Staff information

PNNL Publications

2019

• Hess B.M., D.G. Thomas, T.J. Weber, J.R. Hutchison, T.M. Straub, C.J. Bruckner-Lea, and J.D. Powell, et al. 2019. "An integrated experimental-computational approach for predicting virulence in New Zealand white rabbits and humans following inhalation exposure to Bacillus anthracis spores." PLoS Pathogens 14, no. 7:e0219160. PNNL-SA-140673. doi:10.1371/journal.pone.0219160

2018

• Leiser O.P., J. Blackburn, T.L. Hadfield, H.W. Kreuzer, D.S. Wunschel, and C.J. Bruckner-Lea. 2018. "Laboratory strains of Bacillus anthracis exhibit pervasive alteration in expression of proteins related to sporulation under laboratory conditions relative to genetically related wild strains." PLoS One 13, no. 12:Article Number e0209120. PNNL-SA-137549. doi:10.1371/journal.pone.0209120

2017

• Bartholomew R.A., R.M. Ozanich, J.S. Arce, H.E. Engelmann, A. Heredia-Langner, B.A. Hofstad, and J.R. Hutchison, et al. 2017. "Evaluation of Immunoassays and General Biological Indicator Tests for Field Screening of Bacillus anthracis and Ricin." Health Security 15, no. 1:81-96. PNNL-SA-116267. doi:10.1089/hs.2016.0044
• Ozanich R.M., H.A. Colburn, K.D. Victry, R.A. Bartholomew, J.S. Arce, A. Heredia-Langner, and K.H. Jarman, et al. 2017. "Evaluation of PCR Systems for Field Screening of Bacillus anthracis." Health Security 15, no. 1:70-80. PNNL-SA-115228. doi:10.1089/hs.2016.0043
• Ozanich R.M., R.A. Bartholomew, and C.J. Bruckner-Lea. 2017. "Response to Letter on Immunoassays for Field Screening of Bacillus anthracis and Ricin." Health Security 15, no. 2:222. PNNL-SA-124365. doi:10.1089/hs.2017.0011.resp

2016

• Straub T.M., D.R. Call, C.J. Bruckner-Lea, H.A. Colburn, C.L. Baird, R.A. Bartholomew, and R.M. Ozanich, et al. 2016. "Field application of pathogen detection technologies." In Manual of Environmental Microbiology 4th edition, edited by Yates, MV. PNNL-SA-95424. doi:10.1128/9781555818821.ch2.3.4

2015

• Hess B.M., B. Kaiser, M.A. Sydor, D.S. Wunschel, C.J. Bruckner-Lea, and J.R. Hutchison. 2015. "Discerning Viable from Nonviable Yersinia pestis pgm- and Bacillus anthracis Sterne using Propidium Monoazide in the Presence of White Powders." Journal of Bioterrorism and Biodefense 6, no. 3:Article No. 138. PNNL-SA-107253.
• Ozanich R.M., C.J. Bruckner-Lea, and R.A. Bartholomew. 2015. "National Laboratory Support for First Responders’ Biodetection Needs." DomPrep Journal 11, no. 7:33-37. PNNL-SA-114714.

2013

• Seiner D.R., H.A. Colburn, C.L. Baird, R.A. Bartholomew, T.M. Straub, K.D. Victry, and J.R. Hutchison, et al. 2013. "Evaluation of the FilmArray® system for detection of Bacillus anthracis, Francisella tularensis, and Yersinia pestis." Journal of Applied Microbiology 114, no. 4:992-1000. PNNL-SA-87496. doi:10.1111/jam.12107
• Straub T.M., C.L. Baird, R.A. Bartholomew, H.A. Colburn, D.R. Seiner, K.D. Victry, and L. Zhang, et al. 2013. "Estimated Copy Number of Bacillus anthracis Plasmids pXO1 and pXO2 using Digital PCR." Journal of Microbiological Methods 92, no. 1:9-10. PNNL-SA-84286. doi:10.1016/j.mimet.2012.10.013
• Straub T.M., J.R. Hutchison, R.A. Bartholomew, C.O. Valdez, N.B. Valentine, A. Dohnalkova, and R.M. Ozanich, et al. 2013. "Defining cell culture conditions to improve human norovirus infectivity assays." Water Science and Technology 67, no. 4:863-868. PNNL-SA-88268. doi:10.2166/wst.2012.636

2012

• Corley C.D., M.J. Lancaster, R.T. Brigantic, J.S. Chung, R.A. Walters, R. Arthur, and C.J. Bruckner-Lea, et al. 2012. "Assessing the Continuum of Event-Based Biosurveillance Through an Operational Lens." Biosecurity and Bioterrorism: Biodefense Strategy, Practice and Science 10, no. 1:131-141. PNNL-SA-77400. doi:10.1089/bsp.2011.0096

2011

• Straub T.M., R.A. Bartholomew, C.O. Valdez, N.B. Valentine, A. Dohnalkova, R.M. Ozanich, and C.J. Bruckner-Lea, et al. 2011. "Human Norovirus Infection of Caco-2 Cells Grown as a Three-Dimensional Tissue Structure." Journal of Water and Health 9, no. 2:225-240. PNWD-SA-8851.

