This article describes developments in fundamental and applied aspects of separations, electrospray ionization phenomena, and mass spectrometric instrumentation that are interrelated and important for making more effective and quantitative measurements, particularly for proteomics applications. The basis for better quantitation and ultra-high sensitivity is highlighted for high resolution capillary liquid chromatography separations that provide low nL/min flow rates to an electrospray ionization interface. The increased dynamic range of measurements and sub-attomole regime detection limits obtainable open new avenues for biological research.
Revised: June 22, 2004 |
Published: April 1, 2004
Citation
Smith R.D., Y. Shen, and K. Tang. 2004.Ultra-sensitive and quantitative analyses from combined separations-mass spectrometry for the characterization of proteomes.Accounts of Chemical Research 37, no. 4:269-278.PNNL-SA-39791.