Leukemic stem cells are highly dynamic and heterogeneous. Analysis of leukemic stem cells at the single cell level should provide a wealth of insights that would not be possible using bulk measurements. Mass spectrometry (MS)-based proteomic workflows can quantify hundreds or thousands of proteins from a biological sample and has proven invaluable for biomedical research, but samples comprising large numbers of cells are typically required due to limited sensitivity. Recent developments in sample processing, chromatographic separations and MS instrumentation are now extending in-depth proteome profiling to single mammalian cells. We describe specific techniques that increase the sensitivity of single-cell proteomics by orders of magnitude, enabling the promise of single cell proteomics to become a reality. We anticipate such techniques could significantly advance our understanding of leukemic stem cells.
Revised: November 23, 2020 |
Published: November 10, 2020
Citation
Liang Y., T. Truong, Y. Zhu, and R.T. Kelly. 2020.In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomics. In Leukemia Stem Cells. Methods in Molecular Biology, edited by C. Cobaleda and I. Sanchez-Garcia. 159-179. New York, New York:Humana Press.PNNL-SA-148793.doi:10.1007/978-1-0716-0810-4_10