2010

• Grate J.W., R.M. Ozanich, M.G. Warner, C.J. Bruckner-Lea, and J.D. Marks. 2010. "Advances in Assays and Analytical Approaches for Botulinum Toxin Detection." Trends in Analytical Chemistry. TrAC 29, no. 10:1137-1156. PNNL-SA-72191. doi:10.1016/j.trac.2010.07.005

2009

• Grate J.W., M.G. Warner, R.M. Ozanich, K.D. Miller, H.A. Colburn, B.P. Dockendorff, and K.C. Antolick, et al. 2009. "Renewable Surface Fluorescence Sandwich Immunoassay Biosensor for Rapid Sensitive Botulinum Toxin Detection in an Automated Fluidic Format." Analyst 134, no. 5:987 - 996. PNNL-SA-63257. doi:10.1039/B900794F
• Ozanich R.M., C.J. Bruckner-Lea, M.G. Warner, K.D. Miller, K.C. Antolick, J.D. Marks, and J. Lou, et al. 2009. "Rapid Multiplexed Flow Cytometric Assay for Botulinum Neurotoxin Detection Using an Automated Fluidic Microbead-Trapping Flow Cell for Enhanced Sensitivity." Analytical Chemistry 81, no. 14:5783-5793. PNNL-SA-64163.
• Ozanich R.M., K.C. Antolick, C.J. Bruckner-Lea, K.J. Bunch, B.P. Dockendorff, J.W. Grate, and M.A. Nash, et al. 2009. "Bead-Based Assays for Biodetection: From Flow-Cytometry to Microfluidics." In Proceedings of the SPIE: Optics and Photonics in Global Homeland Security V and Biometric Technology for Human Identification VI, edited by CS Halvorson, et al, 7306, Art. No. 730601. Bellingham, Washington:SPIE. PNNL-SA-65500. doi:10.1117/12.819951
• Warner C.L., C.J. Bruckner-Lea, J.W. Grate, T.M. Straub, G.J. Posakony, N.B. Valentine, and R.M. Ozanich, et al. 2009. "A Flow-Through Ultrasonic Lysis System for the Disruption of Bacterial Spores." JALA. Journal of the Association for Laboratory Automation 14, no. 5:277-284. PNNL-SA-58098.
• Warner M.G., J.W. Grate, A.J. Tyler, R.M. Ozanich, K.D. Miller, J. Lou, and J.D. Marks, et al. 2009. "Quantum dot immunoassays in renewable surface column and 96-well plate formats for the fluorescence detection of Botulinum neurotoxin using high-affinity antibodies." Biosensors and Bioelectronics 25, no. 1:179-184. PNNL-SA-64430.

2007

• Ozanich R.M., K.C. Antolick, C.J. Bruckner-Lea, B.P. Dockendorff, A.N. Easterday, H.C. Edberg, and J.W. Grate, et al. 2007. "Use of a Novel Fluidics Microbead Trap/Flow-cell Enhances Speed and Sensitivity of Bead-Based Bioassays." JALA. Journal of the Association for Laboratory Automation 12, no. 5:303-310. PNNL-SA-54715. doi:10.1016/j.jala.2007.05.002
• Straub T.M., K.A. Honer Zu Bentrup, P. Orosz Coghlan, A. Dohnalkova, B.K. Mayer, R.A. Bartholomew, and C.O. Valdez, et al. 2007. "Cell Culture Assay for Human Noroviruses [response]." Emerging Infectious Diseases 13, no. 7:1117-1118. PNWD-SA-7838. Cell Culture Assay for Human Noroviruses [response]
• Straub T.M., K.A. Honer Zu Bentrup, P.A. Orosz Coghlan, A. Dohnalkova, B.K. Mayer, R.A. Bartholomew, and C.O. Valdez, et al. 2007. "In Vitro Cell Culture Infectivity Assay for Human Noroviruses." Emerging Infectious Diseases 13, no. 3:396-403. PNNL-SA-53115.

