PNNL

Abstract

It is shown that high-resolution 1H NMR spectra of intact excised tissues and organs can be obtained by rotating the sample slowly about an axis at the magic angle of 54o44' with the external magnetic field. In this way tissue and cellular damage invoked by standard Magic Angle Spinning (MAS) experiments, where typically spinning speeds of several kHz are employed, are minimized. Special RF pulse sequences, that were developed originally in solid state NMR, can be used to produce a spinning sideband-free isotropic spectrum. In this paper the first results are shown of the brain, the heart, the liver, the gluteus muscle, and the kidney excised from mice, using the 2D-phase-altered spinning sidebands (PASS) technique and employing MAS spinning speeds of 43-125 Hz. It is found that with slow sample spinning similar, and in some cases even better, spectral resolutions are obtained as compared with fast MAS.