Purpose: The ability to distinguish lung inflammation and fibrosis using signal intensity (S0) and T2 relaxation measured with magnetic resonance imaging (MRI) was investigated. Materials and Methods: Three groups of rats (n=5) were instilled intratracheally with bleomycin (2.5 U/kg or 3.5 U/kg) in saline or with saline only. Rats were imaged before dosing and 1, 2, 4, and 7 weeks after. Imaging was performed at 2.0 T using a multi-echo pulse sequence (TE=6 ms). S0 and T2 of dosed animals were calculated on a pixel-by-pixel basis and compared to controls. At week 7, rats were sacrificed, left lungs were prepared for histological analysis, and right lungs were used to measure water and hydroxyproline (collagen) content. Results: Results show that S0xT2 is significantly correlated with water and collagen content in HD rats (p
Revised: October 7, 2010 |
Published: May 1, 2010
Citation
Jacob R.E., B.G. Amidan, J.J. Soelberg, and K.R. Minard. 2010.In Vivo MRI of Altered Proton Signal Intensity and T2 Relaxation in a Bleomycin Model of Pulmonary Inflammation and Fibrosis.Journal of Magnetic Resonance Imaging 31, no. 5:1091-1099. PNWD-SA-8775. doi:10.1002/jmri.22166