2006

• Varnum S.M., M.G. Warner, B.P. Dockendorff, N.C. Anheier, J. Lou, J.D. Marks, and L.A. Smith, et al. 2006. "Enzyme-amplified protein micorarray and a fluidic renewable surface fluorescence immunoassay for botulinum neurotoxin detection using high-affinity recombinant antibodies." Analytica Chimica Acta 570, no. 2:137-143. PNNL-SA-47972.

2005

• Bruzek A.K., and C.J. Bruckner-Lea. 2005. "Automated RNA Extraction and Purification for Multiplexed Pathogen Detection." Journal of Undergraduate Research V, no. 2005:112. PNNL-SA-42407.
• Straub T.M., B.P. Dockendorff, M.D. Quinonez-Diaz, C.O. Valdez, J.I. Shutthanandan, B.J. Tarasevich, and J.W. Grate, et al. 2005. "Automated Methods for Multiplexed Pathogen Detection." Journal of Microbiological Methods 62, no. 3:303-316. PNNL-SA-41426.

2004

• Bruckner-Lea C.J. 2004. "Biosensor Systems for Homeland Security." Electrochemical Society Interface 13, no. 2:36. PNNL-SA-41537.
• Skaggs R., T.M. Straub, B. Wright, C.J. Bruckner-Lea, and S.D. Harvey. 2004. "Development of the Next Generation Microbiological and Chemical Detection Capabilities for Water Supplies." In Water Supply Systems Security, edited by Larry W. Mays. 18.1-18.21. New York, New York:McGraw Hill. PNNL-SA-40766.
• Warner M.G., B.P. Dockendorff, M.J. Feldhaus, N.C. Anheier, J.D. Marks, J.W. Grate, and C.J. Bruckner-Lea. 2004. "Rapid, Sensitive Detection of Botulinum Toxin on a Flexible Microfluidics Platform." In Chemical Sensors VI: Chemical and Biological Sensors and Analytical Methods, edited by C. Bruckner-Lea, P. Vanysek, G. Hunter, et al., 2004-08, 376-380. Pennington, New Jersey:Electrochemical Society. PNNL-SA-41899.

2003

• Grate J.W., C.J. Bruckner-Lea, A.E. Jarrell, and D.P. Chandler. 2003. "Automated Sample Preparation method for suspension arrays using renewable surface separations with multiplexed flow cytometry fluorescence detection." Analytica Chimica Acta 478, no. 1:85-98. PNNL-SA-36630.
• Grate J.W., C.J. Bruckner-Lea, A.E. Jarrell, and D.P. Chandler. 2003. "Erratum to: Automated Sample Preparation Method for Suspension Arrays using Renewable Surface Separations with Multiplexed Flow Cytometry Fluorescence Detection." Analytica Chimica Acta 482, no. 1:147. PNNL-SA-37670.

2002

• Bruckner-Lea C.J., D.P. Chandler, and J.W. Grate. 2002. "Renewable Surface Biosensors." In Proceedings of the International Symposium on Ultramicrochemical Process Systems - High Throughput Screening and Process-on-chip, February 25 - 26, 2002, Seoul, Korea, 10 pages. Seoul:Korea Advanced Institute of Science and Technology. PNWD-SA-5636.
• Bruckner-Lea C.J., T. Tsukuda, B.P. Dockendorff, J.C. Follansbee, M.T. Kingsley, C.O. Ocampo, and J.R. Stults, et al. 2002. "Renewable Microcolumns for Automated DNA Purification and Flow-through Amplification: From Sediment Samples through Polymerase Chain Reaction." Analytica Chimica Acta 469, no. 1:129-140. PNNL-SA-34985.

2001

• Bruckner-Lea C.J., D.P. Chandler, G.J. Posakony, and L.J. Bond. 2001. "Continuous Spore Disruption for DNA analysis using Radially-Focused, High-Frequency Ultrasound." Analytical Chemistry 73, no. 15:3784-3789. PNWD-SA-5148-A.
• Bruckner-Lea C.J., E.J. Ackerman, B.P. Dockendorff, D.A. Holman, and J.W. Grate. 2001. "Renewable Surface Biosensors with Optical Detection." In Electrochemical Society Symposium Proceedings: Chemical and Biological Sensors and Analytical Methods II, edited by MA Butler, P Vanysek and N Yamazoe, 157-164. Pennington, New Jersey:The Electrochemical Society. PNNL-SA-34650.
• Bruckner-Lea C.J., E.J. Ackerman, B.P. Dockendorff, D.A. Holman, and J.W. Grate. 2001. "Renewable Surface Biosensors With Optical Detection." In Joint International Meeting - the 200th Meeting of The Electrochemical Society, Inc. and the 52nd Annual Meeting of the International Society of Electrochemistry, September 2-7, 2001, San Francisco, California. Pennington, New Jersey:The Electrochemical Society. PNNL-SA-34465.
• Chandler D.P., J.D. Brown, C.J. Bruckner-Lea, L. Olson, G.J. Posakony, J.R. Stults, and N.B. Valentine, et al. 2001. "Continuous Spore Disruption Using Radially Focused, High-Frequency Ultrasound." Analytical Chemistry 73, no. 15:3784-3789. PNNL-SA-33210.
• Chandler D.P., J.D. Brown, D.R. Call, S.C. Wunschel, J.W. Grate, D.A. Holman, and L.G. Olson, et al. 2001. "Automated Immunomagnetic Separation and Microarray Detection of E. coli O157:H7 from Poultry Carcass Rinse." International Journal of Food Microbiology 70, no. 1-2:143-154. PNWD-SA-5123.

