Quantitative proteomics analysis of cortical samples of 10 Alzheimer’s disease (AD) brains versus 10 normally aged brains was performed by following the accurate mass and time tag (AMT) approach with the high resolution LTQ Orbitrap mass spectrometer. More than 1400 proteins were identified and quantitated. A conservative approach of selecting only the consensus results of four normalization methods was suggested and used. A total of 197 proteins were shown to be significantly differentially abundant (p-values
Revised: December 19, 2012 |
Published: June 17, 2012
Citation
Andreev V.P., V.A. Petyuk, H.M. Brewer, Y. Karpievitch, F. Xie, J. Clarke, and D.G. Camp, et al. 2012.Label-Free Quantitative LC-MS Proteomics of Alzheimer’s Disease and Normally Aged Human Brains.Journal of Proteome Research 11, no. 6:3053-3067.PNNL-SA-90452.doi:10.1021/pr3001546