October 1, 2020
Journal Article

Cytotoxicity and cellular proteome impact of cellulose nanocrystals using simulated digestion and an in vitro small intestinal epithelium cellular model

Abstract

Cellulose nanocrystals (CNC), (0.75 or 1.5 % w/w) in either a fasting food model (FFM) or a standardized food model (SFM) based on the average USA diet, were digested using a three-phase (oral-gastric-small intestinal) digestion simulator, and the resulting small intestinal phase digests were applied to an in vitro triculture human cellular model of the small intestinal epithelium (SIE). Effects of exposures on SIE monolayer integrity, cytotoxicity, and oxidative stress were evaluated, and a global cellular proteomic analysis of exposed cells was performed to obtain a more comprehensive understanding of the cellular response to ingested CNC in different food models. Other than a slight increase in cytotoxicity (13.2%, p

Revised: November 16, 2020 | Published: October 1, 2020

Citation

Cao X., T. Zhang, G.M. Deloid, M.J. Gaffrey, K.K. Weitz, B.D. Thrall, and W. Qian, et al. 2020. Cytotoxicity and cellular proteome impact of cellulose nanocrystals using simulated digestion and an in vitro small intestinal epithelium cellular model. NanoImpact 20. PNNL-SA-157451. doi:10.1016/j.impact.2020.100269