2000

• Bruckner-Lea C.J., G.K. Celler, R.G. Kelly, J. Lessard, R.D. Mcconnell, K. Niki, and K. Rajeshwar. 2000. "Technology in the Next Century: Reflections on Electrochemistry and Solid-State Science and Technology." Electrochemical Society Interface 9, no. 1:20-29. PNNL-SA-32965.
• Bruckner-Lea C.J., L. Brown, D.A. Holman, L. Olson, and J.W. Grate. 2000. "Biomolecular Interaction Assay." In Meeting Abstracts. The 198th Meeting of the Electrochemical Society, Hyatt Regency Phoenix, October 22-27, 2000, 2002-2, 1180. Phoenix, Arizona:The Electrochemical Society. PNNL-SA-33267-A.
• Bruckner-Lea C.J., M.R. Stottlemyre, D.A. Holman, J.W. Grate, F.J. Brockman, and D.P. Chandler. 2000. "Rotating Rod Renewable Microcolums for Sutomated, Solid-Phase DNA Hybridization." Analytical Chemistry 72, no. 17:4135-4141. PNNL-SA-32763.
• Bruckner-Lea C.J., N.C. Anheier, D.A. Holman, T. Tsukuda, M.T. Kingsley, F.J. Brockman, and J.M. Price, et al. 2000. "Integrated Systems for DNA Sample Preparation and Detection in Environmental Samples." In Biochemical and Biomolecular Sensing, SPIE Proceedings, 6-7 November 2000, Boston, USA, edited by Robert A. Lieberman, 4200, 74-81. Bellingham, Washington:International Society for Optical Engineering. PNNL-SA-32983.

1999

• Bruckner-Lea C.J., D.A. Holman, B.L. Schuck, F.J. Brockman, and D.P. Chandler. 1999. "Strategies for Automated Sample Preparation, Nucleic Acid Purification, and Concentration of Low-Target-Number Nucleic Acids in Environmental and Food Processing Samples." In Pathogen Detection and Remediation for Safe Eating; SPIE Proceedings, edited by Yud-Ren Chen, 3544, 63-71. Bellingham, Washington:SPIE- The International Society for Optical Engineering. PNNL-SA-30432.
• Chandler D.P., B.L. Schuck, F.J. Brockman, and C.J. Bruckner-Lea. 1999. "Automated Nucleic Acid Isolation and Purification from Soil Extracts Using Renewable Affinity Microcolumns in a Sequential Injection System." Talanta 49, no. 5:969-983. PNNL-SA-30363.
• Chandler D.P., D.A. Holman, F.J. Brockman, J.W. Grate, and C.J. Bruckner-Lea. 1999. "Renewable microcolumns for solid-phase nucleic acid separations and analysis from environmental samples." Trends in Analytical Chemistry. TrAC 19, no. 5:314-321. PNNL-SA-32197.
• Grate J.W., O.B. Egorov, and C.J. Bruckner-Lea. 1999. "Sequential Injection Separation and Sensing." In Chemical Microsensors and Applications II, Proceedings of SPIE, edited by S. Buttgenbach, 3857, 70-75. Bellingham, Washington:SPIE-The International Society for Optical Engineering. PNNL-SA-30966.

1996

• Conroy J.F., K. Caldwell, C.J. Bruckner-Lea, and J. Janata. 1996. "Effects of Interfacial Tension in Tunneling Microscopy. 1. Theory." Journal of Physical Chemistry 100, no. 46:18222-18228. PNNL-SA-27448. doi:10.1021/jp962085p

1995

• Bruckner-Lea C.J., R.J. Kimmel, J. Janata, J.F. Conroy, and K. Caldwell. 1995. "Electrochemical Studies of Octadecanethiol and Octanethiol Films on Variable Surface Area Mercury Sessile Drops." Electrochimica Acta 40, no. 18:2897-2904. PNNL-SA-26262.