyme Conformational Dynamics by Intramolecular Fluorescence Energy Transfer." Journal of Physical Chemistry B 107:7947-7956. 

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